LOCUS       sequence-222104-        5200 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multisite gateway vector for 3' tagging with dominant negative 
            Dynamin2b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5200)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 5200)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: p3E-Dnm2b-DN
FEATURES             Location/Qualifiers
     source          1..5200
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..125)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2686..2781)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2818..2837)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2819..2837)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2842..2858)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2842..2858)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2960..2979)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2971..3780
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3038..3057)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3927..4515
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4416..4435
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4669..4686
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4845..4872)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4964..5050)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     5099..5121
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5113..5130
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5114..5130
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 catagtgact gcatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc
       61 taatttaata tattgatatt tatatcattt tacgtttctc gttcaacttt cttgtacaaa
      121 gtggtgggga accgcgggat ggaggacttg attcccctca ttaacaagct gcatgacgcc
      181 ttcagctcca tcggccagag ctgcaacctg gagctgccgc agatcgcggt ggtcggcgtg
      241 cagagtgccg gcgcgacctc cgtgctcgag aacttcgtgg gaagggactt tctgccgaga
      301 ggaccaggaa tcgtcactcg caggcctctg attctccagc tggtgaacag taaagctgaa
      361 tatgccgagt tcctgcactg taagggccgc aagtttgtgg actttgacga ggttcggatg
      421 gagattgaag cagaaacaga ccgaatcact gaatctaaca ttggaatctc tgcagtgccc
      481 attaacctgc gcctttactc gcccaacgtg ctgaatctga cgctgatcga tcttccgggc
      541 ataacgaagg tggcggtggg tgaccagccg caggatatcg agtaccagat ccgggatatg
      601 gtgttgcagt tcatctccaa agagaactgt ctgattctgg ccatcactcc agcaaacatg
      661 gacctgacca actctgacgc tctcaagatc agcaaagagg tggaccctca tggtctgcgc
      721 accatcggtg tgatcaccaa actggacctg atggatgaag gaaccgacgc cagagacatc
      781 ctggagaaca agctgctgcc gctgcgcaga ggttatatcg gtgtggtgaa ccgcagtcag
      841 aaggacattg atggcaggaa ggacatccgc gcggctcttt ctgctgagag gaagttcttc
      901 ctgtcccatc cagcatacag acacatagca gagagaatgg gcacaccgta cctgcagaaa
      961 acactcaacc agcaactgat gaaccacatc cgcgacacac tgccggcgct gcgcagtaaa
     1021 ctgcagtctc agctgctgtc gctggagaaa gaggtggagg agtacaagaa cttcaaacca
     1081 gacgatcctg cgcgcaaaac caaagccctg ctgcagatgg tccagcagtt cggtgtggac
     1141 tttgagaagc gtataggggg ctctggagat caggtggaca ctctggaact ctccggcgga
     1201 gctcgaataa accgcatctt ccacgagcgc ttccccttcg agctggtgaa gacggagttt
     1261 gatgagaagg agttgcggag ggagatcagt tacgccatca agaacattca cggagtcagg
     1321 acggggctct tcaccccaga tctggccttc gaggccatcg tgaaaaagca gctgataaag
     1381 ctgaaggagc cgtgcctcaa gtgcatcgac ctggtcatcc aggagctgat caacacggtc
     1441 aggcagagca cactgaagct gaagttgtat cctcggctga gagaagagac ggagagaatc
     1501 atcacaatat atgtcagaga gagagagacc aaaaccaaag accagatgat gctgctgatc
     1561 gatgtccagc tctcctacat caacacaaac cacgaggact tcatcggatt cgccaatgcg
     1621 caacagagca gcagagatgt cactaagaag caagccgttc ccaatcaggt gatccgccgt
     1681 ggttggctga ccatcaacat cagcatcatg aagggcggct ccaaagacta ctggttcgtg
     1741 ctgacggcgg agtctctgtc ctggtacaag gatgaggagg agaaggagaa gaagttcatg
     1801 ctgccgctgg ataacctgaa gctgcgggat gtggagaaga gcttcatgag caacaaacac
     1861 accttcggca tcttcaacac cgagcagagg aacgtgtata aagacctgcg tcagatcgag
     1921 ctggcctgcg ccactcagga ggatgtggac agctggaagg cctctttcct cagagcggga
     1981 gtctatcctg agaaagacca ggtggagaac ggggacgtgt cagcagactt tctctccatg
     2041 gaccctcagc tggagcgtca ggttgagacc atcagtaatc tggtggactc gtacatcagc
     2101 atcgtcaaca agagcatccg agacctgatg cccaaaacca tcatgcacct gatgatcaac
     2161 agcacgaagg agttcatcta ctcggagctg ctggcgtacc tgtactcgtc aggtgatcag
     2221 aacagtctga tggaggagtc ggcggagcag gagcagcggc gggacgagat gctccgcatg
     2281 tacaacgctc tgaaagaggc tctgaagatc atcggggacg tcagcacatc caccttcacc
     2341 acacctgtgc ccgcgccggt ggacgacacc tggatacaca ctgaccccag tcctccgtct
     2401 cagcgtaagc cagctccgcc cactgtccgc cctcctgcag tgcgaggctc cacccccggt
     2461 gctccagccc caccttctct aaacccctcc cctgccttcg gagctccgcc tgttccctcc
     2521 agacccacca cattggagcc agctgtagct ccgccccctt gtgttccatc tcgccctgca
     2581 caggtcccgc ccactgtgct gcccgggata cgcagtagga ggcctccagc ggctccaaac
     2641 aggcccacaa tcatccgtcc gacagaacca tctctgctgg actgacaact ttattataca
     2701 aagttggcat tataaaaaag cattgcttat caatttgttg caacgaacag gtcactatca
     2761 gtcaaaataa aatcattatt tggagctcca tggtagcgtt aacgcggccg cgatatcccc
     2821 tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa
     2881 aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct
     2941 gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg
     3001 ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat
     3061 gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg
     3121 tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta
     3181 aactggctga cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat
     3241 gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt attagaagaa
     3301 tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat
     3361 tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg
     3421 caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc
     3481 tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca
     3541 gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata
     3601 ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta
     3661 tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt
     3721 attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa
     3781 tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc
     3841 gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac
     3901 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     3961 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     4021 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     4081 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     4141 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     4201 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     4261 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     4321 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     4381 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     4441 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     4501 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     4561 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     4621 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     4681 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     4741 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     4801 attaatacgc gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca
     4861 ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc
     4921 ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga
     4981 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc
     5041 gttttatttg atgcctggca gttccctact ctcgcgttaa cgctagcatg gatgttttcc
     5101 cagtcacgac gttgtaaaac gacggccagt cttaagctcg ggccctgcag ctctagagct
     5161 cgaattctac aggtcactaa taccatctaa gtagttggtt
//
