LOCUS       sequence-222152-       13559 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid encoding the mitochondrial localization of the FRET 
            semi-synthetic biosensor measuring the ratio NADPH/NADP+ in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 13559)
  AUTHORS   Sallin O, Reymond L, Gondrand C, Raith F, Koch B, Johnsson K
  TITLE     Semisynthetic biosensors for mapping cellular concentrations of 
            nicotinamide adenine dinucleotides.
  JOURNAL   eLife. 2018 May 29;7. pii: 32638. doi: 10.7554/eLife.32638.
  PUBMED    29809136
REFERENCE   2  (bases 1 to 13559)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2018-05-29"; volume: "7. pii: 32638. doi"; pages: " 
            10.7554/eLife.32638"
COMMENT     Sequence Label: pEBTet_NADP-Snifit-mito
FEATURES             Location/Qualifiers
     source          1..13559
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(720..738)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        806..910
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(911..979,980..1771)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1129..1148)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1942..2530
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2431..2450
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(2719..2739)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2737..3034
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3111..3710
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3111..3130)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3607..3627
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    3801..3935
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4073..4092)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        4369..4630
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     intron          4747..5319
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     5327..5346
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     5374..5393
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5374..5392
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5403..6023
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(5476..5495)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     polyA_signal    6065..6186
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        6241..6620
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6621..6824
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6775..6795
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    6826..6844
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6847..6865
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     6875..6899
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             6996..7070
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             7083..7154
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="SVLTPLLLRGLTGSARRLPVPRAK"
     CDS             7173..7244
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="SVLTPLLLRGLTGSARRLPVPRAK"
     CDS             7257..7328
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="SVLTPLLLRGLTGSARRLPVPRAK"
     CDS             8151..9035
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEISG"
     primer_bind     complement(9766..9783)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9772..9883
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 cgctcctgct cctgctcctg ttccaccgtg ggtccctttg cagccaatgc aacttggacg
       61 tttttggggt ctccggacac catctctatg tcttggccct gatcctgagc cgcccggggc
      121 tcctggtctt ccgcctcctc gtcctcgtcc tcttccccgt cctcgtccat ggttatcacc
      181 ccctcttctt tgaggtccac tgccgccgga gccttctggt ccagatgtgt ctcccttctc
      241 tcctaggcca tttccaggtc ctgtacctgg cccctcgtca gacatgattc acactaaaag
      301 agatcaatag acatctttat tagacgacgc tcagtgaata cagggagtgc agactcctgc
      361 cccctccaac agccccccca ccctcatccc cttcatggtc gctgtcagac agatccaggt
