LOCUS       sequence-222131-       15576 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15576)
  AUTHORS   Cai D, Cohen KB, Luo T, Lichtman JW, Sanes JR
  TITLE     Improved tools for the Brainbow toolbox.
  JOURNAL   Nat Methods. 2013 May 5;10(6):540-7. doi: 10.1038/nmeth.2450. Epub 
            2013 May 5.
  PUBMED    23817127
REFERENCE   2  (bases 1 to 15576)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2450"; journalName: "Nat Methods"; date: "2013-05-5- 
            5"; volume: "10"; issue: "6"; pages: "540-7"
COMMENT     Sequence Label: pThy1-Brainbow3.2
FEATURES             Location/Qualifiers
     source          1..15576
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6239..6263
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6312..6345)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(6354..6387)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(6396..6429)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     regulatory      6438..6447
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6450..6470
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6471..7175
                     /codon_start=1
                     /product="Phialidium yellow fluorescent proteim"
                     /label=PhiYFP
                     /note="mammalian codon-optimized"
                     /translation="MSSGALLFHGKIPYVVEMEGNVDGHTFSIRGKGYGDASVGKVDAQ
                     FICTTGDVPVPWSTLVTTLTAGAQCFAKYGPELKDFYKSCMPDGYVQERTITFEGDGNF
                     KTRAEVTFENGSVYNRVKLNGQGFKKDGHVLGKNLEFNFTPHCLYIWGDQANHGLKSAF
                     KICHEITGSKGDFIVADHTQMNTPIGGGPVHVPEYHHMSYHVKLSKDVTDHRDNMSLKE
                     TVRAVDCRKTYL"
     polyA_signal    7358..7479
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(7492..7525)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      7534..7543
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7540..8247
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     misc_feature    8335..8923
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(8806..8817)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    9057..9178
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(9195..9228)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      9237..9246
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(9243..9245,9246..9248,9249..9959)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(9243..9245,9246..9248,9249..9959)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9288..9309)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9549..9568)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9896..9917
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    10047..10635
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(10518..10529)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    10769..10890
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(10909..10942)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     regulatory      10951..10960
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10957..11652
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     misc_feature    11740..12328
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(12211..12222)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(12576..12600)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(15407..15426)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     15526..15548
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcaggatc gcggccgcga tccccgggcg agctcgaatt agttacgcta
     2101 gggataacag ggtaatatag aaaattcaga gaccgggaac caaactagcc tttaaaaaac
     2161 ataagtacag gagccagcaa gatggctcag tgggtaaagg tgcctaccag caagcctgac
     2221 agcctgagtt cagtccccac gaactacgtg gtaggagagg accaaccaac tctggaaatc
     2281 tgttctgcaa acacatgctc acacacacac acacaaatag tataaacaat tttaaatttc
     2341 atttaaaaat aatttgtaaa caaaatcatt agcacaggtt ttagaaagag cctcttggtg
     2401 acatcaagtt gatgctgtag atggggtatc attcctgagg acccaaaacc gggtctcagc
