LOCUS       sequence-222021-       11237 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  H2BVenus_p2A_HygroR_p2A_CreERt2 targeting plasmid with cloning sites
            ready for homology arms.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11237)
  AUTHORS   Hartman BH, Bscke R, Ellwanger DC, Keymeulen S, Scheibinger M, 
            Heller S
  TITLE     Fbxo2(VHC) mouse and embryonic stem cell reporter lines delineate in
            vitro-generated inner ear sensory epithelia cells and enable otic 
            lineage selection and Cre-recombination.
  JOURNAL   Dev Biol. 2018 Sep 1. pii: S0012-1606(18)30499-8. doi: 
            10.1016/j.ydbio.2018.08.013.
  PUBMED    30179592
REFERENCE   2  (bases 1 to 11237)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Biol. 
            2018 Sep 1. pii: S0012-1606(18)30499-8. doi: 
            10.1016/j.ydbio.2018.08.013."
COMMENT     Sequence Label: pVHC_PGKneoLox2DTA.2
FEATURES             Location/Qualifiers
     source          1..11237
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      4..459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(91..110)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     301..322
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             734..1111
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAVTKYTSAK"
     CDS             join(1118..1120,1121..1123,1124..1834)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1424..1443)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1771..1792
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1844..1900
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             1901..2923
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             1901..2923
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             2936..2992
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2993..4021
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             4034..4969
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             4082..4975
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATA"
     primer_bind     complement(5063..5082)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5128..5183
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5182..5201)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5544..5560)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5568..5584
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5607..5623
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5607..5623
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    5637..5670
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        5700..6199
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             6213..7016
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6276..6295)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6886..6905
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7054..7278
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    7337..7370
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        7449..7948
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             7975..8547
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphtheria toxin fragment A"
                     /label=DTA
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
     intron          8624..8689
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    8763..8987
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(9049..9069)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9049..9067)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9088..9104)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9112..9128
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9136..9142,9143..9160,9161..9166))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9181..9202
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9319..9336)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9490..10078)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9570..9589)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10249..11040,11041..11109))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10872..10891
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11110..11214)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
      361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
      661 ccgcggtggc ggccgcctag gagatctacg cgtcaattga tgcatcccgg gtttattcat
      721 gaggccggcc accatgccag agccagcgaa gtctgctccc gccccgaaaa agggctccaa
      781 gaaggcggtg actaaggcgc agaagaaaga cggcaagaag cgcaagcgca gccgcaagga
      841 gagctattcc atctatgtgt acaaggttct gaagcaggtc caccctgaca ccggcatttc
      901 gtccaaggcc atgggcatca tgaattcgtt tgtgaacgac attttcgagc gcatcgcagg
      961 tgaggcttcc cgcctggcgc attacaacaa gcgctcgacc atcacctcca gggagatcca
     1021 gacggccgtg cgcctgctgc tgcctgggga gttggccaag cacgccgtgt ccgagggtac
     1081 taaggccgtc accaagtaca ccagcgctaa atccaccatg gtgagcaagg gcgaggagct
     1141 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     1201 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagctgat
     1261 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgggctacgg
     1321 cctgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     1381 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     1441 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     1501 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     1561 ccacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat
     1621 ccgccacaac atcgaggacg gcggcgtgca gctcgccgac cactaccagc agaacacccc
