LOCUS       sequence-222036-       10562 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression plasmid with CAG promoter driving multicistronic cassette
            H2BtdTomato_p2A_HygroR_p2A_CreERt2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10562)
  AUTHORS   Hartman BH, Bscke R, Ellwanger DC, Keymeulen S, Scheibinger M, 
            Heller S
  TITLE     Fbxo2(VHC) mouse and embryonic stem cell reporter lines delineate in
            vitro-generated inner ear sensory epithelia cells and enable otic 
            lineage selection and Cre-recombination.
  JOURNAL   Dev Biol. 2018 Sep 1. pii: S0012-1606(18)30499-8. doi: 
            10.1016/j.ydbio.2018.08.013.
  PUBMED    30179592
REFERENCE   2  (bases 1 to 10562)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Biol. 
            2018 Sep 1. pii: S0012-1606(18)30499-8. doi: 
            10.1016/j.ydbio.2018.08.013."
COMMENT     Sequence Label: pCAG-tdTHC
FEATURES             Location/Qualifiers
     source          1..10562
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             28..405
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAVTKYTSAK"
     CDS             412..1839
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGRYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     CDS             1849..1905
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             1906..2928
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             1906..2928
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             2941..2997
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2998..4026
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             4039..4974
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             4087..4980
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATA"
     primer_bind     complement(5068..5087)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5133..5188
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5187..5206)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5549..5565)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5549..5565)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5562..5584)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5573..5589
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5597..5603,5604..5621,5622..5627))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     rep_origin      complement(5675..6130)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5812..5833)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6024..6043
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6157..6261
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6263..6620
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6263..6454
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6286..6306)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6471..6606
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6655..7449
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6655..7449
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6709..6728)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7319..7338
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7637..7656)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7681..7728
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8057..8645
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8546..8565
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        8819..9198
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9200..9475
                     /label=chicken beta-actin promoter
     intron          9476..10484
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     10408..10430
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     10492..10511
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
ORIGIN
        1 ggcaaagaat tcatgaggcc ggccaccatg ccagagccag cgaagtctgc tcccgccccg
       61 aaaaagggct ccaagaaggc ggtgactaag gcgcagaaga aagacggcaa gaagcgcaag
      121 cgcagccgca aggagagcta ttccatctat gtgtacaagg ttctgaagca ggtccaccct
      181 gacaccggca tttcgtccaa ggccatgggc atcatgaatt cgtttgtgaa cgacattttc
      241 gagcgcatcg caggtgaggc ttcccgcctg gcgcattaca acaagcgctc gaccatcacc
      301 tccagggaga tccagacggc cgtgcgcctg ctgctgcctg gggagttggc caagcacgcc
      361 gtgtccgagg gtactaaggc cgtcaccaag tacaccagcg ctaaatccac catggtgagc
      421 aagggagagg aggtcatcaa agagttcatg cgcttcaagg tgcgcatgga gggctccatg
      481 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
      541 accgccaagc tgaaggtgac caagggcggc cccctgccct tcgcctggga catcctgtcc
      601 ccccagttca tgtacggctc caaggcgtac gtgaagcacc ccgccgacat ccccgattac
      661 aagaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
      721 ggtctggtga ccgtgaccca ggactcctcc ctgcaggacg gcacgctgat ctacaaggtg
      781 aagatgcgcg gcaccaactt cccccccgac ggccccgtaa tgcagaagaa gaccatgggc
      841 tgggaggcct ccaccgagcg cctgtacccc cgcgacggcg tgctgaaggg cgagatccac
      901 caggccctga agctgaagga cggcggccac tacctggtgg agttcaagac catctacatg
      961 gccaagaagc ccgtgcaact gcccggctac tactacgtgg acaccaagct ggacatcacc
     1021 tcccacaacg aggactacac catcgtggaa cagtacgagc gctccgaggg ccgccaccac
     1081 ctgttcctgg ggcatggcac cggcagcacc ggcagcggca gctccggcac cgcctcctcc
     1141 gaggacaaca acatggccgt catcaaagag ttcatgcgct tcaaggtgcg catggagggc
     1201 tccatgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
     1261 acccagaccg ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc
     1321 ctgtcccccc agttcatgta cggctccaag gcgtacgtga agcaccccgc cgacatcccc
