LOCUS       sequence-222009-        9388 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty sgRNA expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9388)
  AUTHORS   Kurata JS, Lin RJ
  TITLE     MicroRNA-focused CRISPR-Cas9 Library Screen Reveals 
            Fitness-Associated miRNAs.
  JOURNAL   RNA. 2018 May 2. pii: rna.066282.118. doi: 10.1261/rna.066282.118.
  PUBMED    29720387
REFERENCE   2  (bases 1 to 9388)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2018 
            May 2. pii: rna.066282.118. doi: 10.1261/rna.066282.118."
COMMENT     Sequence Label: pLX-sgRNA-BfuAI-2k
FEATURES             Location/Qualifiers
     source          1..9388
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1714..2214
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2133..2151
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2226..2621
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    2681..2798
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2916..3164
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2916..2936
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     3087..3106
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        5096..5171
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(5183..5207)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5245..5833
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5298..5318)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5716..5727)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5905..6138
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6216..6337
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6253..6272)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6307..6326
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6377..6512
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6532..6551)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6533..6551)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6561..6578)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6561..6577)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6570..6592)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6719..7174
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6806..6825)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7016..7037
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7200..7304
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7305..7373,7374..8165)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7523..7542)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8336..8924
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8825..8844
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9078..9095
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9212..9233
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9248..9253,9254..9271,9272..9278)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9286..9302
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9291..9313
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9310..9326
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9310..9326
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9345..9365
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9347..9365
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccttgcgcc ttttccaagg cagccctggg
     1741 tttgcgcagg gacgcggctg ctctgggcgt ggttccggga aacgcagcgg cgccgaccct
     1801 gggtctcgca cattcttcac gtccgttcgc agcgtcaccc ggatcttcgc cgctaccctt
     1861 gtgggccccc cggcgacgct tcctgctccg cccctaagtc gggaaggttc cttgcggttc
     1921 gcggcgtgcc ggacgtgaca aacggaagcc gcacgtctca ctagtaccct cgcagacgga
     1981 cagcgccagg gagcaatggc agcgcgccga ccgcgatggg ctgtggccaa tagcggctgc
     2041 tcagcagggc gcgccgagag cagcggccgg gaaggggcgg tgcgggaggc ggggtgtggg
     2101 gcggtagtgt gggccctgtt cctgcccgcg cggtgttccg cattctgcaa gcctccggag
     2161 cgcacgtcgg cagtcggctc cctcgttgac cgaatcaccg acctctctcc ccagggggta
     2221 ccaccatggc caagcctttg tctcaagaag aatccaccct cattgaaaga gcaacggcta
     2281 caatcaacag catccccatc tctgaagact acagcgtcgc cagcgcagct ctctctagcg
     2341 acggccgcat cttcactggt gtcaatgtat atcattttac tgggggacct tgtgcagaac
     2401 tcgtggtgct gggcactgct gctgctgcgg cagctggcaa cctgacttgt atcgtcgcga
     2461 tcggaaatga gaacaggggc atcttgagcc cctgcggacg gtgccgacag gtgcttctcg
     2521 atctgcatcc tgggatcaaa gccatagtga aggacagtga tggacagccg acggcagttg
     2581 ggattcgtga attgctgccc tctggttatg tgtgggaggg cctgcagctg cagtagtaag
     2641 aattctagat cttgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     2701 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     2761 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     2821 gagatccact ttggcgccgg ctcgagtgta caaaaaagca ggctttaaag gaaccaattc
     2881 agtcgactgg atccggtacc aaggtcgggc aggaagaggg cctatttccc atgattcctt
     2941 catatttgca tatacgatac aaggctgtta gagagataat tagaattaat ttgactgtaa
     3001 acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt gggtagtttg
     3061 cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact tgaaagtatt
     3121 tcgatttctt ggctttatat atcttgtgga aaggacgaaa caccctttgc aggttccacc
     3181 acttgttcga gacggttgta aatgagcaca caaaatacac atgctaaaat attatattct
     3241 atgaccttta taaaatcaac caaaatcttc tttttaataa ctttagtatc aataattaga
     3301 atttttatgt tcctttttgc aaacttttaa taaaaatgag caaaataaaa aaacgctagt
     3361 tttagtaact cgcgttgttt tcttcacctt taataatagc tactccacca cttgttccta
     3421 agcggtcagc tcctgcttca atcatttttt gagcatcttc aaatgttcta actccaccag
     3481 ctgctttaac taaagcattg tctttaacaa ctgacttcat tagtttaaca tcttcaaatg
     3541 ttgcacctga ttttgaaaat cctgttgatg ttttaacaaa ttctaatcca gcttcaacag
     3601 ctatttcaca agctttcatg atttcttctt ttgttaataa acaattttcc ataatacatt
     3661 taacaacatg tgatccagct gcttttttta cagctttcat gtcttctaaa actaattcat
     3721 aatttttgtc ttttaatgca ccaatattta ataccatatc aatttctgtt gcaccatctt
     3781 taattgcttc agaaacttcg aatgcttttg tagctgttgt gcatgcacct agaggaaaac
     3841 ctacaacatt tgttattcct acatttgtgc cttttaataa ttctttacaa tagcttgttc
