LOCUS       sequence-222208-        9493 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive lentiviral expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9493)
  AUTHORS   Li J, Choi PS, Chaffer CL, Labella K, Hwang JH, Giacomelli AO, Kim 
            JW, Ilic N, Doench JG, Ly SH, Dai C, Hagel K, Hong AL, Gjoerup O, 
            Goel S, Ge JY, Root DE, Zhao JJ, Brooks AN, Weinberg RA, Hahn WC
  TITLE     An alternative splicing switch in FLNB promotes the mesenchymal cell
            state in human breast cancer.
  JOURNAL   Elife. 2018 Jul 30;7. pii: 37184. doi: 10.7554/eLife.37184.
  PUBMED    30059005
REFERENCE   2  (bases 1 to 9493)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-07-30"; volume: "7. pii: 37184. doi"; pages: " 
            10.7554/eLife.37184"
COMMENT     Sequence Label: pLX317-QKI
FEATURES             Location/Qualifiers
     source          1..9493
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2247..2271)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2279..2320
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2431..3019
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2484..2504)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2902..2913)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3091..3324
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3402..3523
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3439..3458)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3493..3512
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3563..3698
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3718..3737)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3719..3737)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3747..3764)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3747..3763)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3756..3778)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3905..4360
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3992..4011)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4202..4223
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4386..4490
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4491..4559,4560..5351)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4709..4728)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5522..6110
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6011..6030
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6264..6281
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6398..6419
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6434..6439,6440..6457,6458..6464)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6472..6488
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6477..6499
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6496..6512
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6496..6512
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6531..6551
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6533..6551
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6579..6805
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6806..6986
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7033..7158
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7651..7884
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8069..8113
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8344..8554
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8366..8562
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8413..8548
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8594..9196
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8594..8613)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9090..9110
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9239..9356
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcatggtcgg ggaaatggaa acgaaggaga agccgaagcc
     1261 caccccagat tacctgatgc agctgatgaa cgacaagaag ctcatgagca gcctgcccaa
     1321 cttctgcggg atcttcaacc acctcgagcg gctgctggac gaagaaatta gcagagtacg
     1381 gaaagacatg tacaatgaca cattaaatgg cagtacagag aaaaggagtg cagaattgcc
     1441 tgatgctgtg ggacctattg ttcagttaca agagaaactt tatgtgcctg taaaagaata
     1501 cccagatttt aattttgttg ggagaatcct tggacctaga ggacttacag ccaaacaact
     1561 tgaagcagaa accggatgta aaatcatggt ccgaggcaaa ggctcaatga gggataaaaa
     1621 aaaggaggag caaaatagag gcaagcccaa ttgggagcat ctaaatgaag atttacatgt
     1681 actaatcact gtggaagatg ctcagaacag agcagaaatc aaattgaaga gagcagttga
     1741 agaagtgaag aaattattgg tacctgcagc agaaggagaa gacagcctga agaagatgca
     1801 gctgatggag cttgcgattc tgaatggcac ctacagagat gccaacatta aatcaccagc
     1861 ccttgccttt tctcttgcag caacagccca ggctgctcca aggatcatta ctgggcctgc
     1921 gccggttctc ccaccagctg ccctgcgtac tcctacgcca gctggcccta ccataatgcc
     1981 tttgatcaga caaatacaga ccgctgtcat gccaaacgga actcctcacc caactgctgc
     2041 aatagttcct ccagggcccg aagctggttt aatctataca ccctatgagt acccctacac
     2101 attggcacca gctacatcaa tccttgagta tcctattgaa cctagtggtg tattaggtgc
     2161 ggtggctact aaagttcgaa ggcacgatat gcgtgtccat ccttaccaaa ggattgtgac
     2221 cgcagaccga gccgccaccg gcaactaccc aactttcttg tacaaagtgg ttgatatcgg
     2281 taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgaa ctagtccgta
     2341 acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg acctgactcc
     2401 gtgtaccctt cacccacgcg ttaagtcgac aatcaacctc tggattacaa aatttgtgaa
     2461 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     2521 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     2581 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     2641 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     2701 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     2761 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     2821 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     2881 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     2941 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     3001 ctttgggccg cctccccgcg tcgactttaa gaccaatgac ttacaaggca gctgtagatc
     3061 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     3121 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     3181 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     3241 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     3301 agtcagtgtg gaaaatctct agcagtacgt atagtagttc atgtcatctt attattcagt
     3361 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     3421 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     3481 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     3541 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     3601 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     3661 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc
     3721 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
     3781 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     3841 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     3901 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     3961 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     4021 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     4081 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     4141 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     4201 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     4261 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     4321 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     4381 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     4441 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     4501 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     4561 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     4621 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     4681 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     4741 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     4801 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     4861 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     4921 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     4981 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     5041 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     5101 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     5161 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     5221 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     5281 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     5341 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     5401 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     5461 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     5521 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     5581 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     5641 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     5701 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     5761 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     5821 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     5881 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     5941 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     6001 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     6061 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     6121 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     6181 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     6241 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     6301 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     6361 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     6421 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     6481 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc
     6541 ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt
     6601 gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca
     6661 ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca
     6721 gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt
     6781 taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga
     6841 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     6901 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     6961 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
     7021 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
     7081 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
     7141 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
     7201 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
     7261 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
     7321 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
     7381 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
     7441 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
     7501 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
     7561 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
     7621 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
     7681 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
     7741 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
     7801 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
     7861 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
     7921 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
     7981 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
     8041 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
     8101 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
     8161 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
     8221 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
     8281 gggacccatg cattgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     8341 ccagcaggca gaagtatgca aagcatgcgt ctcaattagt cagcaaccat agtcccgccc
     8401 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     8461 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     8521 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctttc tagaggtacc
     8581 accggagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     8641 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     8701 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     8761 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgccg tggcggtctg
     8821 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     8881 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     8941 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     9001 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     9061 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     9121 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     9181 gcccggtgcc agttaacgaa ttctagatct tgagacaaat ggcagtattc atccacaatt
     9241 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     9301 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
     9361 tttattacag ggacagcaga gatccacttt ggcgccggct cgaggcctgc aggtgcaaag
     9421 atggataaag ttttaaacag agaggaatct ttgcagctaa tggaccttct aggtcttgaa
     9481 aggagtggga att
//