     1381 gattacaaga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
     1441 gacggcggtc tggtgaccgt gacccaggac tcctccctgc aggacggcac gctgatctac
     1501 aaggtgaaga tgcgcggcac caacttcccc cccgacggcc ccgtaatgca gaagaagacc
     1561 atgggctggg aggcctccac cgagcgcctg tacccccgcg acggcgtgct gaagggcgag
     1621 atccaccagg ccctgaagct gaaggacggc ggccgctacc tggtggagtt caagaccatc
     1681 tacatggcca agaagcccgt gcaactgccc ggctactact acgtggacac caagctggac
     1741 atcacctccc acaacgagga ctacaccatc gtggaacagt acgagcgctc cgagggccgc
     1801 caccacctgt tcctgtacgg catggacgag ctgtacaagg gaagcggagc tactaacttc
     1861 agcctgctga agcaggctgg agacgtggag gagaaccctg gacctatgaa aaagcctgaa
     1921 ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg
     1981 atgcagctct cggagggcga agaatctcgt gctttcagct tcgatgtagg agggcgtgga
     2041 tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg
     2101 cactttgcat cggccgcgct cccgattccg gaagtgcttg acattgggga attcagcgag
     2161 agcctgacct attgcatctc ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa
     2221 accgaactgc ccgctgttct gcagccggtc gcggaggcca tggatgcgat cgctgcggcc
     2281 gatcttagcc agacgagcgg gttcggccca ttcggaccgc aaggaatcgg tcaatacact
     2341 acatggcgtg atttcatatg cgcgattgct gatccccatg tgtatcactg gcaaactgtg
     2401 atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat gctttgggcc
     2461 gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa caatgtcctg
     2521 acggacaatg gccgcataac agcggtcatt gactggagcg aggcgatgtt cggggattcc
     2581 caatacgagg tcgccaacat cttcttctgg aggccgtggt tggcttgtat ggagcagcag
     2641 acgcgctact tcgagcggag gcatccggag cttgcaggat cgccgcggct ccgggcgtat
     2701 atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa tttcgatgat
     2761 gcagcttggg cgcagggtcg atgcgacgca atcgtccgat ccggagccgg gactgtcggg
     2821 cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt agaagtactc
     2881 gccgatagtg gaaaccgacg ccccagcact cgtccgaggg caaaggaacg cggctccggc
     2941 gcaacaaatt tctctctcct caaacaagca ggcgatgttg aagaaaatcc cgggcccatg
     3001 tccaatttac tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc aacgagtgat
     3061 gaggttcgca agaacctgat ggacatgttc agggatcgcc aggcgttttc tgagcatacc
     3121 tggaaaatgc ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg
     3181 aaatggtttc ccgcagaacc tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc
     3241 ggtctggcag taaaaactat ccagcaacat ttgggccagc taaacatgct tcatcgtcgg
     3301 tccgggctgc cacgaccaag tgacagcaat gctgtttcac tggttatgcg gcggatccga
     3361 aaagaaaacg ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga acgcactgat
     3421 ttcgaccagg ttcgttcact catggaaaat agcgatcgct gccaggatat acgtaatctg
     3481 gcatttctgg ggattgctta taacaccctg ttacgtatag ccgaaattgc caggatcagg
     3541 gttaaagata tctcacgtac tgacggtggg agaatgttaa tccatattgg cagaacgaaa
     3601 acgctggtta gcaccgcagg tgtagagaag gcacttagcc tgggggtaac taaactggtc
     3661 gagcgatgga tttccgtctc tggtgtagct gatgatccga ataactacct gttttgccgg
     3721 gtcagaaaaa atggtgttgc cgcgccatct gccaccagcc agctatcaac tcgcgccctg
     3781 gaagggattt ttgaagcaac tcatcgattg atttacggcg ctaaggatga ctctggtcag
     3841 agatacctgg cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc
     3901 gctggagttt caataccgga gatcatgcaa gctggtggct ggaccaatgt aaatattgtc
     3961 atgaactata tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct gctggaagat
     4021 ggcgatctcg agccatctgc tggagacatg agagctgcca acctttggcc aagcccgctc
     4081 atgatcaaac gctctaagaa gaacagcctg gccttgtccc tgacggccga ccagatggtc
     4141 agtgccttgt tggatgctga gccccccata ctctattccg agtatgatcc taccagaccc
     4201 ttcagtgaag cttcgatgat gggcttactg accaacctgg cagacaggga gctggttcac
     4261 atgatcaact gggcgaagag ggtgccaggc tttgtggatt tgaccctcca tgatcaggtc
     4321 caccttctag aatgtgcctg gctagagatc ctgatgattg gtctcgtctg gcgctccatg
     4381 gagcacccag tgaagctact gtttgctcct aacttgctct tggacaggaa ccagggaaaa
     4441 tgtgtagagg gcatggtgga gatcttcgac atgctgctgg ctacatcatc tcggttccgc
     4501 atgatgaatc tgcagggaga ggagtttgtg tgcctcaaat ctattatttt gcttaattct
     4561 ggagtgtaca catttctgtc cagcaccctg aagtctctgg aagagaagga ccatatccac
     4621 cgagtcctgg acaagatcac agacactttg atccacctga tggccaaggc aggcctgacc
     4681 ctgcagcagc agcaccagcg gctggcccag ctcctcctca tcctctccca catcaggcac
     4741 atgagtaaca aaggcatgga gcatctgtac agcatgaagt gcaagaacgt ggtgcccctc
     4801 tatgacctgc tgctggaggc ggcggacgcc caccgcctac atgcgcccac tagccgtgga
     4861 ggggcatccg tggaggagac ggaccaaagc cacttggcca ctgcgggctc tacttcatcg
     4921 cattccttgc aaaagtatta catcacgggg gaggcagagg gtttccctgc cacagcttga
     4981 tagcggccgc actcctcagg tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa
     5041 tgccctggct cacaaatacc actgagatct ttttccctct gccaaaaatt atggggacat
     5101 catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat
     5161 agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt
     5221 aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgcccata tgctggctgc
     5281 catgaacaaa ggttggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca
     5341 ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt
     5401 gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt
     5461 cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgga gatccctcga
     5521 cctgcagccc aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     5581 cgctcacaat tccacacaac atacgagccg gatgcataaa gtgtaaagcc tggggtgcct
     5641 aatgagtgaa actcatcaat gtatcttaag gcgtaaattg taagcgttaa tattttgtta
