LOCUS       sequence-222109-        4125 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector encoding WT ScCas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Chatterjee P, Jakimo N, Jacobson JM
  TITLE     Minimal PAM specificity of a highly similar SpCas9 ortholog.
  JOURNAL   Sci Adv. 2018 Oct 24;4(10):eaau0766. doi: 10.1126/sciadv.aau0766. 
            eCollection 2018 Oct.
  PUBMED    30397647
REFERENCE   2  (bases 1 to 4125)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-10-24"; volume: "4(10):eaau0766. doi"; pages: " 
            10.1126/sciadv.aau0766. eCollection 2018 Oct"
COMMENT     Sequence Label: pScCas9
FEATURES             Location/Qualifiers
     source          1..4125
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..4125
                     /codon_start=1
                     /product="Cas9 endonuclease from the Streptococcus canis 
                     Type II CRISPR/Cas system (Chatterjee et al., 2018)"
                     /label=ScCas9
                     /note="recognizes minimal 5'-NNG-3' PAM sequences"
                     /translation="MEKKYSIGLDIGTNSVGWAVITDDYKVPSKKFKVLGNTNRKSIKK
                     NLMGALLFDSGETAEATRLKRTARRRYTRRKNRIRYLQEIFANEMAKLDDSFFQRLEES
                     FLVEEDKKNERHPIFGNLADEVAYHRNYPTIYHLRKKLADSPEKADLRLIYLALAHIIK
                     FRGHFLIEGKLNAENSDVAKLFYQLIQTYNQLFEESPLDEIEVDAKGILSARLSKSKRL
                     EKLIAVFPNEKKNGLFGNIIALALGLTPNFKSNFDLTEDAKLQLSKDTYDDDLDELLGQ
                     IGDQYADLFSAAKNLSDAILLSDILRSNSEVTKAPLSASMVKRYDEHHQDLALLKTLVR
                     QQFPEKYAEIFKDDTKNGYAGYVGIGIKHRKRTTKLATQEEFYKFIKPILEKMDGAEEL
                     LAKLNRDDLLRKQRTFDNGSIPHQIHLKELHAILRRQEEFYPFLKENREKIEKILTFRI
                     PYYVGPLARGNSRFAWLTRKSEEAITPWNFEEVVDKGASAQSFIERMTNFDEQLPNKKV
                     LPKHSLLYEYFTVYNELTKVKYVTERMRKPEFLSGEQKKAIVDLLFKTNRKVTVKQLKE
                     DYFKKIECFDSVEIIGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTL
                     FEDREMIEERLKTYAHLFDDKVMKQLKRRHYTGWGRLSRKMINGIRDKQSGKTILDFLK
                     SDGFSNRNFMQLIHDDSLTFKEEIEKAQVSGQGDSLHEQIADLAGSPAIKKGILQTVKI
                     VDELVKVMGHKPENIVIEMARENQTTTKGLQQSRERKKRIEEGIKELESQILKENPVEN
                     TQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFIKDDSIDNKVLTRSVE
                     NRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSEADKAGFIKRQL
                     VETRQITKHVARILDSRMNTKRDKNDKPIREVKVITLKSKLVSDFRKDFQLYKVRDINN
                     YHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKRFFY
                     SNIMNFFKTEVKLANGEIRKRPLIETNGETGEVVWNKEKDFATVRKVLAMPQVNIVKKT
                     EVQTGGFSKESILSKRESAKLIPRKKGWDTRKYGGFGSPTVAYSILVVAKVEKGKAKKL