     2461 ctttccccat tctgagagtt ctctcttttc tcagccacta gctgaagagt agagtggctc
     2521 agcactgggc tcttgagttc ccaagtccta caactggtca gcctgactac taaccagcca
     2581 tgaagaaaca aggagtggat gggctgagtc tgctgggatg ggagtggagt tagtaagtgg
     2641 ccatggatgt aatgacccca gcaatgctgg ctagaaggca tgcctccttt ccttgtctgg
     2701 agacggaacg ggagggatca tcttgtactc acagaaggga gaacattcta gctggttggg
     2761 ccaaaatgtg caagttcacc tggaggtggt ggtgcatgct tttaactcca gtactcagga
     2821 ggcagggcca ggtggatctc tgtgagttca agaccagcct gcactatgga gagagttttg
     2881 ggacagccag agttacacag aaaaatcctg gtggaaaatc tgaaagaaag agagaaagaa
     2941 agaaagaaag aaaggaagaa agaaagaaag agtggcaggc aggcaggcag gaggaaggaa
     3001 ggaaggaagg aaggaaggaa ggaaggaagg aaggaaaata ggtgcgactt caagatccgg
     3061 agttacaagc agaatgcact gtttccctaa cagggccaag tgttttgagt aactgaaggt
     3121 gggcatgatg cctgggaagc agaaacaagc caggcagatg caccccttgc cttgcttccg
     3181 aagggctgca gtagcatgga aaacatggaa aacaaccaat ccattccctt tgctgatata
     3241 acaggctcca aagccaaaac ctgtcactgg aggctcaaga gcagatctcc agccaagagg
     3301 caaaggaatg ggggaagctg gagggcctcc ctctggttat ccaggcttct gaaggttcaa
     3361 gcaaagaaag ggttacaacc ttaaaaggag agcgtcccgg ggtatgggta gaagactgct
     3421 ccaccccgac ccccagggtc cctaaccgtc ttttccctgg gcgagtcagc ccaatcacag
     3481 gactgagagt gcctctttag tagcagcaag ccacttcgga cacccaaatg gaacacctcc
     3541 agtcagccct cgccgaccac cccaccccct ccatcctttt ccctcagcct ccgattggct
     3601 gaatctagag tccctccctg ctcccccctc tctccccacc cctggtgaaa actgcgggct
     3661 tcagcgctgg gtgcagcaac tggaggcgtt ggcgcaccag gaggaggctg cagctagggg
     3721 agtccaggtg agagcaggcc gacgggaggg acccgcacat gcaaggaccg ccgcagggcg
     3781 aggatgcaag ccttccccag ctacagtttt gggaaaggat accagggcgc tcctatatgg
     3841 gggcgcggga actggggaaa gaaggtgctc ccaggtcgag gtgggagagg aaggcagtgc
     3901 ggggtcacgg gctttctccc tgctaacgga cgctttcgaa gagtgggtgc cggaggagaa
     3961 ccatgaggaa ggacatcaag gacagccttt ggtccccaag ctcaaatcgc tttagtggtg
     4021 cgaatagagg gaggaggtgg gtggcaaact ggagggagtc cccagcgggt gacctcgtgg
     4081 ctggctgggt gcggggcacc gcaggtaaga aaaccgcaat gttgcgggag gggactgggt
     4141 ggcaggcgcg ggggagggga aagctagaaa ggatgcgagg gagcggaggg gggagggagc
     4201 gggagaatct caactggtag aggaagatta aaatgaggaa atagcatcag ggtggggtta
     4261 gccaagccgg gcctcaggga aagggcgcaa agtttgtctg ggtgtgggct taggtgggct
     4321 gggtatgaga ttcggggcgc cgaaaacact gctgcgcctc tgccaaatca cgctacccct
     4381 gtatctagtt ctgccaggct tctccagccc cagccccaat tcttttctct agtgttcccc
     4441 cttccctccc ctgaatctca agcccacact ccctcctcca taacccactg ttatcaaatc
     4501 taagtcattt gccacccaac aaccatcagg aggcggaagc agacgggagg agtttgagat
     4561 caacttgggc tacatcacga gttccaggct caccaaggct tcttaaggag accttgtctc
     4621 taaaattaat taattaatta attaatagtc ccctttctct gccacagaac cttgggatct
     4681 ggctcctggt cgcagctccc cccaccccag gctgacattc actgccatag cccatccgga
     4741 aatcctagtc tatttcccca tggatcttga actgcagaga gaatggcaga gtggcccgcc
     4801 ctgtgcaaag gatgttccta gcctaggtgg agctcgcgaa ctcgcagact gtgcctctct
     4861 tgggcaagga caggctagac agcctgccgg tgtgttgagc tagggcactg tggggaaggc
     4921 agagaacctg tgcagggcag caatgaacac aggaccagaa aactgcagcc ctaggaacac
     4981 tcaagagctg gccatttgca agcatctctg gcctccgtgc ttctcactca tgtcccatgt
     5041 cttatacagg cctctgtggc acctcgcttg cctgatctca tccctagccg ttaagctttc
     5101 tgcatgactt atcacttggg gcataatgct ggatacctac cattttctta gaccccatca
     5161 aaatcctatt tgagtgtacg gttcggagaa cctcatttat ccggtaaatg tcttttactc
     5221 tgctctcagg gagctgaggc aggacatcct gagatacatt gggagaggag atacagtttc
     5281 aataaaataa taggttgggt ggaggtacat gcctataatg ccaccactca ggaaatggtg
     5341 gcagcttcgt gagtttgagg ccaacccaag aaacatagtg aaaccctgtc agtaaataag
     5401 taagcaagta tttgagtatc tactatatgc tagggctgac ctggacatta ggggtcatct
     5461 tctgaacaaa ctagtgcttg agggaggtat ttggggtttt tgtttgttta atggatctga
     5521 atgagttcca gagactggct acacagcgat atgactgagc ttaacacccc taaagcatac
     5581 agtcagacca attagacaat aaaaggtatg tatagcttac caaataaaaa aattgtattt