     5701 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
     5761 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
     5821 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
     5881 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
     5941 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
     6001 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
     6061 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
     6121 cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     6181 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     6241 taatattgaa aaaggaagag tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt
     6301 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     6361 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     6421 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     6481 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     6541 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     6601 gaggcctagg cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt
     6661 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     6721 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     6781 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac
     6841 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     6901 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     6961 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     7021 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     7081 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     7141 caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag
     7201 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     7261 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     7321 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     7381 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     7441 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
     7501 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     7561 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     7621 ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga
     7681 cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg
     7741 ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg
     7801 cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg
     7861 cagccaacgt cggggcggca ggccctgcca tagcctcagg ttactcatat atactttaga
     7921 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     7981 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     8041 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     8101 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     8161 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     8221 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     8281 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     8341 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     8401 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     8461 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     8521 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     8581 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     8641 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     8701 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gccatgcatt
     8761 agttattact agcgctaccg gactcagatc tcgagctcaa gcttcgaatt ctgcagtcga
     8821 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
     8881 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     8941 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     9001 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     9061 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     9121 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     9181 gtcatcgcta ttaccatggt cgaggtgagc cccacgttct gcttcactct ccccatctcc
     9241 cccccctccc cacccccaat tttgtattta tttatttttt aattattttg tgcagcgatg
     9301 ggggcggggg gggggggggc gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg
     9361 ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa agtttccttt
     9421 tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc gggcgggagt
     9481 cgctgcgttg ccttcgcccc gtgccccgct ccgcgccgcc tcgcgccgcc cgccccggct
     9541 ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg
     9601 taattagcgc ttggtttaat gacggctcgt ttcttttctg tggctgcgtg aaagccttaa
     9661 agggctccgg gagggccctt tgtgcggggg ggagcggctc ggggggtgcg tgcgtgtgtg
     9721 tgtgcgtggg gagcgccgcg tgcggcccgc gctgcccggc ggctgtgagc gctgcgggcg
     9781 cggcgcgggg ctttgtgcgc tccgcgtgtg cgcgagggga gcgcggccgg gggcggtgcc
     9841 ccgcggtgcg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg tgcgtggggg
     9901 ggtgagcagg gggtgtgggc gcggcggtcg ggctgtaacc cccccctgca cccccctccc
     9961 cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtgcggggcg tggcgcgggg
    10021 ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc ggggccgcct
    10081 cgggccgggg agggctcggg ggaggggcgc ggcggccccg gagcgccggc ggctgtcgag
    10141 gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg cagggacttc
    10201 ctttgtccca aatctggcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg
    10261 gcgcgggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg
    10321 tcgccgcgcc gccgtcccct tctccatctc cagcctcggg gctgccgcag ggggacggct
    10381 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta
    10441 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
    10501 tggttattgt gctgtctcat cattttggca aagaattcct cgaggatgat ccaccggtcg
    10561 cc
//