                     KSVKVLVGITIMEKGSYEKDPIGFLEAKGYKDIKKELIFKLPKYSLFELENGRRRMLAS
                     ATELQKANELVLPQHLVRLLYYTQNISATTGSNNLGYIEQHREEFKEIFEKIIDFSEKY
                     ILKNKVNSNLKSSFDEQFAVSDSILLSNSFVSLLKYTSFGASGGFTFLDLDVKQGRLRY
                     QTVTEVLDATLIYQSITGLYETRTDLSQLGGD"
ORIGIN
        1 atggaaaaaa aatactcaat cggattggat atcggcacta actcagttgg atgggctgta
       61 attacagacg attacaaggt ccctagcaaa aagtttaagg tgctgggtaa tacgaatcgc
      121 aagagtatta agaagaatct gatgggtgca ttactgtttg attctggcga aacagccgaa
      181 gctactcgtt taaagcgcac cgcccgccgt cgttatacac gccgcaagaa tcgcattcgc
      241 tatcttcaag aaatcttcgc taatgaaatg gcgaagctgg acgactcttt cttccagcgt
      301 ttggaggaaa gttttcttgt ggaggaggac aaaaaaaatg aacgtcaccc cattttcggg
      361 aacttagcgg acgaggtcgc ctatcaccgt aactatccca ctatttacca cctgcgcaaa
      421 aagcttgcgg attctccaga aaaggctgac cttcgcctga tttatctggc attggcacat
      481 atcatcaaat ttcgtgggca cttccttatt gaaggaaaat tgaacgcaga aaattctgat
      541 gtcgcaaaac tgttctacca gttgatccag acatataatc aattattcga ggagagtccc
      601 ctggatgaaa tcgaagtgga tgcaaagggt atcctgtccg cccgtctgtc aaagagtaaa
      661 cgtcttgaaa agctgattgc ggtgtttccc aatgaaaaaa agaacggttt gttcgggaac
      721 attatcgctc tggcccttgg tttaacccct aactttaagt caaactttga cctgactgag
      781 gacgcgaaat tacagctttc taaagatact tatgacgatg acttagatga gcttctgggg
      841 caaattgggg accagtacgc cgatcttttc agtgcagcca agaatcttag tgacgcgatt
      901 ttgctttcag acatcttacg ttccaacagc gaagtaacaa aggcgccact gagcgcctct
      961 atggttaagc gttatgacga gcaccatcag gaccttgcac ttcttaagac tctggtgcgt
     1021 caacagtttc cagaaaagta cgcagagatc ttcaaggacg atacaaaaaa cggatacgcc
     1081 gggtatgtcg gaattggaat taaacatcgc aagcgcacaa ctaaactggc gacacaagaa
     1141 gagttctaca aattcattaa acctattctt gagaaaatgg acggggccga ggagctgttg
     1201 gccaaactga accgtgacga ccttttacgt aaacagcgca cctttgacaa cggttccatt
     1261 cctcaccaga tccatcttaa agaattgcac gcaattttgc gtcgtcagga ggagttttat
     1321 ccatttctga aagagaatcg tgagaaaatt gagaaaatcc ttacgttccg catcccctac
     1381 tatgttggtc ctctggctcg cggcaactcc cgcttcgcgt ggttgacacg taagtctgag
     1441 gaggctatca ctccctggaa cttcgaggaa gtggtggaca aaggagccag cgcacagagc
     1501 tttatcgaac gtatgacaaa tttcgacgaa cagcttccaa acaaaaaagt cttaccgaag
     1561 catagtcttt tatatgagta tttcaccgtc tataatgagt taacgaaagt gaagtacgtt
     1621 actgagcgca tgcgtaagcc cgagttttta agtggcgagc aaaaaaaggc tattgtcgat
     1681 cttctgttta agactaaccg caaagtcacc gtgaaacaac ttaaagaaga ttattttaag
     1741 aagatcgaat gtttcgattc ggtagaaatt attggcgtag aggatcgttt caacgctagt
     1801 ttggggacat accatgactt acttaagatc atcaaggaca aggatttttt ggataatgaa
     1861 gaaaacgagg atattctgga ggacattgta ttgaccctga cactgttcga ggaccgtgaa
     1921 atgattgaag agcgtttaaa aacatatgcg cacctgtttg acgataaagt gatgaagcaa
