LOCUS       sequence-22204-e         685 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Apparsundaram S, Ferguson SM, Blakely RD
  TITLE     Molecular cloning and characterization of a murine 
            hemicholinium-3-sensitive choline transporter.
  JOURNAL   Biochem Soc Trans. 2001 Nov . 29(Pt 6):711-6.
  PUBMED    11709061
REFERENCE   2  (bases 1 to 685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem Soc
            Trans. 2001 Nov . 29(Pt 6):711-6."
COMMENT     Sequence Label: mChT pcDNA3
FEATURES             Location/Qualifiers
     source          1..685
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     51..70
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        51..69
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             147..155
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 gcagagctct ctggctacta gagacccact gcttactggc ttatcgaaat taatacgact
       61 cactataggg agacccaagc ttggtaccga gctcggatcc aaaatgcctt tccatgtgga
      121 aggactggta gctattatcc tcttctacct ccttatattt ctggttggaa tatgggctgc
      181 atggaaaacc aaaaacagcg gcaacccaga agagcgcagt gaagccatca tagtcggggg
      241 ccgtgacatt ggtttgttgg ttggtggttt taccatgaca gccacctggg ttggaggagg
      301 ctacatcaat gggacagcag aagcagtgta tgggccaggt tgtggtctag cttgggctca
      361 ggcacccatt ggatattctc tgagtctaat tttaggtggt ctgttttttg cgaaacctat
      421 gcgttccaag ggatatgtga ctatgttaga cccatttcaa cagatctatg gaaagcgcat
      481 gggtgggctg ctcttcatcc ctgcactgat gggagagatg ttctgggctg cagcaatttt
      541 ctctgcatta ggggccacca tcagcgtgat catttgatgt ggatgtgaac atatcggtca
      601 ttgtctctgc actcattgcc attctttata ccctagtggg tgggctctac tctgtggcat
      661 atactgatgt tgtccagcta ttctg
//
