LOCUS       sequence-222128-         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mutation on stability/solubility of SOD1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  TITLE     Fisher Lab SOD1 plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: phSOD1-E100G-N'His
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    20..44
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             59..81
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             100..117
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             127..144
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             724..741
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
       61 tgtttaactt taagaaggag atataccatg ggcagcagcc atcatcatca tcatcacagc
      121 agcggcctgg tgccgcgcgg cagccatatg gcgacgaagg ccgtgtgcgt gctgaagggc
      181 gacggcccag tgcagggcat catcaatttc gagcagaagg aaagtaatgg accagtgaag
      241 gtgtggggaa gcattaaagg actgactgaa ggcctgcatg gattccatgt tcatgagttt
      301 ggagataata cagcaggctg taccagtgca ggtcctcact ttaatcctct atccagaaaa
      361 cacggtgggc caaaggatga agagaggcat gttggagact tgggcaatgt gactgctgac
      421 aaagatggtg tggccgatgt gtctattgga gattctgtga tctcactctc aggagaccat
      481 tgcatcattg gccgcacact ggtggtccat gaaaaagcag atgacttggg caaaggtgga
      541 aatgaagaaa gtacaaagac aggaaacgct ggaagtcgtt tggcttgtgg tgtaattggg
      601 atcgcccaat aaacattccc ttggatgtag tctgaggccc cttaactcat ctgttatcct
      661 gctagctgta gaaatgtatc ctgataaaca ttaaacactg taatcttaaa atgataactc
      721 gagcaccacc accaccacca ctgagatccg gctgctaaca aagcccgaaa ggaagctgag
      781 ttggctgctg ccaccgctga gcaataacta g
//