     1981 ttaaagcgcc gtcattacac tgggtggggt cgtctttctc gtaaaatgat taacggtatc
     2041 cgtgataaac agtctggtaa gacgattctg gacttcctta agagcgatgg cttctcgaac
     2101 cgtaacttta tgcaactgat tcacgatgac tccctgacgt ttaaagaaga aattgagaag
     2161 gctcaagtct ctgggcaagg ggacagtctg cacgagcaaa ttgctgacct tgccggaagt
     2221 cccgcgatta agaaaggaat cttgcaaaca gtaaaaattg tggatgaatt ggttaaagtg
     2281 atgggacata aacccgaaaa tattgtcatc gagatggcgc gcgagaacca gaccactacc
     2341 aaggggcttc aacaatcgcg tgaacgtaag aaacgcatcg aagaaggcat taaggagttg
     2401 gagagccaaa ttctgaaaga aaatccagta gagaatactc agttacagaa tgaaaaattg
     2461 tatctttatt acttacagaa cggacgcgat atgtacgtgg atcaagagct ggacatcaat
     2521 cgcctgagtg actacgacgt agatcatatc gttcctcagt cgtttatcaa ggatgacagc
     2581 atcgacaata aagtgctgac gcgtagtgtc gaaaatcgcg gaaaatcgga taatgtgcca
     2641 tcggaggaag tcgtgaaaaa aatgaagaat tattggcgcc aacttttgaa cgccaagttg
     2701 attacccaac gtaaattcga taatcttacc aaggctgaac gcgggggcct gtccgaagca
     2761 gacaaagcag ggtttattaa gcgtcaactt gtagagacac gtcagattac aaagcatgtc
     2821 gcccgcattc ttgattcgcg catgaatact aagcgtgata aaaatgacaa gcccattcgt
     2881 gaggttaaag taattacact gaagtctaag ctggttagtg actttcgtaa agacttccag
     2941 ttatacaagg tgcgcgatat caataattat catcacgccc atgacgctta tctgaatgcg
     3001 gttgtaggta cggccctgat caagaagtat ccaaaattag agtccgagtt cgtgtacggt
     3061 gattacaagg tctacgatgt ccgtaaaatg attgccaaat ccgaacaaga aatcggtaaa
     3121 gcgactgcaa aacgcttctt ctattctaac attatgaatt tctttaagac cgaggtaaaa
     3181 ttggcgaacg gggaaattcg taaacgtccg ttgatcgaga caaacggaga aacgggcgag
     3241 gttgtttgga acaaagaaaa agacttcgcc actgttcgta aagttctggc gatgccgcag
     3301 gtaaacatcg taaagaaaac cgaagttcaa actggaggat tttctaaaga atcaatcctt
     3361 tctaaacgtg aatcggcgaa gttgattccg cgcaaaaaag ggtgggacac tcgtaaatac
     3421 ggcggttttg ggagtccgac tgtagcctat agtatcctgg tggttgcaaa ggtcgagaag
     3481 ggaaaagcta aaaaactgaa atccgttaag gtcttggttg ggatcacgat tatggagaaa
     3541 ggaagctatg aaaaggaccc tattggtttt ctggaagcca aaggctacaa ggatatcaaa
     3601 aaggagttga tcttcaaatt acctaaatat tcattgttcg aactggagaa cggacgccgt
     3661 cgtatgttag cttctgcaac cgagttgcag aaggctaacg aactggtatt gccacaacat
     3721 ctggtgcgtt tactttatta tacacagaac atctcggcta cgacaggctc taacaatttg
     3781 gggtacatcg agcaacatcg tgaagaattc aaggagattt tcgaaaaaat tattgatttc
     3841 tcggagaagt atatcttgaa aaataaagtg aattcaaact tgaaatcgag ttttgacgag
     3901 caattcgcgg tatcggattc cattctgttg tccaacagct ttgttagcct gttgaaatat
     3961 acctcttttg gcgcgtcggg aggattcacc ttcctggacc ttgacgttaa gcaagggcgc
     4021 ttgcgctacc agacggttac ggaggttctg gatgcgacct tgatctatca aagcatcacc
     4081 ggactgtacg agacacgtac agatctgagc caactgggag gagac
//