     3901 aatatgaatt aacacaaact gttgcaaaat caaattcaat tgcttcatca cataattgtt
     3961 taatttcagc tttcgtagca tcttgtttta ataatgtgtg atctatatat ttgtttagtt
     4021 tcattttttc tcctatatat tcatttttaa ttttaattct ttaataattt cgtctacttt
     4081 aactttagcg ttttgaacag attcaccaac acctataaaa taaattttta gtttaggttc
     4141 agttccactt gggcgaacag caaatcatga cttatcttct aaataaaatt ttagtaagtc
     4201 ttgtcctggc atattataca ttccatcgat gtagtcttca acattaacaa ctttaagtcc
     4261 agcaatttga gttaagggtg ttgctctcaa tgatttcatt aatggttcaa tttttaattt
     4321 cttttcttct ggtttaaaat tcaagtttaa agtgaaagtg taatatgcac ccatttcttt
     4381 aaataaatct tctaaatagt ctactaatgt tttattttgt tttttataaa atcaagcagc
     4441 ctctgctatt aatatagaag cttgtattcc atctttatct ctagctgagt catcaattac
     4501 atatccataa ctttcttcat aagcaaaaac aaaatttaat ccgttatctt cttctttagc
     4561 aatttctcta cccattcatt taaatccagt taaagttttt acaatattaa ctccatattt
     4621 ttcatgagcg attctatcac ccaaatcact tgttacaaaa cttgaatata gagccggatt
     4681 ttttggaatg ctatttaagc gttttagatt tgataatttt caatcaatta aaattggtcc
     4741 tgtttgattt ccatctaatc ttacaaaatg accatcatgt tttattgcca ttccaaatct
     4801 gtcagcatct gggtcattca taataataat atctgcatca tgtttaatac catattcaag
     4861 cggtattttt catgcaggat caaattctgg atttggattt acaacatttt taaatgtttc
     4921 atcttcaaat gcatgctctt caacctcaat aacgttatat cctgattcac gtaatatttt
     4981 tggggtaaat ttagttcctg ttccattaac tgcgctaaaa ataattttta aatctttttt
     5041 agcttcttgc tcttttttgt acgtctctca gctcctgctt caatcacctg cggtcgtttt
     5101 agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac
     5161 cgagtcggtg ctttttttct agacccagct ttcttgtaca aagttggcat tagctagcgc
     5221 taaccggtgg cgcgttaagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg
     5281 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     5341 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     5401 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     5461 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     5521 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     5581 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     5641 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     5701 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     5761 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     5821 gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     5881 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     5941 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     6001 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     6061 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     6121 tgtggaaaat ctctagcagt acgtatagta gttcatgtca tcttattatt cagtatttat
     6181 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg
     6241 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     6301 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc
     6361 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     6421 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     6481 ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt
     6541 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     6601 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     6661 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
     6721 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     6781 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     6841 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     6901 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     6961 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     7021 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     7081 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     7141 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
     7201 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     7261 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     7321 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     7381 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     7441 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     7501 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     7561 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     7621 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     7681 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     7741 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     7801 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     7861 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     7921 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     7981 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     8041 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     8101 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     8161 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     8221 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     8281 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     8341 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     8401 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     8461 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     8521 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     8581 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     8641 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     8701 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     8761 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     8821 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     8881 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     8941 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     9001 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     9061 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     9121 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     9181 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     9241 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     9301 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
     9361 aaagggaaca aaagctggag ctgcaagc
//