      421 ctgaaaattc cccatcctcc gaaccatcct cgtcctcatc accaattact cgcagcccgg
      481 aaaactcccg ctgaacatcc tcaagatttg cgtcctgagc ctcaagccag gcctcaaatt
      541 cctcgtcccc ctttttgctg gacggtaggg atggggattc tcgggacccc tcctcttcct
      601 cttcaaggtc accagacaga gatgctactg gggcaacgga agaaaagctg ggtgcggcct
      661 gtgaggatca gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg
      721 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
      781 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
      841 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      901 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
      961 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     1021 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     1081 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     1141 ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     1201 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     1261 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     1321 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     1381 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     1441 caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     1501 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     1561 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     1621 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     1681 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     1741 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     1801 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     1861 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     1921 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1981 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     2041 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     2101 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     2161 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     2221 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     2281 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     2341 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     2401 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     2461 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     2521 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttg
     2581 aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat
     2641 cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcga
     2701 actagatgat ccggctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     2761 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     2821 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     2881 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     2941 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     3001 agctattcca gaagtagtga ggaggctttt ttggagggtg accgccacga ccggtgccgc
     3061 caccatcccc tgacccacgc ccctgacccc tcacaaggag acgaccttcc atgaccgagt
     3121 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc ccgggccgta cgcaccctcg
     3181 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgaccccgac cgccacatcg
     3241 aacgcgtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg
     3301 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag
     3361 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg
     3421 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt
     3481 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg
     3541 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg
     3601 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg
     3661 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga cgcccgcccc
     3721 acgacccgca gcgcccgacc gaaaggagcg cacgacccgg tccgacgcgg cccgacgggt
     3781 ccgagggggg tcgacctcga aacttgttta ttgcagctta taatggttac aaataaagca
     3841 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     3901 ccaaactcat caatgtatct tatcatgtct ggatctgatc cgaacccctt cctcgaccaa
     3961 ttctcatgtt tgacagctta tcatcgcaga tccgggcaac gttgttgcca ttgctcgacc