     5641 tcaagagagt gtctgtctgt gtagccctgg ctgttcttga actcactctg tagaccaggc
     5701 tggcctggaa atccatctgc ctgcctctgc ctctctgcct ctctgcctct ctgcctctct
     5761 ctctgcctct ctctgcctct ctctgcccct ctctgcccct ctctgcccct ctctgccgcc
     5821 ctctgccttt tgccctctgc cctctgttct ctggcctctg ccctctgccc tctggcctct
     5881 ggcctctgcc tctgcctctt gagtgctgga atcaaaggtg tgagctctgt aggtcttaag
     5941 ttccagaaga aagtaatgaa gtcacccagc agggaggtgc tcagggacag cacagacaca
     6001 cacccaggac ataggctccc acttccttgg ctttctctga gtggcaaagg accttaggca
     6061 gtgtcactcc ctaagagaag gggataaaga gaggggctga ggtattcatc atgtgctccg
     6121 tggatctcaa gccctcaagg taaatgggga cccacctgtc ctaccagctg gctgacctgt
     6181 agctttcccc accacagaat ccaagtcgga actcttggca cctagaggat ctcgaatcac
     6241 aagtttgtac aaaaaagcag gctttaaagg aacgcacgta actataacgg tcctaaggta
     6301 gcgaacctag gataacttcg tatagcatac attatacgaa gttatcggcg cgcataactt
     6361 cgtataggat actttatacg aagttatcaa gcgctataac ttcgtatagt ataccttata
     6421 cgaagttatc tgatatcgcc accatggctc ctaagaagaa gaggaaggtg atgagcagcg
     6481 gcgccctgct gttccacggc aagatcccct acgtggtgga gatggagggc aatgtggatg
     6541 gccacacctt cagcatccgc ggcaagggct acggcgatgc cagcgtgggc aaggtggatg
     6601 cccagttcat ctgcaccacc ggcgatgtgc ccgtgccctg gagcaccctg gtgaccaccc
     6661 tgaccgcagg cgcccagtgc ttcgccaagt acggccccga gctgaaggat ttctacaaga
     6721 gctgcatgcc cgatggctac gtgcaggagc gcaccatcac cttcgagggt gatggcaatt
     6781 tcaagacccg cgccgaggtg accttcgaga atggcagcgt gtacaatcgc gtgaagctga
     6841 atggccaggg cttcaagaag gatggccacg tgctgggcaa gaatctggag ttcaatttca
     6901 ccccccactg cctgtacatc tggggcgatc aggccaatca cggcctgaag agcgccttca
     6961 agatctgcca cgagatcacc ggcagcaagg gcgatttcat cgtggccgat cacacccaga
     7021 tgaatacccc catcggcggc ggccccgtgc acgtgcccga gtaccaccac atgagctacc
     7081 acgtgaagct gagcaaggat gtgaccgatc accgcgataa tatgagcctg aaggagaccg
     7141 tgcgcgccgt ggattgccgc aagacctacc tgtgaagctt aattagctga gcttggactc
     7201 ctgttgatag atccagtaat gacctcagaa ctccatctgg ccgcgactct agatcataat
     7261 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     7321 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
     7381 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     7441 ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa ggcgtgctag cataacttcg
     7501 tatagcatac attatacgaa gttatctaac gttgccacca tggtgagcaa gggcgaggag
     7561 aataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcgcatgga gggctccgtg
     7621 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggctttcag
     7681 accgctaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc
     7741 cctcatttca cctacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac
     7801 ttcaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaacta cgaggacggc
     7861 ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg
     7921 aagctgcgcg gcaccaactt cccctccgac ggccccgtga tgcagaagaa gaccatgggc
     7981 tgggaggcct cctccgagcg gatgtacccc gaggacggtg ccctgaaggg caagatcaag
     8041 atgaggctga agctgaagga cggcggccac tacacctccg aggtcaagac cacctacaag
     8101 gccaagaagc ccgtgcagct gcccggcgcc tacatcgtcg acatcaagtt ggacatcacc
     8161 tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc
     8221 accggcggca tggacgagct gtacaagagt gcacgcgtga gtaagctgaa ccctcctgat
     8281 gagagtggcc ccggctgcat gagctgcaag tgtgtgctct cctgagcggc cgctaatcaa
     8341 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     8401 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     8461 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     8521 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     8581 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     8641 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     8701 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     8761 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     8821 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     8881 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgagac
