LOCUS       sequence-222210-        3786 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid is part of the Minimal Antibiotic Resistance Platform 
            (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that 
            constitutively expresses genes using the Pbla promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Cox G, Sieron A, King AM, De Pascale G, Pawlowski AC, Koteva K, 
            Wright GD
  TITLE     A Common Platform for Antibiotic Dereplication and Adjuvant 
            Discovery.
  JOURNAL   Cell Chem Biol. 2017 Jan 19;24(1):98-109. doi: 
            10.1016/j.chembiol.2016.11.011. Epub 2016 Dec 22.
  PUBMED    28017602
REFERENCE   2  (bases 1 to 3786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2016.11.011"; journalName: "Cell Chem Biol"; 
            date: "2017-01-19- 19"; volume: "24"; issue: "1"; pages: "98-109"
COMMENT     Sequence Label: pGDP3 vatD
FEATURES             Location/Qualifiers
     source          1..3786
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             202..224
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             881..898
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      complement(960..987)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        1012..1116
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1117..1185,1186..1977)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1335..1354)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2148..2736
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2637..2656
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2890..2907
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2922..3061
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2987..3006)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3147..3169
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3163..3354)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 gatctggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
       61 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
      121 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
      181 aaatgcttct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggtccga
      241 atcctatgaa aatgtatcct atagaaggaa acaaatcagt acaatttatc aaacctattt
      301 tagaaaaatt agaaaatgtt gaggttggag aatactcata ttatgattct aagaatggag
      361 aaacttttga taagcaaatt ttatatcatt atccaatctt aaacgataag ttaaaaatag
      421 gtaaattttg ctcaatagga ccaggtgtaa ctattattat gaatggagca aatcatagaa
      481 tggatggctc aacatatcca tttaatttat ttggtaatgg atgggagaaa catatgccaa
      541 aattagatca actacctatt aagggggata caataatagg taatgatgta tggataggaa
      601 aagatgttgt aattatgcca ggagtaaaaa tcggggatgg tgcaatagta gctgctaatt
      661 ctgttgttgt aaaagatata gcgccataca tgttagctgg aggaaatcct gctaacgaaa
      721 taaaacaaag atttgatcaa gatacaataa atcagctgct tgatataaaa tggtggaatt
      781 ggccaataga cattattaat gagaatatag ataaaattct tgataatagc atcattagag
      841 aagtcatatg gaaaaaatga agcttgcggc cgcactcgag caccaccacc accaccactg
      901 agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca
      961 aaaggccatc cgtcaggatg gccttctggt ggcacttttc ggggaaatgt gcgcggaacc
     1021 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     1081 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     1141 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     1201 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     1261 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     1321 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     1381 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     1441 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     1501 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     1561 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     1621 gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg
     1681 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     1741 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     1801 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     1861 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     1921 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     1981 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     2041 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     2101 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     2161 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     2221 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     2281 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     2341 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     2401 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     2461 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     2521 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     2581 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     2641 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     2701 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     2761 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     2821 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     2881 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc
     2941 cttacgcatc tgtgcggtat ttcacaccgc aatggtgcac tctcagtaca atctgctctg
     3001 atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc
     3061 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     3121 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     3181 atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc
     3241 acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt
     3301 ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc
     3361 ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat
     3421 gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa
     3481 acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg
     3541 cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat
     3601 ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa
     3661 accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca
     3721 cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag
     3781 ccggga
//
