LOCUS       sequence-222022-        4430 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  firefly luciferase (Fluc) reporter containing the 5'-UTR of Rac1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Ku YC, Lai MH, Lo CC, Cheng YC, Qiu JT, Tarn WY, Lai MC
  TITLE     DDX3 Participates in Translational Control of Inflammation Induced 
            by Infections and Injuries.
  JOURNAL   Mol Cell Biol. 2018 Dec 11;39(1). pii: MCB.00285-18. doi: 
            10.1128/MCB.00285-18. Print 2019 Jan 1.
  PUBMED    30373933
REFERENCE   2  (bases 1 to 4430)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "2018-12-11"; volume: "39(1). pii: MCB.00285-18. doi";
            pages: " 10.1128/MCB.00285-18. Print 2019 Jan 1"
COMMENT     Sequence Label: pFL-SV40-Rac1 5'-UTR
FEATURES             Location/Qualifiers
     source          1..4430
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        62..419
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        62..253
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(85..105)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      270..405
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      696..705
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             702..2354
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(768..786)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    2385..2506
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2396..2415)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2450..2469
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        2639..2743
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2744..2812,2813..3604)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2962..2981)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3775..4363
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4264..4283
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agatctgcgc agcaccatgg cctgaaataa cctctgaaag aggaacttgg ttaggtacct
       61 tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
      121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
      181 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
      241 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
      301 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
      361 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
      421 agcttgggag gccggatgtg agtggagcgg ccatttcctg tttctctgca gttttcctca
      481 gctttgggtg gtggccgctg ccgggcatcg gcttccagtc cgcggagggc gaggcggcgt
      541 ggacagcggc cccggcaccc agcgccccgc cgcccgcaag ccgcgcgccc gtccgccgcg
      601 ccccgagccc gccgcttcct atctcagcgc cctgccgccg ccgccgcggc ccagcgagcg
      661 gccctgaagc ttggcattcc ggtactgttg gtaaagccac catggaagac gccaaaaaca
      721 taaagaaagg cccggcgcca ttctatccgc tggaagatgg aaccgctgga gagcaactgc
      781 ataaggctat gaagagatac gccctggttc ctggaacaat tgcttttaca gatgcacata
      841 tcgaggtgga catcacttac gctgagtact tcgaaatgtc cgttcggttg gcagaagcta
      901 tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt atgcagtgaa aactctcttc
      961 aattctttat gccggtgttg ggcgcgttat ttatcggagt tgcagttgcg cccgcgaacg
     1021 acatttataa tgaacgtgaa ttgctcaaca gtatgggcat ttcgcagcct accgtggtgt
     1081 tcgtttccaa aaaggggttg caaaaaattt tgaacgtgca aaaaaagctc ccaatcatcc
     1141 aaaaaattat tatcatggat tctaaaacgg attaccaggg atttcagtcg atgtacacgt
     1201 tcgtcacatc tcatctacct cccggtttta atgaatacga ttttgtgcca gagtccttcg
     1261 atagggacaa gacaattgca ctgatcatga actcctctgg atctactggt ctgcctaaag
     1321 gtgtcgctct gcctcataga actgcctgcg tgagattctc gcatgccaga gatcctattt
     1381 ttggcaatca aatcattccg gatactgcga ttttaagtgt tgttccattc catcacggtt
     1441 ttggaatgtt tactacactc ggatatttga tatgtggatt tcgagtcgtc ttaatgtata
     1501 gatttgaaga agagctgttt ctgaggagcc ttcaggatta caagattcaa agtgcgctgc
     1561 tggtgccaac cctattctcc ttcttcgcca aaagcactct gattgacaaa tacgatttat
     1621 ctaatttaca cgaaattgct tctggtggcg ctcccctctc taaggaagtc ggggaagcgg
     1681 ttgccaagag gttccatctg ccaggtatca ggcaaggata tgggctcact gagactacat
     1741 cagctattct gattacaccc gagggggatg ataaaccggg cgcggtcggt aaagttgttc
     1801 cattttttga agcgaaggtt gtggatctgg ataccgggaa aacgctgggc gttaatcaaa
     1861 gaggcgaact gtgtgtgaga ggtcctatga ttatgtccgg ttatgtaaac aatccggaag
     1921 cgaccaacgc cttgattgac aaggatggat ggctacattc tggagacata gcttactggg
     1981 acgaagacga acacttcttc atcgttgacc gcctgaagtc tctgattaag tacaaaggct
     2041 atcaggtggc tcccgctgaa ttggaatcca tcttgctcca acaccccaac atcttcgacg
     2101 caggtgtcgc aggtcttccc gacgatgacg ccggtgaact tcccgccgcc gttgttgttt
     2161 tggagcacgg aaagacgatg acggaaaaag agatcgtgga ttacgtcgcc agtcaagtaa
     2221 caaccgcgaa aaagttgcgc ggaggagttg tgtttgtgga cgaagtaccg aaaggtctta
     2281 ccggaaaact cgacgcaaga aaaatcagag agatcctcat aaaggccaag aagggcggaa
     2341 agatcgccgt gtaattctag agcggccgct tcgagcagac atgataagat acattgatga
     2401 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     2461 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     2521 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
     2581 cctctacaaa tgtggtaaaa tcgataagga tccaggtggc acttttcggg gaaatgtgcg
     2641 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     2701 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     2761 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     2821 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     2881 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     2941 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     3001 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     3061 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     3121 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     3181 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     3241 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     3301 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     3361 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     3421 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     3481 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     3541 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     3601 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     3661 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     3721 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     3781 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     3841 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     3901 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     3961 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     4021 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     4081 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     4141 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     4201 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     4261 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     4321 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     4381 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atggctcgac
//