     1681 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccgccct
     1741 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     1801 tgggatcact ctcggcatgg acgagctgta caagggaagc ggagctacta acttcagcct
     1861 gctgaagcag gctggagacg tggaggagaa ccctggacct atgaaaaagc ctgaactcac
     1921 cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca
     1981 gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt
     2041 cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt
     2101 tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattca gcgagagcct
     2161 gacctattgc atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga
     2221 actgcccgct gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct
     2281 tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg
     2341 gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga
     2401 cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga
     2461 ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga
     2521 caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata
     2581 cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg
     2641 ctacttcgag cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct
     2701 ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc
     2761 ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac
     2821 acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga
     2881 tagtggaaac cgacgcccca gcactcgtcc gagggcaaag gaacgcggct ccggcgcaac
     2941 aaatttctct ctcctcaaac aagcaggcga tgttgaagaa aatcccgggc ccatgtccaa
     3001 tttactgacc gtacaccaaa atttgcctgc attaccggtc gatgcaacga gtgatgaggt
     3061 tcgcaagaac ctgatggaca tgttcaggga tcgccaggcg ttttctgagc atacctggaa
     3121 aatgcttctg tccgtttgcc ggtcgtgggc ggcatggtgc aagttgaata accggaaatg
     3181 gtttcccgca gaacctgaag atgttcgcga ttatcttcta tatcttcagg cgcgcggtct
     3241 ggcagtaaaa actatccagc aacatttggg ccagctaaac atgcttcatc gtcggtccgg
     3301 gctgccacga ccaagtgaca gcaatgctgt ttcactggtt atgcggcgga tccgaaaaga
     3361 aaacgttgat gccggtgaac gtgcaaaaca ggctctagcg ttcgaacgca ctgatttcga
     3421 ccaggttcgt tcactcatgg aaaatagcga tcgctgccag gatatacgta atctggcatt
     3481 tctggggatt gcttataaca ccctgttacg tatagccgaa attgccagga tcagggttaa
     3541 agatatctca cgtactgacg gtgggagaat gttaatccat attggcagaa cgaaaacgct
     3601 ggttagcacc gcaggtgtag agaaggcact tagcctgggg gtaactaaac tggtcgagcg
     3661 atggatttcc gtctctggtg tagctgatga tccgaataac tacctgtttt gccgggtcag
     3721 aaaaaatggt gttgccgcgc catctgccac cagccagcta tcaactcgcg ccctggaagg
     3781 gatttttgaa gcaactcatc gattgattta cggcgctaag gatgactctg gtcagagata
     3841 cctggcctgg tctggacaca gtgcccgtgt cggagccgcg cgagatatgg cccgcgctgg
     3901 agtttcaata ccggagatca tgcaagctgg tggctggacc aatgtaaata ttgtcatgaa
     3961 ctatatccgt aacctggata gtgaaacagg ggcaatggtg cgcctgctgg aagatggcga
     4021 tctcgagcca tctgctggag acatgagagc tgccaacctt tggccaagcc cgctcatgat
     4081 caaacgctct aagaagaaca gcctggcctt gtccctgacg gccgaccaga tggtcagtgc
     4141 cttgttggat gctgagcccc ccatactcta ttccgagtat gatcctacca gacccttcag
     4201 tgaagcttcg atgatgggct tactgaccaa cctggcagac agggagctgg ttcacatgat
     4261 caactgggcg aagagggtgc caggctttgt ggatttgacc ctccatgatc aggtccacct
     4321 tctagaatgt gcctggctag agatcctgat gattggtctc gtctggcgct ccatggagca
     4381 cccagtgaag ctactgtttg ctcctaactt gctcttggac aggaaccagg gaaaatgtgt
     4441 agagggcatg gtggagatct tcgacatgct gctggctaca tcatctcggt tccgcatgat
     4501 gaatctgcag ggagaggagt ttgtgtgcct caaatctatt attttgctta attctggagt
     4561 gtacacattt ctgtccagca ccctgaagtc tctggaagag aaggaccata tccaccgagt
     4621 cctggacaag atcacagaca ctttgatcca cctgatggcc aaggcaggcc tgaccctgca
     4681 gcagcagcac cagcggctgg cccagctcct cctcatcctc tcccacatca ggcacatgag
     4741 taacaaaggc atggagcatc tgtacagcat gaagtgcaag aacgtggtgc ccctctatga
     4801 cctgctgctg gaggcggcgg acgcccaccg cctacatgcg cccactagcc gtggaggggc
     4861 atccgtggag gagacggacc aaagccactt ggccactgcg ggctctactt catcgcattc
     4921 cttgcaaaag tattacatca cgggggaggc agagggtttc cctgccacag cttgatagcg
     4981 gccgcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg gccaatgccc
     5041 tggctcacaa ataccactga gatctttttc cctctgccaa aaattatggg gacatcatga
     5101 agccccttga gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt
     5161 gttggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac
     5221 atcagaatga gtatttggtt tagagtttgg caacatatgc ccatatgctg gctgccatga
     5281 acaaaggttg gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct
     5341 tattccatag aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat
     5401 ttttttcttt aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc
     5461 tctcctgact actcccagtc atagctgtcc ctcttctctt atggagatcc ctcgacctgc
     5521 agcccaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     5581 acaattccac acaaaaacta ggccgctcta gaactagtgg atccggaacc cttaatataa
     5641 cttcgtataa tgtatgctat acgaagttat taggtccctc gacctgcagg aattctaccg
     5701 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg
     5761 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg
     5821 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag
     5881 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg
     5941 tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg
     6001 gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg
     6061 tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc
     6121 cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc
     6181 ctcatctccg ggcctttcga cctgcagcca atatgggatc ggccattgaa caagatggat
