LOCUS       sequence-221992-        9389 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9389)
  AUTHORS   Mak AB, Ni Z, Hewel JA, Chen GI, Zhong G, Karamboulas K, Blakely K, 
            Smiley S, Marcon E, Roudeva D, Li J, Olsen JB, Punna T, Isserlin R, 
            Chetyrkin S, Gingras AC, Emili A, Greenblatt J, Moffat J
  TITLE     A lentiviral-based functional proteomics approach identifies 
            chromatin remodelling complexes important for the induction of 
            pluripotency.
  JOURNAL   Mol Cell Proteomics. 2010 May;9(5):811-23.
  PUBMED    20305087
REFERENCE   2  (bases 1 to 9389)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics."; date: "2010-05"; volume: "9(5)"; pages: "811-23"
COMMENT     Sequence Label: pLD-puro-CcVA
FEATURES             Location/Qualifiers
     source          1..9389
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..46)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(200..788)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(280..299)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(959..1750,1751..1819))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1582..1601
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1820..1924)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1950..2405)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2087..2108)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2299..2318
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2532..2554
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2546..2563
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2547..2563
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2573..2592
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2573..2591
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      2612..2747
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    2787..2908
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2798..2817)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2852..2871
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             2980..3213
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(3341..3940)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3424..3444)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     3921..3940
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(3962..4472)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4025..4043)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     misc_feature    4521..4638
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(4686..4751)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(4764..4784)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(4797..4817)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(4827..4844)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(4857..4880)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             complement(4917..4940)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     protein_bind    5020..5144
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(5185..5490)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(5287..5306)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(5832..6491)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     6406..6425
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(6545..6551,6552..6569,6570..6575))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(6600..6724)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(6755..6779)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(6776..6979)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(6805..6825)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     complement(7268..7288)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     CDS             complement(7586..7630)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7815..8048
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    8541..8666
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             8713..8893
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(8894..9120)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(9148..9168)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9148..9166)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9187..9203)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9187..9203)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9200..9222)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9211..9227
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9235..9241,9242..9259,9260..9265))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9280..9301
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
       61 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
      121 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
      181 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
      241 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
      301 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
      361 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
      421 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
      481 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
      541 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
      601 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