     8941 tgctgcacca gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
     9001 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
     9061 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     9121 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaag
     9181 gcgtgtttaa acatataact tcgtatagga tactttatac gaagttatcg cgtacggcca
     9241 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     9301 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     9361 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     9421 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     9481 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     9541 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     9601 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     9661 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     9721 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     9781 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     9841 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     9901 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaaga
     9961 gtgcacgcgt gagtaagctg aaccctcctg atgagagtgg ccccggctgc atgagctgca
    10021 agtgtgtgct ctcctgagcg gccgctaatc aacctctgga ttacaaaatt tgtgaaagat
    10081 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
    10141 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
    10201 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
    10261 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
    10321 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
    10381 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
    10441 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
    10501 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
    10561 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
    10621 gggccgcctc cccgcatcga taccgtcgag actgctgcac cagatcataa tcagccatac
    10681 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
    10741 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
    10801 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
    10861 tggtttgtcc aaactcatca atgtatctta aggcgtgaag atcttcatat aacttcgtat
    10921 agtatacctt atacgaagtt atcaagtact gccaccatgg tgagcgagct gattaaggag
    10981 aacatgcaca tgaagctgta catggagggc accgtgaaca accaccactt caagtgcaca
    11041 tccgagggcg aaggcaagcc ctacgagggc acccagacca tgagaatcaa ggcggtcgag
    11101 ggcggccctc tccccttcgc cttcgacatc ctggctacca gcttcatgta cggcagcaaa
    11161 accttcatca accacaccca gggcatcccc gacttcttta agcagtcctt ccccgagggc
    11221 ttcacatggg agagagtcac cacatacgaa gacgggggcg tgctgaccgc tacccaggac
    11281 accagcctcc aggacggctg cctcatctac aacgtcaaga tcagaggggt gaacttccca
    11341 tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg aggcctccac cgagaccctg
    11401 taccccgctg acggcggcct ggaaggcaga gccgacatgg ccctgaagct cgtgggcggg
    11461 ggccacctga tctgcaactt gaagaccaca tacagatcca agaaacccgc taagaacctc
    11521 aagatgcccg gcgtctacta tgtggacaga agactggaaa gaatcaagga ggccgacaaa
    11581 gagacctacg tcgagcagca cgaggtggct gtggccagat actgcgacct ccctagcaaa
    11641 ctggggcaca gaagtgcacg cgtgagtaag ctgaaccctc ctgatgagag tggccccggc
    11701 tgcatgagct gcaagtgtgt gctctcctga gcggccgcta atcaacctct ggattacaaa
    11761 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
    11821 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
    11881 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
    11941 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
    12001 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
    12061 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
    12121 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
    12181 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
    12241 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
    12301 cggatctccc tttgggccgc ctccccgcat cgataccgtc gagactgctg caccagatca
    12361 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