     4021 aattctcatg tttgacagct tatcatcgca gatccgtatg gtgcactctc agtacaatct
     4081 gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg
     4141 agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga
     4201 agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc
     4261 gtatctgagg ggactagggt gtgtttaggc gaaaagcggg gcttcggttg tacgcggtta
     4321 ggagtcccct caggatatag tagtttcgct tttgcatagg gagggggaaa tgtagtctta
     4381 tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg
     4441 agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt
     4501 aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattcc gcattgcaga
     4561 gatattgtat ttaagtgcct agctcgatac aataaacgcc atttgaccat tcaccacatt
     4621 ggtgtgcacc tccaagctgg ccggggatcc tctagggcct ctgagctatt ccagaagtag
     4681 tgaagaggct tttttggagg cctaggcttt tgcaaaaagc tccggatcga tcctgagaac
     4741 ttcagggtga gtttggggac ccttgattgt tctttctttt tcgctattgt aaaattcatg
     4801 ttatatggag ggggcaaagt tttcagggtg ttgtttagaa tgggaagatg tcccttgtat
     4861 caccatggac cctcatgata attttgtttc tttcactttc tactctgttg acaaccattg
     4921 tctcctctta ttttcttttc attttctgta actttttcgt taaactttag cttgcatttg
     4981 taacgaattt ttaaattcac ttttgtttat ttgtcagatt gtaagtactt tctctaatca
     5041 cttttttttc aaggcaatca gggtatatta tattgtactt cagcacagtt ttagagaaca
     5101 attgttataa ttaaatgata aggtagaata tttctgcata taaattctgg ctggcgtgga
     5161 aatattctta ttggtagaaa caactacatc ctggtcatca tcctgccttt ctctttatgg
     5221 ttacaatgat atacactgtt tgagatgagg ataaaatact ctgagtccaa accgggcccc
     5281 tctgctaacc atgttcatgc cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt
     5341 attgtgctgt ctcatcattt tggcaaagaa ttgtaatacg actcactata gggcgaattg
     5401 atatgtctag attagataaa agtaaagtga ttaacagcgc attagagctg cttaatgagg
     5461 tcggaatcga aggtttaaca acccgtaaac tcgcccagaa gctaggtgta gagcagccta
     5521 cattgtattg gcatgtaaaa aataagcggg ctttgctcga cgccttagcc attgagatgt
     5581 tagataggca ccatactcac ttttgccctt tagaagggga aagctggcaa gattttttac
     5641 gtaataacgc taaaagtttt agatgtgctt tactaagtca tcgcgatgga gcaaaagtac
     5701 atttaggtac acggcctaca gaaaaacagt atgaaactct cgaaaatcaa ttagcctttt
     5761 tatgccaaca aggtttttca ctagagaatg cattatatgc actcagcgct gtggggcatt
     5821 ttactttagg ttgcgtattg gaagatcaag agcatcaagt cgctaaagaa gaaagggaaa
     5881 cacctactac tgatagtatg ccgccattat tacgacaagc tatcgaatta tttgatcacc
     5941 aaggtgcaga gccagccttc ttattcggcc ttgaattgat catatgcgga ttagaaaaac
     6001 aacttaaatg tgaaagtggg tccgcgtaca gcggatcccg ggaattcaga tcttattaaa
     6061 gcagaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     6121 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     6181 atcttatcat gtctggtcga atccatggcg cagaactggt aggtatggaa gatccgcgtt
     6241 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     6301 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     6361 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     6421 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     6481 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     6541 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
     6601 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
     6661 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
     6721 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
     6781 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata
     6841 gagatctccc tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg
     6901 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc
     6961 tccggactct agcgtttaaa cttaagctta ccaccatgtc cgtcctgacg ccgctgctgc
     7021 tgcggggctt gacaggctcg gcccggcggc tcccagtgcc gcgcgccaag atccattcgt
     7081 tgtccgtcct gacgccgctg ctgctgcggg gcttgacagg ctcggcccgg cggctcccag
     7141 tgccgcgcgc caagatccat tcgttgggag gcagcgtgct caccccactc ctgctgcggg
     7201 gcttgacagg ctcggcccgg cggctcccag tgccgcgcgc taaaattcac agcctctccg
