LOCUS       sequence-222066-        7809 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Knock-in of P2A-eGFP at the mouse Sox1 gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7809)
  AUTHORS   Deluz C, Friman ET, Strebinger D, Benke A, Raccaud M, Callegari A, 
            Leleu M, Manley S, Suter DM
  TITLE     A role for mitotic bookmarking of SOX2 in pluripotency and 
            differentiation.
  JOURNAL   Genes Dev. 2016 Nov 15;30(22):2538-2550. doi: 
            10.1101/gad.289256.116. Epub 2016 Dec 5.
  PUBMED    27920086
REFERENCE   2  (bases 1 to 7809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.289256.116"; journalName: "Genes Dev"; date: 
            "2016-11-15- 15"; volume: "30"; issue: "22"; pages: "2538-2550"
COMMENT     Sequence Label: pKISox1-P2A-eGFP-Hygro
FEATURES             Location/Qualifiers
     source          1..7809
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1136..1192
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(1199..1201,1202..1915)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(1199..1201,1202..1912)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1241..1262)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1502..1521)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1849..1870
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(1922..1955)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        1977..2487
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2406..2424
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2502..3527
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             2502..3527
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    3669..3750
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3706..3725)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3760..3779
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(3795..3828)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      complement(4872..5327)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5009..5030)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5221..5240
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5354..5458
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5460..5817
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5460..5651
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5483..5503)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5668..5803
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5852..6646
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5852..6646
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5906..5925)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6516..6535
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6834..6853)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6878..6925
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      join(7254..7809,1..33)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7743..7762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
       61 ttcctggcct tttgctggcc ttttgctcac atgtgaattc gagacggtcg acgctagctt
      121 catggtgtgg tcccgcggac agcggcgcaa gatggcccag gaaaacccca agatgcacaa
      181 ctcggagatc agcaagcgcc tcggggccga gtggaaggtc atgtccgagg ccgagaagcg
      241 gccgttcatc gacgaggcca agagactgcg cgcgctgcac atgaaggaac acccggatta
      301 caagtaccgg ccgcgccgca agaccaagac gctgctcaag aaggacaagt actcgctggc
      361 cggcgggctg ctagcggccg gcgcgggtgg cggcggcgcg gccgtggcca tgggtgtggg
      421 cgtgggcgtc ggggcggcgg cggtgggcca gcgcttggag agcccaggcg gcgcggcggg
      481 aggaggctac gcgcatgtca acggctgggc taacggcgcc taccccggct cggtggccgc
      541 ggcggctgcg gccgcggcca tgatgcagga ggcacagctg gcctacgggc agcacccagg
      601 cgcgggcggc gcgcacccgc acgcacaccc ggcgcacccg cacccgcacc atccgcacgc
      661 gcatcctcac aacccgcagc ccatgcaccg ctacgacatg ggcgcgctgc agtacagccc
      721 catctccaac tctcagggct acatgagcgc gtcgccttcg ggctacggcg gcatccctta
      781 cggcgccgcg gccgccgccg ccgccgctgc gggcggcgcg caccagaact cggcggtggc
      841 ggcagcggca gccgcggcag ccgcgtcgtc gggggccctg ggcgccctcg gatctctggt
      901 caagtcggag ccgagcggca gtccgccggc cccggctcac tcacgggcac cgtgtcccgg
      961 ggacctgcgc gagatgatca gcatgtacct gcccgccggc gagggtggcg acccggcggc
     1021 ggcagcggct gcggcggccc aaagccggct gcactcgctg ccacagcact accagggcgc
     1081 gggcgcgggc gtcaacggca cggtgcccct gacgcacatc gatatcggaa gcggagctac
     1141 taacttcagc ctgctgaagc aggctggaga cgtggaggag aaccctggac ctaacgttgt
     1201 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     1261 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     1321 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     1381 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
     1441 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     1501 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     1561 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     1621 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
     1681 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     1741 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     1801 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     1861 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaagagct
     1921 cataacttcg tatagcatac attatacgaa gttatatcga tatatcgaat tcccacgggg
     1981 ttggggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg
     2041 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt
     2101 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct
     2161 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa
     2221 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc
     2281 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc
     2341 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc
     2401 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt
     2461 gaccgaatca ccgacctctc tccccagggg gatccgccac catgaaaaag cctgaactca
     2521 ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc
     2581 agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg
     2641 tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact
     2701 ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc
     2761 tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg
     2821 aactgcccgc tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc
     2881 ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat
     2941 ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg
     3001 acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg
     3061 actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg
     3121 acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat
     3181 acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc
     3241 gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc
     3301 tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag
     3361 cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta
     3421 cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg
     3481 atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa ggaatagctc gagttccggt
     3541 gaaacaggcg gccgcgactc tagatcataa tcagccatac cacatttgta gaggttttac
     3601 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     3661 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     3721 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     3781 atgtatcttt taaaataact tcgtatagca tacattatac gaagttatta agctctagag
     3841 acactgcggc ttaaggccgg cgccccggcg acgaagagcg aggcctgcgc cccagcctcc
     3901 agagcccgac tttgtaccga ggtccccgcg ctctcgataa aaggccgctc tggagagccg
     3961 agcgccaggt gacatctgcc cccatcacct tccccaggac tccgaggcgc tgacaccaga
     4021 ctggcctctt agactgaact ttggtgtttt catgagacct tttgtacagt atttatcgtc
     4081 cgcagaggag gcacacagcg ttttctcggc ttcggaggac aaaagacaaa aacccagcga
     4141 ggcgatgcca acttttgtat gactgccggc tctgtaactt tttccggggt ttacttcccg
     4201 ccagctcttc tgcctgaggc cgagtgacgg acctcgagcc cttctcactt gttataaatc
     4261 taagtaaggc agatccaaac atttacaagt tttttgtagt tgttaccgct cttttgggtt
     4321 ggttggttaa tttataccgc aatcccctct cagacggtgg agttatattc tgggttttgt
     4381 aaatctctgt atccgagcat ttccaatttt ttgttttgtt ttgtattatt tcttgtaaat
     4441 gcgttgtgac atttttattt taggcgttgc gatacggggg gaagaggagt cggatgttgt
     4501 acatagcctg caagtctttc atctaaaagc aaaaaacaaa gagagatacc cccaaaatgc
     4561 atcaaatttg aatcaataca tttaagagag aaaagcccgt ttccctgtga aatcgaaacg
     4621 tgctaatttt atatgcatgt tttatgagtt caaaatacaa ttagcaatct gttggaggag
     4681 aaattatctg cagggcccaa gagtaaggaa accccaccaa gcggagtgca gcgtttccgt
     4741 gactttattc ggtggggaaa gagaaatctt aaactccata gattattttg tagagttcaa
     4801 gttgacacag aagggaagta attacaaaat gcttcctcta gactcaggta ccgcatatgc
     4861 ttaaaaggcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     4921 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     4981 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     5041 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     5101 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     5161 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     5221 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     5281 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt
     5341 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     5401 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc
     5461 tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     5521 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     5581 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     5641 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     5701 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     5761 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat
     5821 cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
     5881 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     5941 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     6001 gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct
     6061 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
     6121 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
     6181 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
     6241 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
     6301 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
     6361 gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
     6421 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     6481 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     6541 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
     6601 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
     6661 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc
     6721 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     6781 cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca
     6841 cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa
     6901 acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct
     6961 gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca
     7021 ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc
     7081 cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa
     7141 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     7201 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     7261 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     7321 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     7381 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     7441 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     7501 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     7561 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     7621 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     7681 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     7741 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     7801 cgatttttg
//