     6241 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     6301 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     6361 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc
     6421 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     6481 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     6541 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     6601 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     6661 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     6721 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgatgat ctcgtcgtga
     6781 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     6841 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     6901 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     6961 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagggg
     7021 atcaattctc tagagctcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     7081 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     7141 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     7201 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     7261 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctcg aatcaagctg
     7321 atccggaacc cttaatataa cttcgtataa tgtatgctat acgaagttat taggtccctc
     7381 gacctgcagc ccaagctagc ttatcgatac cgtcgacggt atcgataagc ttgatatcga
     7441 attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc
     7501 cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac
     7561 cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc
     7621 cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga
     7681 agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt
     7741 aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct
     7801 gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg
     7861 aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt
     7921 ctcctcttcc tcatctccgg gcctttcgac ctgcaggtcc tcgccatgga tcctgatgat
     7981 gttgttgatt cttctaaatc ttttgtgatg gaaaactttt cttcgtacca cgggactaaa
     8041 cctggttatg tagattccat tcaaaaaggt atacaaaagc caaaatctgg tacacaagga
     8101 aattatgacg atgattggaa agggttttat agtaccgaca ataaatacga cgctgcggga
     8161 tactctgtag ataatgaaaa cccgctctct ggaaaagctg gaggcgtggt caaagtgacg
     8221 tatccaggac tgacgaaggt tctcgcacta aaagtggata atgccgaaac tattaagaaa
     8281 gagttaggtt taagtctcac tgaaccgttg atggagcaag tcggaacgga agagtttatc
     8341 aaaaggttcg gtgatggtgc ttcgcgtgta gtgctcagcc ttcccttcgc tgaggggagt
     8401 tctagcgttg aatatattaa taactgggaa caggcgaaag cgttaagcgt agaacttgag
     8461 attaattttg aaacccgtgg aaaacgtggc caagatgcga tgtatgagta tatggctcaa
     8521 gcctgtgcag gaaatcgtgt caggcgatct ctttgtgaag gaaccttact tctgtggtgt
     8581 gacataattg gacaaactac ctacagagat ttaaagctct aaggtaaata taaaattttt
     8641 aagtgtataa tgtgttaaac tactgattct aattgtttgt gtattttaga ttccaaccta
     8701 tggaactgat gaatgggagc agtggtggaa tgcagatcct agagctcgct gatcagcctc
     8761 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     8821 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     8881 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     8941 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     9001 aagaaccagc tggggctcga gggggggccc ggtacccagc ttttgttccc tttagtgagg
     9061 gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     9121 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     9181 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     9241 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     9301 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     9361 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     9421 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     9481 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     9541 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     9601 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     9661 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     9721 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     9781 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     9841 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     9901 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     9961 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
    10021 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
    10081 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
    10141 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
    10201 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
    10261 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
    10321 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
    10381 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
    10441 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
    10501 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
    10561 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
    10621 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
    10681 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
    10741 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    10801 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    10861 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    10921 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    10981 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    11041 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    11101 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    11161 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    11221 tccccgaaaa gtgccac
//
