LOCUS       sequence-222011-        4390 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  firefly luciferase (Fluc) reporter containing the 5'-UTR of PACT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4390)
  AUTHORS   Lai MC, Sun HS, Wang SW, Tarn WY
  TITLE     DDX3 functions in antiviral innate immunity through translational 
            control of PACT.
  JOURNAL   FEBS J. 2016 Jan;283(1):88-101. doi: 10.1111/febs.13553. Epub 2015 
            Oct 31.
  PUBMED    26454002
REFERENCE   2  (bases 1 to 4390)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/febs.13553"; journalName: "FEBS J"; date: "2016-01"; 
            volume: "283"; issue: "1"; pages: "88-101"
COMMENT     Sequence Label: pFL-SV40-PACT 5'-UTR
FEATURES             Location/Qualifiers
     source          1..4390
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        62..419
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        62..253
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(85..105)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      270..405
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      656..665
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             662..2314
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(728..746)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    2345..2466
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2356..2375)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2410..2429
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        2599..2703
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2704..2772,2773..3564)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2922..2941)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3735..4323
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4224..4243
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agatctgcgc agcaccatgg cctgaaataa cctctgaaag aggaacttgg ttaggtacct
       61 tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
      121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
      181 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
      241 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
      301 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
      361 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
      421 agcttgcgag ggggcgtagc cggagctacg gcaccaaggc tccgccccca ccctgcctgc
      481 cccctcgctg gagcaacgca agcaggaggc gggggagtcg gaggaggtgg cggcgctgga
      541 gctcctcccg gggaccagcg acccggggag cgagcacgtc gctccgcacc gctcttcctc
      601 cagccgctga gccgtccctt ctcgccaagc ttggcattcc ggtactgttg gtaaagccac
      661 catggaagac gccaaaaaca taaagaaagg cccggcgcca ttctatccgc tggaagatgg
      721 aaccgctgga gagcaactgc ataaggctat gaagagatac gccctggttc ctggaacaat
      781 tgcttttaca gatgcacata tcgaggtgga catcacttac gctgagtact tcgaaatgtc
      841 cgttcggttg gcagaagcta tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt
      901 atgcagtgaa aactctcttc aattctttat gccggtgttg ggcgcgttat ttatcggagt
      961 tgcagttgcg cccgcgaacg acatttataa tgaacgtgaa ttgctcaaca gtatgggcat
     1021 ttcgcagcct accgtggtgt tcgtttccaa aaaggggttg caaaaaattt tgaacgtgca
     1081 aaaaaagctc ccaatcatcc aaaaaattat tatcatggat tctaaaacgg attaccaggg
     1141 atttcagtcg atgtacacgt tcgtcacatc tcatctacct cccggtttta atgaatacga
     1201 ttttgtgcca gagtccttcg atagggacaa gacaattgca ctgatcatga actcctctgg
     1261 atctactggt ctgcctaaag gtgtcgctct gcctcataga actgcctgcg tgagattctc
     1321 gcatgccaga gatcctattt ttggcaatca aatcattccg gatactgcga ttttaagtgt
     1381 tgttccattc catcacggtt ttggaatgtt tactacactc ggatatttga tatgtggatt
     1441 tcgagtcgtc ttaatgtata gatttgaaga agagctgttt ctgaggagcc ttcaggatta
     1501 caagattcaa agtgcgctgc tggtgccaac cctattctcc ttcttcgcca aaagcactct
     1561 gattgacaaa tacgatttat ctaatttaca cgaaattgct tctggtggcg ctcccctctc
     1621 taaggaagtc ggggaagcgg ttgccaagag gttccatctg ccaggtatca ggcaaggata
     1681 tgggctcact gagactacat cagctattct gattacaccc gagggggatg ataaaccggg
     1741 cgcggtcggt aaagttgttc cattttttga agcgaaggtt gtggatctgg ataccgggaa
     1801 aacgctgggc gttaatcaaa gaggcgaact gtgtgtgaga ggtcctatga ttatgtccgg
     1861 ttatgtaaac aatccggaag cgaccaacgc cttgattgac aaggatggat ggctacattc
     1921 tggagacata gcttactggg acgaagacga acacttcttc atcgttgacc gcctgaagtc
     1981 tctgattaag tacaaaggct atcaggtggc tcccgctgaa ttggaatcca tcttgctcca
     2041 acaccccaac atcttcgacg caggtgtcgc aggtcttccc gacgatgacg ccggtgaact
     2101 tcccgccgcc gttgttgttt tggagcacgg aaagacgatg acggaaaaag agatcgtgga
     2161 ttacgtcgcc agtcaagtaa caaccgcgaa aaagttgcgc ggaggagttg tgtttgtgga
     2221 cgaagtaccg aaaggtctta ccggaaaact cgacgcaaga aaaatcagag agatcctcat
     2281 aaaggccaag aagggcggaa agatcgccgt gtaattctag agcggccgct tcgagcagac
     2341 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
     2401 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
     2461 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag
     2521 gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga tccaggtggc
     2581 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     2641 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     2701 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     2761 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     2821 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     2881 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     2941 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     3001 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     3061 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     3121 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     3181 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     3241 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     3301 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     3361 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     3421 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     3481 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     3541 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     3601 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     3661 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     3721 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     3781 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     3841 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     3901 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     3961 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     4021 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     4081 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     4141 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     4201 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     4261 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     4321 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     4381 atggctcgac
//