      661 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
      721 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
      781 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
      841 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
      901 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
      961 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     1021 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     1081 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     1141 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     1201 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     1261 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     1321 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     1381 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     1441 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     1501 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     1561 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     1621 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     1681 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     1741 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     1801 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     1861 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     1921 cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc gttaatattt tgttaaaatt
     1981 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
     2041 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
     2101 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     2161 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
     2221 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
     2281 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
     2341 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
     2401 cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
     2461 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
     2521 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga
     2581 ctcactatag ggcgaattgg gtacgtccct aggcctccaa aaaagcctcc tcactacttc
     2641 tggaatagct cagaggccga ggcggcctcg gcctctgcat aaataaaaaa aattagtcag
     2701 ccatggggcg gagaatgggc ggaactgggc ggagttaggg gcgggatggg cggagttagg
     2761 ggcgggatag ctagagccag acatgataag atacattgat gagtttggac aaaccacaac
     2821 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     2881 aaccattata agctgcaata aacaagttcc tctcactctc tgatattcat ttctttgcaa
     2941 gttataaata ctgaataata agatgacatg aactactact gctagagatt ttccacactg
     3001 actaaaaggg tctgagggat ctctagttac cagagtcaca caacagacgg gcacacacta
     3061 cttgaagcac tcaaggcaag ctttattgag gcttaagcag tgggttccct agttagccag
     3121 agagctccca ggctcagatc tggtctaacc agagagaccc agtacaagca aaaagcagat
     3181 cttgtcttcg ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc
     3241 taagatctac agctgccttg taagtcattg gtcttaaagg taccgatgca tggggtcgtg
     3301 cgctcctttc ggtcgggcgc tgcgggtcgt ggggcgggcg tcaggcaccg ggcttgcggg
     3361 tcatgcacca ggtgcgcggt ccttcgggca cctcgacgtc ggcggtgacg gtgaagccga
     3421 gccgctcgta gaaggggagg ttgcggggcg cggaggtctc caggaaggcg ggcaccccgg
     3481 cgcgctcggc cgcctccact ccggggagca cgacggcgct gcccagaccc ttgccctggt
     3541 ggtcgggcga gacgccgacg gtggccagga accacgcggg ctccttgggc cggtgcggcg
     3601 ccaggaggcc ttccatctgt tgctgcgcgg ccagccggga accgctcaac tcggccatgc
     3661 gcgggccgat ctcggcgaac accgcccccg cttcgacgct ctccggcgtg gtccagaccg
     3721 ccaccgcggc gccgtcgtcc gcgacccaca ccttgccgat gtcgagcccg acgcgcgtga
     3781 ggaagagttc ttgcagctcg gtgacccgct cgatgtggcg gtccggatcg acggtgtggc
     3841 gcgtggcggg gtagtcggcg aacgcggcgg cgagggtgcg tacggccctg gggacgtcgt
     3901 cgcgggtggc gaggcgcacc gtgggcttgt actcggtcat ggtaagctcc ggtggatccc
     3961 cctggggaga gaggtcggtg attcggtcaa cgagggagcc gactgccgac gtgcgctccg
     4021 gaggcttgca gaatgcggaa caccgcgcgg gcaggaacag ggcccacact accgccccac
     4081 accccgcctc ccgcaccgcc ccttcccggc cgctgctctc ggcgcgccct gctgagcagc
     4141 cgctattggc cacagcccat cgcggtcggc gcgctgccat tgctccctgg cgctgtccgt
     4201 ctgcgagggt actagtgaga cgtgcggctt ccgtttgtca cgtccggcac gccgcgaacc
     4261 gcaaggaacc ttcccgactt aggggcggag caggaagcgt cgccgggggg cccacaaggg
     4321 tagcggcgaa gatccgggtg acgctgcgaa cggacgtgaa gaatgtgcga gacccagggt
     4381 cggcgccgct gcgtttcccg gaaccacgcc cagagcagcc gcgtccctgc gcaaacccag
     4441 ggctgccttg gaaaaggcgc aaccccaacc ccctcgagcc gcggccaaag tggatctctg
     4501 ctgtccctgt aataaacccg aaaattttga atttttgtaa tttgtttttg taattcttta
     4561 gtttgtatgt ctgttgctat tatgtctact attctttccc ctgcactgta ccccccaatc
     4621 cccccttttc ttttaaaatt gtggatgaat actgccattt gtctcgaggt cgagaattcg
     4681 aactacttgt catcgtcatc cttgtaatcg atgtcatgat ctttataatc accgtcatgg
     4741 tctttgtagt ctcttcctgc aggcccctga aagtacaggt tctcgctgcc tcctctcccc
     4801 tggaagtaca gattttctcc tgcagggtga tggtgatggt gatggccgct tccgcctttc
     4861 tcgaactgcg ggtggctcca gcttcctcct ccgctgcctc cgccgcttcc tcctcccttc
     4921 tcgaactgag ggtggctcca ggcgatcgcc ttgatcacca cggggggctg gttctcgccc
     4981 agcaggttga acagctctct ggcgatcgct ctagaatcaa ccactttgta caagaaagct
     5041 gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa
     5101 cagactacat aatactgtaa aacacaacat atccagtcac tatggtcgac ctgcagactg