    12421 ccctgaacct gaaacataaa atgaatgsma ttctagttgt ggtnttgtcc aaactcatca
    12481 atgtatyytw argsskwmyw rkkrarktyc ywwtyyyywa raargtatag gaacttccgt
    12541 aactataacg gtcctaaggt agcgaatgca tctagaccca gctttcttgt acaaagtggt
    12601 gattcgaggt ccttcctctg cagaggtctt gcttctcccg gtcagctgac tccctcccca
    12661 agtccttcaa atatctcaga acatggggag aaacggggac cttgtccctc ctaaggaacc
    12721 ccagtgctgc atgccatcat cccccccmcc cycscccccm cccccgccac ttctccctcc
    12781 atgcatacca ctagctgtca ttttgtactc tgtatttatt ccagggctgc ttctgattat
    12841 ttagtttgtt ctttccctgg agacctgtta gaacataagg gcgtatggtg ggtaggggag
    12901 gcaggatatc agtccctggg gcgagttcct ccctgccaac caagccagat gcctgaaaga
    12961 gatatggatg agggaagttg gactgtgcct gtacctggta cagtcatact ctgttgaaag
    13021 aatcatcggg gagggggggg ggctcaagag gggagagctc tgctgagcct ttgtggacca
    13081 tccaatgagg atgagggctt agattctacc aggtcattct cagccaccac acacaagcgc
    13141 tctgccatca ctgaagaagc cccctagggc tcttgggcca gggcacactc agtaaagatg
    13201 caggttcagt cagggaatga tggggaaagg ggtaggaggt gggggaggga tcaccccctc
    13261 ctctaaaaca cgagcctgct gtctccaaag gcctctgcct gtagtgaggg tggcagaaga
    13321 agacaaggag ccagaactct gactccagga tctaagtccg tgcaggaagg ggatcctaga
    13381 accatctggt tggacccagc ttaccaaggg agagccttta ttcttctttc ccttgcccct
    13441 ctgtgccagc ccctcttgct gtccctgatc ccccagacag cgagagtctt gcaacctgcc
    13501 tcttccaaga cctcctaatc tcaggggcag gcggtggagt gagatccggc gtgcacactt
    13561 tttggaagat agctttccca aggatcctct cccccactgg cagctctgcc tgtcccatca
    13621 ccatgtataa taccaccact gctacagcat ctcaccgagg aaagaaaact gcacaataaa
    13681 accaagcctc tggagtgtgt cctggtgtct gtctcttctg tgtcctggcg tctgtctctt
    13741 ctgtgttctt ccaaggtcag aaacaaaaac cacacacttc aacctggatg gctcggctga
    13801 gcacttctgt gtgcagaagg tccaaccaga ctctggggta ccccggccct ccctattccc
    13861 ttgcctcctg tctcccgctt tttatagctc cctatgctgg gcttctctgg agagtgaaat
    13921 ctttgcccaa atcaatgcgc attctctctg ctgagtcatc tggcgacagc agttgagttc
    13981 acccgccaac acatgggccc agctatgtag ccgaaccctg gctctggaag tgccagggac
    14041 tttgtgcata agtatgtacc atgccctttt ttcacagtcc tagctctgca gaagtgcagc
    14101 ctgaaggcct gtctgctgag aggacatgcc ctggagccct gaaacaggca cagtgggagg
    14161 aggaacggag gatgacaggc atcaggccct cagtccaaaa gcaaccactt gagaatgggc
    14221 tggagtacga aacatggggt cccgtccctg gatccctcct caaagagtaa taagtaaaat
    14281 ataaacaggt accccaggcc gttctgggtt tgggttgtaa tgggatccat ttgcagagaa
    14341 ctattgagac agcccagccg tactgtgaca ggcaatgtgg gggaggaggt tgaatcactt
    14401 ggtatttagc atgaatagaa taattccctg aacatttttc ttaaacatcc atatctaaat
    14461 taccaccact cgctcccagt cttcctgcct ttgcgccagc ctcctgtctg gccatgcctg
    14521 aagaaggctg gagaagccac ccacctcagg ccatgacact gccagccact tggcaggtgc
    14581 agccaaacct gagctgtccc agaaagggac attctcaaga cccaggcacc ctgatcagca
    14641 ctgacttgga gctacaagtg tcatgccaga aaagtctcta agaaaacctt ttcagggaaa
    14701 agggggtgac tcaacaccgg gcaagtttgg gaagccccac ccttcgagtg atggaagagc
    14761 agataggaag cctcagaaga gagacaccgg cacccaggta acgttcctca tgtggtctct
    14821 gtcacacggt cgactctctt aaggtagctc gaccgtgctc ttccctggac atctccgtga
    14881 ccacactctc agttcttagg gagatgcggg tgctctctga ggctatctca gagttgcaga
    14941 ttctgaggcc tagagtgact acagtcagcc taggaagcca cagaggactg tggaccagga
    15001 gggcagaaga ggagaaggga agaaaaacca tcagatagga cttgcaatga aactaaccca
    15061 agacaatcat aatgcagaca ggaatgttaa aggcgttcag cagctggcca tgacacccat
    15121 ctgtccctct ggccaagtca gcaagcctgg aagacctggg actcctgccc atatgtccta
    15181 agctccccac ccacccactc gttcactgtc cttattctct ctctaccttc agccacttag
    15241 tttcctacct taagtcctag aattgatcct ggcgtaatag cgaagaggcc cgcaccgatc
    15301 gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg tattttctcc
    15361 ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg
    15421 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
    15481 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
    15541 gtcagaggtt ttcaccgtca tcaccgaaac gcgcga
//
