LOCUS       sequence-222064-       10790 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10790)
  AUTHORS   Peterson TR, Sengupta SS, Harris TE, Carmack AE, Kang SA, Balderas 
            E, Guertin DA, Madden KL, Carpenter AE, Finck BN, Sabatini DM
  TITLE     mTOR Complex 1 Regulates Lipin 1 Localization to Control the SREBP 
            Pathway.
  JOURNAL   Cell. 2011 Aug 5;146(3):408-20.
  PUBMED    21816276
REFERENCE   2  (bases 1 to 10790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2011-08-5"; volume: "146(3)"; pages: "408-20"
COMMENT     Sequence Label: pLKO-puro FLAG SREBP2
FEATURES             Location/Qualifiers
     source          1..10790
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        137..340
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     291..311
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     337..361
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        571..589
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     571..588
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     602..621
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             680..703
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    4175..4292
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4341..4851
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4770..4788
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             4873..5472
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4873..4892)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5369..5389
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5600..5833
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5905..6026
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5942..5961)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5996..6015
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6066..6201
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6221..6240)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6222..6240)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6250..6267)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6250..6266)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6259..6281)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6408..6863
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6495..6514)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6705..6726
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6889..6993
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6994..7062,7063..7854)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7212..7231)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8025..8613
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8514..8533
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8767..8784
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8901..8922
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8937..8942,8943..8960,8961..8967)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8975..8991
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8980..9002
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8999..9015
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8999..9015
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9034..9054
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9036..9054
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9082..9308
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9309..9489
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9536..9661
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10158..10391
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10576..10620
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 accccatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
       61 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      121 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      181 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      241 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
      301 cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga accgtcagat
      361 cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg accgatccag
      421 cctccgcggc cgggaacggt gcattggaac gcggattccc cgtgccaaga gtgacgtaag
      481 taccgcctat agagtctata ggcccacccc cttggcttcg ttagaacgcg gctacaatta
      541 atacataacc ttatgtatca tacacatacg atttaggtga cactatagaa taacatccac
      601 tttgcctttc tctccacagg tgtccactcc caggtccaac tgcacctcgg ttctatcgat
      661 tgaattcacc atggaaatgg actacaagga tgacgatgac aagggtggtg gtggtggtgc
      721 gtcgacgatg gatgagagca gcgagctggg cgttctggag accatggaga ccctcacgga
      781 gctgggcgat gagctgactc tcggggacat cgacgagatg ctacagtttg tcagcaatca
      841 agtgggagag ttccctgatt tgttttcaga gcaactttgt agctccttcc ctggaggtgg
      901 cagcaatggt ggcagtggca acaacagcag tggcagaggc aacaatggtg gagccacaga
      961 ccctgcagta cagcggtcat tcagccaggt cccattatct accttctctc cctcagctgc
     1021 ctcccctcag gctccggccc tgcaggtcaa agtctctcct accccaccaa gggcaacccc
     1081 tgttctccag ccgcgacccc agccccagcc ccaacctcca gctcagctgc agcagcagac
     1141 ggtgatgatc accccgacgt tcagcaccgc tccgcagacg aggatcatcc agcagccttt
     1201 gatataccag aacgcagcga ccagctttca agtcctgcag cctcaagtgc aaagcctcgt
     1261 gacgtccccg caggtgcagc ccgtcaccat ccagcagcag gtgcagacgg tacaggccca
     1321 gcgggtgttg acgcagacag ccaatggcac actgcagacc cttgcccccg ccacggtaca
     1381 gacggtcgct gcgccacagg tgcagcaggt cccggtcctg gtgcaacctc agatcattaa
     1441 gacagattcc cttgtgttga ccacgctgaa gacagatggc agccctgtca tggctgcagt
     1501 ccagaacccg gccctcacgg ccctcactgc ccccatccag acagccgccc ttcaagtgcc
     1561 aaccctggtg ggcagcaacg ggaccattct gaccacaatg cctgtgatga tggggcaaga
     1621 gaaagttcct atcaagcaag tgcctggcgg cgtgaagcag ctggatcctc ccaaagaagg
     1681 agagaggcgg acaacacaca atatcattga aaagcgctac cggtcctcca tcaacgacaa
     1741 aatcatagag ttgaaggact tagtcatggg gacagatgcc aagatgcaca agtctggcgt
     1801 tctgaggaag gccattgatt acatcaaata tctgcagcag gtcaatcaca agctgcgcca
     1861 ggagaacatg gtgctgaagc tggccaatca gaaaaacaag ctcctgaagg gcatcgacct
     1921 gggcagtctg gtggacagtg atgtggactt gaaaattgat gactttaacc agaatgtcct
     1981 tctgatgtct ccgccggcct ccgactccgg gtcccaggcc ggcttctctc cctattccat
     2041 tgactctgag ccgggcagcc ctctgctgga tgacgcaaag gtcaaggatg aaccggactc
     2101 tcctcctgtg gcactgggca tggtggaccg ctctcgaatc ctcttatgtg tcctcacctt
     2161 cctgggcctc tcctttaacc ccttgacttc cttgctgcag tggggagggg cccacaacac
     2221 tgaccagcac ccatactcag gctcgggccg cagtgtactg tcactggagt caggtgctgg
     2281 gggctggttt gactggatgg ttccaactct cctcctgtgg ctggtaaatg gtgtgattgt
     2341 cttgagcgtc tttgtgaagc tgttggtcca cggggagccg gtgattcgcc cacactcacg
     2401 cccctcagtc accttctgga gacaccggaa gcaggcagac ctagacctcg ccaaaggtga
     2461 tttcgcagct gctgctgcca acctacaaac ctgcttatcc gtgctgggcc gggcgctgcc
     2521 cacctcccgc ctggacttgg cctgcagcct ctcctggaat gtgatccgct acagcctgca
     2581 gaagctgcgc ctggtacgct ggttactcaa gaaggtcttc cagcgctggc gggctactcc
     2641 cgccactgca gctggctttg aggatgaggc taagagcagc gcaagggatg cggcactggc
     2701 ctaccacagg ctgcaccagc tgcacatcac agggaagctt ccggcgggat ctgcctgttc
     2761 agatgtgcac atggctctgt gtgctgtgaa cctggccgag tgtgcggagg agaaaatcct
     2821 accaagcaca ctgattgaga tccacctgac agcggccatg gggctcaaga cccgctgtgg
     2881 aggcaagctg ggcttcttgg ctagctactt cttaaatcgt gcccagagtc tgtgtggccc
     2941 agagcatagc actgtccccg actccctgcg ctggctatgc catcccttgg gccagaagtt
     3001 tttcatggag cgaagctggt cgatcaagtc agcagccaag gagagcctgt actgcgccca
     3061 gaggagccca gctgacccca ttgcccaggt acaccaggcg ttctgcaaaa acctgctaga
     3121 aagagcggtg gagtccttgg tgaaacctca ggccaagaag aaggcaggcg accaggaaga
     3181 agagagctgt gaattttcca gtgctctaga gtatttgaaa ttacttcatt cttttgtgga
     3241 ctctgtggga tttgtgacct caccattctc cagcagttcc gtgctcaggt cggcccttgg
     3301 ccccgatgtc atctgccggt ggtggacgtc cgcagtcacc atggccatca gctggctcca
     3361 gggagacgat gcagctgtgc gctctcgttt tactgaagta gagcgggtcc ccaaggccct
     3421 ggaagtgacc gagagtccct tggtgaaggc cgtcttctat acctgtagag ccatgcatgc
     3481 ctccctctct gggaaagctg acgggcagca gaactccttc tgtcactgtg agagggccag
