LOCUS       sequence-222124-         706 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mutation on stability/solubility of SOD1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 706)
  TITLE     Fisher Lab SOD1 plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pmSod1-D83G-N'His
FEATURES             Location/Qualifiers
     source          1..706
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    20..44
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             59..81
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             100..117
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             127..144
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             619..636
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
       61 tgtttaactt taagaaggag atataccatg ggcagcagcc atcatcatca tcatcacagc
      121 agcggcctgg tgccgcgcgg cagccatatg gcgatgaaag cggtgtgcgt gctgaagggc
      181 gacggtccgg tgcagggaac catccacttc gagcagaagg caagcggtga accagttgtg
      241 ttgtcaggac aaattacagg attaactgaa ggccagcatg ggttccacgt ccatcagtat
      301 ggggacaata cacaaggctg taccagtgca ggacctcatt ttaatcctca ctctaagaaa
      361 catggtggcc cggcggatga agagaggcat gttggagacc tgggcaatgt gactgctgga
      421 aaggacggtg tggccaatgt gtccattgaa gatcgtgtga tctcactctc aggagagcat
      481 tccatcattg gccgtacaat ggtggtccat gagaaacaag atgacttggg caaaggtgga
      541 aatgaagaaa gtacaaagac tggaaatgct gggagccgct tggcctgtgg agtgattggg
      601 attgcgcagt aactcgagca ccaccaccac caccactgag atccggctgc taacaaagcc
      661 cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactag
//