     5161 gctgtgtata agggagcctg acatttatat tccccagaac atcaggttaa tggcgttttt
     5221 gatgtcattt tcgcggtggc tgagatcagc cacttcttcc ccgataacgg agaccggcac
     5281 actggccata tcggtggtca tcatgcgcca gctttcatcc ccgatatgca ccaccgggta
     5341 aagttcacgg gagactttat ctgacagcag acgtgcactg gccaggggga tcaccatccg
     5401 tcgcccgggc gtgtcaataa tatcactctg tacatccaca aacagacgat aacggctctc
     5461 tcttttatag gtgtaaacct taaactgcat ttcaccagcc cctgttctcg tcagcaaaag
     5521 agccgttcat ttcaataaac cgggcgacct cagccatccc ttcctgattt tccgctttcc
     5581 agcgttcggc acgcagacga cgggcttcat tctgcatggt tgtgcttacc agaccggaga
     5641 tattgacatc atatatgcct tgagcaactg atagctgtcg ctgtcaactg tcactgtaat
     5701 acgctgcttc atagcatacc tctttttgac atacttcggg tatacatatc agtatatatt
     5761 cttataccgc aaaaatcagc gcgcaaatac gcatactgtt atctggcttt tagtaagccg
     5821 gatccagatc tttacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc
     5881 attctgccga catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc
     5941 agcaccttgt cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg
     6001 tccatattgg ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgagacg
     6061 aaaaacatat tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc
     6121 acatcttgcg aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc
     6181 gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat
     6241 atcaccagct caccgtcttt cattgccata cggaattccg gatgagcatt catcaggcgg
     6301 gcaagaatgt gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa
     6361 aaggccgtaa tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat
     6421 gcctcaaaat gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt
     6481 tttttctcca ttttagcttc cttagctcct gaaaatctcg acggatccta actcaaaatc
     6541 cacacattat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgcggccgcc
     6601 atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct
     6661 aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttt ttgtacaaac
     6721 ttgtacgcgt acagcgtgga tggcgtctcc aggcgatctg acggttcact aaacgagctc
     6781 tgcttatata gacctcccac cgtacacgcc taccgcccat ttgcgtcaat ggggcggagt
     6841 tgttacgaca ttttggaaag tcccgttgat tttggtgcca aaacaaactc ccattgacgt
     6901 caatggggtg gagacttgga aatccccgtg agtcaaaccg ctatccacgc ccattgatgt
     6961 actgccaaaa ccgcatcacc atggtaatag cgatgactaa tacgtagatg tactgccaag
     7021 taggaaagtc ccataaggtc atgtactggg cataatgcca ggcgggccat ttaccgtcat
     7081 tgacgtcaat agggggcgta cttggcatat gatagtccat tttaaaacat aattttaaaa
     7141 ctgcaaacta cccaagaaat tattactttc tacgtcacgt attttgtact aatatctttg
     7201 tgtttacagt caaattaatt ccaattatct ctctaacagc cttgtatcgt atatgcaaat
     7261 atgaaggaat catgggaaat aggccctcgg tgaagggggc ggccgctcga ggctagtctc
     7321 gtgatcgata ccgtcgagat ccgttcacta atcgaatgga tctgtctctg tctctctctc
     7381 caccttcttc ttctattcct tcgggcctgt cgggtcccct cggggttggg aggtgggtct
     7441 gaaacgataa tggtgaatat ccctgcctaa ctctattcac tatagaaagt acagcaaaaa
     7501 ctattcttaa acctaccaag cctcctacta tcattatgaa taattttata taccacagcc
     7561 aatttgttat gttaaaccaa ttccacaaac ttgcccattt atctaattcc aataattctt
     7621 gttcattctt ttcttgctgg ttttgcgatt cttcaattaa ggagtgtatt aagcttgtgt
     7681 aattgttaat ttctctgtcc cactccatcc aggtcgtgtg attccaaatc tgttccagag
     7741 atttattact ccaactagca ttccaaggca cagcagtggt gcaaatgagt tttccagagc
     7801 aaccccaaat ccccaggagc tgttgatcct ttaggtatct ttccacagcc aggattcttg
     7861 cctggagctg cttgatgccc cagactgtga gttgcaacag atgctgttgc gcctcaatag
     7921 ccctcagcaa attgttctgc tgctgcacta taccagacaa taattgtctg gcctgtaccg
     7981 tcagcgtcat tgacgctgcg cccatagtgc ttcctgctgc tcccaagaac ccaaggaaca
     8041 aagctcctat tcccactgct cttttttctc tctgcaccac tcttctcttt gccttggtgg
     8101 gtgctactcc taatggttca atttttacta ctttatattt atataattca cttctccaat
     8161 tgtccctcat atctcctcct ccaggtctga agatcagcgg ccgcttgctg tgcggtggtc
     8221 ttacttttgt tttgctcttc ctctatcttg tctaaagctt ccttggtgtc ttttatctct
     8281 atcctttgat gcacacaata gagggttgct actgtattat ataatgatct aagttcttct
     8341 gatcctgtct gaagggatgg ttgtagctgt cccagtattt gtctacagcc ttctgatgtt
     8401 tctaacaggc caggattaac tgcgaatcgt tctagctccc tgcttgccca tactatatgt
     8461 tttaatttat attttttctt tccccctggc cttaaccgaa ttttttccca tcgcgatcta
     8521 attctccccc gcttaatact gacgctctcg cacccatctc tctccttcta gcctccgcta
     8581 gtcaaaattt ttggcgtact caccagtcgc cgcccctcgc ctcttgccgt gcgcgcttca
     8641 gcaagccgag tcctgcgtcg agagagctcc tctggtttcc ctttcgcttt caagtccctg
     8701 ttcgggcgcc actgctagag attttccaca ctgactaaaa gggtctgagg gatctctagt
     8761 taccagagtc acacaacaga cgggcacaca ctacttgaag cactcaaggc aagctttatt
     8821 gaggcttaag cagtgggttc cctagttagc cagagagctc ccaggctcag atctggtcta
     8881 accagagaga cccgtttatg tatcgagcta ggcacttaaa tacaatatct ctgcaatgcg
     8941 gcaattcagt ggttcgtcca atccatgtca gacccgtctg ttgccttcct aataaggcac
     9001 gatcgtacca ccttacttcc accaatcggc atgcacggtg ctttttctct ccttgtaagg
     9061 catgttgcta actcatcgtt accatgttgc aagactacaa gagtattgca taagactaca
     9121 ttaagcttgc agctccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta
     9181 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     9241 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     9301 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     9361 atgaatcggc caacgcgcgg ggagaggcg
//