     7261 tcctgacgcc gctgctgctg cggggcttga caggctcggc ccggcggctc ccagtgccgc
     7321 gcgccaagat ccattcgttg ggaggcagcg gaggcagcga gggcgggctg gggcgtgctg
     7381 tgtgcttgct gaccggggcc tcccgcggct tcggccggac gctggccccg ctcctggcct
     7441 cgctgctgtc gcccggctcc gtgcttgtcc ttagcgcccg caacgacgag gcactgcgcc
     7501 agctggaggc cgagctgggc gccgagcggt ctggcctgcg cgtggtgcgg gtgcccgccg
     7561 acctgggcgc cgaggccggc ttgcagcagc tgctcggcgc cctgcgcgag ctcccccggc
     7621 ccaaggggct gcagcgactg ctgcttatca acaacgcggg ctctcttggg gatgtgtcca
     7681 aaggcttcgt ggacctgagt gactccactc aagtgaacaa ctactgggca ctgaacttga
     7741 cctccatgct ctgcctgact tccagcgtcc tgaaggcctt cccggacagt cctggcctca
     7801 acagaaccgt ggttaacatc tcgtccctct gtgccctgca acctttcaaa ggctgggcgc
     7861 tgtactgtgc aggaaaggct gctcgtgata tgctgttcca ggtcctggcg ctggaggaac
     7921 ctaatgtgag ggtgctgaac tatgccccag gtcctctgga cacagacatg cagcagttgg
     7981 cccgggagac ctccgtggac ccagacatgc gaaaagggct gcaggagctg aaggcaaagg
     8041 ggaagctggt ggattgcaag gtgtcagccc agaaactgct gagcttactg gaaaaggacg
     8101 agttcaagtc tggagcccac gtggacttct atgacaaaga attcggatcc gaaatcggta
     8161 ctggctttcc attcgacccc cattatgtgg aagtcctggg cgagcgcatg cactacgtcg
     8221 atgttggtcc gcgcgatggc acccctgtgc tgttcctgca cggtaacccg acctcctcct
     8281 acgtgtggcg caacatcatc ccgcatgttg caccgaccca tcgctgcatt gctccagacc
     8341 tgatcggtat gggcaaatcc gacaaaccag acctgggtta tttcttcgac gaccacgtcc
     8401 gcttcatgga tgccttcatc gaagccctgg gtctggaaga ggtcgtcctg gtcattcacg
     8461 actggggctc cgctctgggt ttccactggg ccaagcgcaa tccagagcgc gtcaaaggta
     8521 ttgcatttat ggagttcatc cgccctatcc cgacctggga cgaatggcca gaatttgccc
     8581 gcgagacctt ccaggccttc cgcaccaccg acgtcggccg caagctgatc atcgatcaga
     8641 acgtttttat cgagggtacg ctgccgatgg gtgtcgtccg cccgctgact gaagtcgaga
     8701 tggaccatta ccgcgagccg ttcctgaatc ctgttgaccg cgagccactg tggcgcttcc
     8761 caaacgagct gccaatcgcc ggtgagccag cgaacatcgt cgcgctggtc gaagaataca
     8821 tggactggct gcaccagtcc cctgtcccga agctgctgtt ctggggcacc ccaggcgttc
     8881 tgatcccacc ggccgaagcc gctcgcctgg ccaaaagcct gcctaactgc aaggctgtgg
     8941 acatcggccc gggtctgaat ctgctgcaag aagacaaccc ggacctgatc ggcagcgaga
     9001 tcgcgcgctg gctgtctact ctggagattt ccggtagcgg ccgccctcct cctccgccac
     9061 cgccaccacc gccgccgccg ccaccaccac ctcctccgcc tcctccacca ccaccgccac
     9121 cgcctccacc acctggcggc cgctctcggt ctctcgagat ggataaagac tgcgaaatga
     9181 aacgcactac cctggacagc ccgctgggta aactggaact gagcggttgc gagcagggcc
     9241 tgcacgaaat cattttcctg ggcaaaggta cgagcgctgc ggatgcggtc gaagtaccgg
     9301 ctccggctgc ggttctgggt ggcccggagc cgctgatgca ggctaccgca tggctgaacg
     9361 catattttca ccagccagag gcgattgaag agttccctgt accggccctg catcacccgg
     9421 tatttcagca ggaatccttt acccgtcagg tactgtggaa actgctgaaa gtagtgaaat
     9481 tcggcgaggt gatctcctat tcccatctgg cggcgctggc tggcaaccca gcggcaactg
     9541 cggcggtgaa aaccgctctg tctggtaatc cggtgccaat tctgatcccg tgccaccgtg
     9601 tggttcaggg cgacctggac gtaggtggct acgaaggcgg cctggctgtg aaagaatggc
     9661 tgctggcgca cgaaggtcac cgtctgggta aaccaggcct gggctaaagc tagcggcgcg
     9721 cccggccgct cgagtctaga gggcccgttt aaacccgctg atcagcctcg actgtgcctt
     9781 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     9841 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     9901 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     9961 atagcaggct agaggatcga tccccgcccc ggacgaacta aacctgacta cgacatctct
    10021 gccccttctt cgcggggcag tgcatgtaat cccttcagtt ggttggtaca acttgccaac
    10081 tgggccctgt tccacatgtg acacgggggg ggaccaaaca caaaggggtt ctctgactgt
    10141 agttgacatc cttataaatg gatgtgcaca tttgccaaca ctgagtggct ttcatcctgg
    10201 agcagacttt gcagtctgtg gactgcaaca caacattgcc tttatgtgta actcttggct
    10261 gaagctctta caccaatgct gggggacatg tacctcccag gggcccagga agactacggg
    10321 aggctacacc aacgtcaatc agaggggcct gtgtagctac cgataagcgg accctcaaga
    10381 gggcattagc aatagtgttt ataaggcccc cttgttaacc ctaaacgggt agcatatgct
