LOCUS       sequence-22219-e         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   He W, Dorn DC, Erdjument-Bromage H, Tempst P, Moore MA, Massague J
  TITLE     Hematopoiesis controlled by distinct TIF1gamma and Smad4 branches of
            the TGFbeta pathway.
  JOURNAL   Cell. 2006 Jun 2. 125(5):929-41.
  PUBMED    16751102
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2006 
            Jun 2. 125(5):929-41."
COMMENT     Sequence Label: pRetroSuper-GFP Smad2
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(87..103)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(88..104)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             complement(118..534)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(134..150)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(166..188)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 tctgtatgag acacagatcc ccggattgcc acatgttata tttcaagaga atataacatg
       61 tggcaatcct ttttggaaaa gcttatcgat accgtcgacc tcgagagatc taattccggc
      121 gcctagagaa ggagtgaggg ctggataaag ggaggatcga ggcggggtcg aacgaggagg
      181 ttcaaggggg agagacgggg cggatggagg aagaggaggc ggaggcttag ggtgtacaaa
      241 gggcttgacc cagggagggg ggtcaaaagc caaggcttcc caggtcacga tgtaggggac
      301 ctggtctggg tgtccatgcg ggccaggtga aaagaccttg atcttaacct gggtgatgag
      361 gtctcggtta aaggtgccgt ctcgcggcca tccgacgtta aaggttggcc attctgcaga
      421 gcagaaggta acccaacgtc tcttcttgac atctaccgac tggttgtgag cgatccgctc
      481 gacatctttc cagtgaccta aggtcaaact taagggagtg gtaacagtct ggccctaatt
      541 ttcagacaaa tacagaaaca cagtcagaca gagacaacac agaacgatgc tgcagcgctg
      601 cagcagacaa gacgcgcggc ttcggttcca aaccgaaagc aaaaattcag acggaggcgg
      661 gaactgtttt aggttctcgt ctcctaccag aaccacatat cctgacgggg tcggattcca
      721 catcgactcc cttcctcagg tcgggccaca aaaacggccc ccaaagtccc tgggacgtct
      781 cccagggttg cggccgggtg ttcagaactc gtcagttcca ccacgggtcc gccagataca
      841 gagctagtta gctaactagt acagacgcag gcgcaaacat tagatgccgg cacaaa
//
