LOCUS       sequence-222034-        4141 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multisite gateway vector for 3' tagging with yeast ATG18 (PLEKHA3). 
            Binds PtdIns(3,5P)2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 4141)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: p3E-ATG18
FEATURES             Location/Qualifiers
     source          1..4141
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..125)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1627..1722)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1759..1778)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1760..1778)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1783..1799)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1783..1799)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1901..1920)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1912..2721
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1979..1998)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2868..3456
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3357..3376
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3610..3627
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3786..3813)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3905..3991)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4040..4062
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4054..4071
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4055..4071
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 catagtgact gcatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc
       61 taatttaata tattgatatt tatatcattt tacgtttctc gttcaacttt cttgtacaaa
      121 gtggtgtctg attcatcacc tactatcaac tttattaatt tcaatcaaac cggaacgtgt
      181 atttcccttg gaacgtcgaa aggtttcaaa atattcaatt gtgagccctt cggaaaattt
      241 tattcagagg acagtggggg ctatgctatc gtcgagatgt tgttctccac ctcgttacta
      301 gccctcgttg ggataggcga tcaacctgcg ctttcaccaa ggagattgcg tataatcaac
      361 acaaaaaaac attctattat ctgtgaggtg actttcccta cttctattct gagtgtgaaa
      421 atgaataagt ctcgattggt ggtactttta caagagcaga tttatattta tgatatcaac
      481 accatgagac tattgcatac tatagaaaca aaccctaacc cacgtggcct tatggctatg
      541 tctccttcgg tagccaacag ctatttagtg tatccatcac caccaaaagt tattaactcc
      601 gaaattaaag ctcatgccac cacaaacaat atcacattgt cagttggtgg caacacagag
      661 accagtttca agagagatca gcaagatgct ggccatagtg acattagcga cttggatcag
      721 tattcgagct ttactaagag ggatgatgcg gatccaacaa gcagtaacgg cggtaacagc
      781 agtataataa agaatggtga tgtgatcgta ttcaacttgg aaacattaca gccaaccatg
      841 gtcatcgaag ctcataaggg cgagattgct gcaatggcaa ttagttttga tgggacacta
      901 atggctaccg cctctgataa aggtactatc atcagggtct ttgacattga aacgggtgat
      961 aagatctacc aattcaggag agggacgtac gcgacaagaa tttactccat atcattcagt
     1021 gaagatagcc agtacttggc ggttaccggc tcttccaaaa ccgtgcatat cttcaaattg
     1081 gggcattcaa tgagcaacaa taaactagac agcgatgata gcaacatgga agaagctgca
     1141 gccgatgatt catcgctcga taccaccagt atcgatgcgc tgagtgacga ggaaaacccg
     1201 acaagactcg caagagaacc atatgtggat gcatcaagaa agacaatggg taggatgata
     1261 cgttactctt ctcaaaagct atcccgaaga gctgccagaa cattgggtca gattttcccc
     1321 atcaaagtta catcgttgtt ggaatcctcg cgccattttg cgtctttgaa acttcccgtt
     1381 gaaaccaatt cccatgtaat gaccatatca agtataggct ctccaataga tatagacaca
     1441 tccgagtatc cggaactctt cgaaactggc aattccgcaa gtacagagtc ctaccatgag
     1501 cctgttatga agatggtccc catcagggtc gtttcctcgg atggatacct atacaacttt
     1561 gttatggacc cggagagagg cggcgattgc ttaatattgt cacagtattc catcttgatg
     1621 gattgacaac tttattatac aaagttggca ttataaaaaa gcattgctta tcaatttgtt
     1681 gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttggagctcc atggtagcgt
     1741 taacgcggcc gcgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg
     1801 cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca
     1861 tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat
     1921 attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga tttatatggg
     1981 tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg
     2041 aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt
     2101 acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag
     2161 cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca
     2221 gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca
     2281 gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc
     2341 gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat
     2401 tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt
     2461 ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt
     2521 tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga
     2581 taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa
     2641 cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg
     2701 atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg gcagagcatt
     2761 acgctgactt gacgggacgg cgcaagctca tgaccaaaat cccttaacgt gagttacgcg
     2821 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     2881 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     2941 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     3001 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     3061 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     3121 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     3181 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     3241 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     3301 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     3361 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     3421 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     3481 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     3541 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     3601 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     3661 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     3721 gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa
     3781 acgcaaaaag gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg
     3841 gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg
     3901 gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc
     3961 agtcttccga ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcgtta
     4021 acgctagcat ggatgttttc ccagtcacga cgttgtaaaa cgacggccag tcttaagctc
     4081 gggccctgca gctctagagc tcgaattcta caggtcacta ataccatcta agtagttggt
     4141 t
//