     3541 tggtcacctg tggagcagtc tcaacgtcag cggcactact tctgatcctt cacttaacca
     3601 tgtgatccag ctgttcacct gtgacctgct actgtcgctg aggacagcac tctggcaaaa
     3661 gcaggccagt gccagccagc tcctgggtga gacctaccat gcatccggca ctgagctggc
     3721 cggcttccag cgggacctgg gcagccttcg aaggctggcc catagcttcc gcccagccta
     3781 ccgcaaggtg ttcctgcatg aagccactgt gcgcctgatg gcaggagcca gccctacccg
     3841 tacacaccag ctgctggagc atagcctacg gcggcgcccc actcagaaca ccaagcatgg
     3901 agaggtagac acctggcctg gccaacgaga gcgagctact gccatcctgc tggcctgccg
     3961 ccatcttccc ctctctttcc tgtcttcccc gggccagcgg gcagtgctgc tggctgaagc
     4021 tgcccgcacc ctggagaagg tgggtgaccg ccgttcctgc agtgactgcc agcagatgat
     4081 tgtcaagctg ggcggtggca ctgccatcgc tgcctcctga gcggccgcag tgagaattct
     4141 cgacctcgag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
     4201 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     4261 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
     4321 cactttggcc gcggctcgag ggggttgggg ttgcgccttt tccaaggcag ccctgggttt
     4381 gcgcagggac gcggctgctc tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg
     4441 tctcgcacat tcttcacgtc cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg
     4501 ggccccccgg cgacgcttcc tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg
     4561 gcgtgccgga cgtgacaaac ggaagccgca cgtctcacta gtaccctcgc agacggacag
     4621 cgccagggag caatggcagc gcgccgaccg cgatgggctg tggccaatag cggctgctca
     4681 gcagggcgcg ccgagagcag cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg
     4741 gtagtgtggg ccctgttcct gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc
     4801 acgtcggcag tcggctccct cgttgaccga atcaccgacc tctctcccca gggggatcca
     4861 ccggagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     4921 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     4981 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     5041 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     5101 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     5161 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     5221 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     5281 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     5341 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     5401 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     5461 cccggtgcct gacgcccgcc ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc
     5521 catgcatcgg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     5581 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
     5641 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     5701 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     5761 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     5821 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     5881 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
     5941 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     6001 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc
     6061 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     6121 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     6181 gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta
     6241 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     6301 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     6361 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
     6421 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     6481 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     6541 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     6601 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     6661 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     6721 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     6781 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     6841 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct
     6901 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     6961 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     7021 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     7081 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     7141 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     7201 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     7261 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     7321 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     7381 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     7441 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     7501 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     7561 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     7621 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     7681 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     7741 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     7801 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     7861 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     7921 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     7981 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     8041 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     8101 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     8161 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     8221 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     8281 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     8341 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     8401 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     8461 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     8521 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     8581 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     8641 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     8701 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     8761 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     8821 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     8881 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     8941 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     9001 ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa
     9061 aagctggagc tgcaagctta atgtagtctt atgcaatact cttgtagtct tgcaacatgg
     9121 taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg
     9181 tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga
     9241 ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat
     9301 acataaacgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     9361 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     9421 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     9481 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     9541 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     9601 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     9661 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     9721 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     9781 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     9841 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     9901 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     9961 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccggccgct gatcttcaga
    10021 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
    10081 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
    10141 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
    10201 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
    10261 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
    10321 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
    10381 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
    10441 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
    10501 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
    10561 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
    10621 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
    10681 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
    10741 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
//