    10441 tcccgggtag tagtatatac tatccagact aaccctaatt caatagcata tgttacccaa
    10501 cgggaagcat atgctatcga attagggtta gtaaaagggt cctaaggaac agcgatatct
    10561 cccaccccat gagctgtcac ggttttattt acatggggtc aggattccac gagggtagtg
    10621 aaccatttta gtcacaaggg cagtggctga agatcaagga gcgggcagtg aactctcctg
    10681 aatcttcgcc tgcttcttca ttctccttcg tttagctaat agaataactg ctgagttgtg
    10741 aacagtaagg tgtatgtgag gtgctcgaaa acaaggtttc aggtgacgcc cccagaataa
    10801 aatttggacg gggggttcag tggtggcatt gtgctatgac accaatataa ccctcacaaa
    10861 ccccttgggc aataaatact agtgtaggaa tgaaacattc tgaatatctt taacaataga
    10921 aatccatggg gtggggacaa gccgtaaaga ctggatgtcc atctcacacg aatttatggc
    10981 tatgggcaac acataatcct agtgcaatat gatactgggg ttattaagat gtgtcccagg
    11041 cagggaccaa gacaggtgaa ccatgttgtt acactctatt tgtaacaagg ggaaagagag
    11101 tggacgccga cagcagcgga ctccactggt tgtctctaac acccccgaaa attaaacggg
    11161 gctccacgcc aatggggccc ataaacaaag acaagtggcc actctttttt ttgaaattgt
    11221 ggagtggggg cacgcgtcag cccccacacg ccgccctgcg gttttggact gtaaaataag
    11281 ggtgtaataa cttggctgat tgtaaccccg ctaaccactg cggtcaaacc acttgcccac
    11341 aaaaccacta atggcacccc ggggaatacc tgcataagta ggtgggcggg ccaagatagg
    11401 ggcgcgattg ctgcgatctg gaggacaaat tacacacact tgcgcctgag cgccaagcac
    11461 agggttgttg gtcctcatat tcacgaggtc gctgagagca cggtgggcta atgttgccat
    11521 gggtagcata tactacccaa atatctggat agcatatgct atcctaatct atatctgggt
    11581 agcataggct atcctaatct atatctgggt agtatatgct atcctaatct gtatccgggt
    11641 agcatatgct atcctaatag agattagggt agtatatgct atcctaattt atatctgggt
    11701 agcatatact acccaaatat ctggatagca tatgctatcc taatctatat ctgggtagca
    11761 tatgctatcc taatctatat ctgggtagca taggctatcc taatctatat ctgggtagca
    11821 tatgctatcc taatctatat ctgggtagta tatgctatcc taatttatat ctgggtagca
    11881 taggctatcc taatctatat ctgggtaggc atatgctatc ctaatctata tctgggtagc
    11941 atatgctatc ctaatctata tctgggtagc ataggctatc ctaatctata tctgggtagc
    12001 atatgctatc ctaatctata tctgggtagt atatgctatc ctaatttata tctgggtagc
    12061 ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt
    12121 atatgctatc ctaatctgta tccgggtagc atatgctatc ctcatgcata tacagtcagc
    12181 atatgatacc cagtagtaga gtgggagtgc tatcctttgc atatgccgcc acctcccaag
    12241 ggggcgtgaa ttttcgctgc ttgtcctttt cctgctggtt gctcccattc ttaggtgaat
    12301 ttaaggaggc caggctaaag ccgtcgcatg tctgattgct caccaggtaa atgtcgctaa
    12361 tgttttccaa cgcgagaagg tgttgagcgc ggagctgagt gacgtgacaa catgggtatg
    12421 cccaattgcc ccatgttggg aggacgaaaa tggtgacaag acagatggcc agaaatacac
    12481 caacagcacg catgatgtct actggggatt tattctttag tgcgggggaa tacacggctt
    12541 ttaatacgat tgagggcgtc tcctaacaag ttacatcact cctgcccttc ctcaccctca
    12601 tctccatcac ctccttcatc tccgtcatct ccgtcatcac cctccgcggc agccccttcc
    12661 accataggtg gaaaccaggg aggcaaatct actccatcgt caaagctgca cacagtcacc
    12721 ctgatattgc aggtaggagc gggctttgtc ataacaaggt ccttaatcgc atccttcaaa
    12781 acctcagcaa atatatgagt ttgtaaaaag accatgaaat aacagacaat ggactccctt
    12841 agcgggccag gttgtgggcc gggtccaggg gccattccaa aggggagacg actcaatggt
    12901 gtaagacgac attgtggaat agcaagggca gttcctcgcc ttaggttgta aagggaggtc
    12961 ttactacctc catatacgaa cacaccggcg acccaagttc cttcgtcggt agtcctttct
    13021 acgtgactcc tagccaggag agctcttaaa ccttctgcaa tgttctcaaa tttcgggttg
    13081 gaacctcctt gaccacgatg ctttccaaac caccctcctt ttttgcgcct gcctccatca
    13141 ccctgacccc ggggtccagt gcttgggcct tctcctgggt catctgcggg gccctgctct
    13201 atcgctcccg ggggcacgtc aggctcacca tctgggccac cttcttggtg gtattcaaaa
    13261 taatcggctt cccctacagg gtggaaaaat ggccttctac ctggaggggg cctgcgcggt
    13321 ggagacccgg atgatgatga ctgactactg ggactcctgg gcctcttttc tccacgtcca
    13381 cgacctctcc ccctggctct ttcacgactt ccccccctgg ctctttcacg tcctctaccc
    13441 cggcggcctc cactacctcc tcgaccccgg cctccactac ctcctcgacc ccggcctcca
    13501 ctgcctcctc gaccccggcc tccacctcct gctcctgccc ctcctgctcc tgcccctcc
//
