LOCUS       sequence-222211-        9922 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive lentiviral expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9922)
  AUTHORS   Li J, Choi PS, Chaffer CL, Labella K, Hwang JH, Giacomelli AO, Kim 
            JW, Ilic N, Doench JG, Ly SH, Dai C, Hagel K, Hong AL, Gjoerup O, 
            Goel S, Ge JY, Root DE, Zhao JJ, Brooks AN, Weinberg RA, Hahn WC
  TITLE     An alternative splicing switch in FLNB promotes the mesenchymal cell
            state in human breast cancer.
  JOURNAL   Elife. 2018 Jul 30;7. pii: 37184. doi: 10.7554/eLife.37184.
  PUBMED    30059005
REFERENCE   2  (bases 1 to 9922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-07-30"; volume: "7. pii: 37184. doi"; pages: " 
            10.7554/eLife.37184"
COMMENT     Sequence Label: pLX317-CELF4
FEATURES             Location/Qualifiers
     source          1..9922
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2676..2700)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2708..2749
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2860..3448
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2913..2933)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3331..3342)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3520..3753
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3831..3952
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3868..3887)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3922..3941
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3992..4127
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4147..4166)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4148..4166)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4176..4193)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4176..4192)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4185..4207)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4334..4789
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4421..4440)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4631..4652
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4815..4919
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4920..4988,4989..5780)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5138..5157)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5951..6539
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6440..6459
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6693..6710
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6827..6848
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6863..6868,6869..6886,6887..6893)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6901..6917
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6906..6928
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6925..6941
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6925..6941
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6960..6980
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6962..6980
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7008..7234
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7235..7415
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7462..7587
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8080..8313
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8498..8542
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8773..8983
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8795..8991
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8842..8977
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9023..9625
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9023..9042)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9519..9539
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9668..9785
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcatgtatat aaagatggcc acgttagcaa acggacaggc
     1261 tgacaacgca agcctcagta ccaacgggct cggcagcagc ccgggcagtg ccgggcacat
     1321 gaacggatta agccacagcc cggggaaccc gtcgaccatt cccatgaagg accatgatgc
     1381 catcaagctg ttcattgggc agatcccccg caacctggat gagaaggacc tcaagcccct
     1441 cttcgaggag tttggcaaaa tctacgagct tacggttctg aaggacaggt tcacaggcat
     1501 gcacaaaggc tgcgccttcc tcacctactg cgagcgtgag tcagcgctga aggcccagag
     1561 cgcgctgcac gagcagaaga ctctgcccgg gatgaaccgg ccgatccagg tgaagcctgc
     1621 ggacagcgag agccgaggag gtagtagctg cctgcgccag cccccttcac atagaaaact
     1681 cttcgtgggc atgctcaaca agcaacagtc cgaggacgac gtgcgccgcc ttttcgaggc
     1741 ctttgggaac atcgaggagt gcaccatcct gcgcgggccc gacggcaaca gcaaggggtg
     1801 cgcctttgtg aagtactcct cccacgccga ggcgcaggcc gccatcaacg cgctacacgg
     1861 cagccagacc atgccgggag cctcgtccag tctggtggtc aagttcgccg acaccgacaa
     1921 ggagcgcacg atgcggcgaa tgcagcagat ggctggccag atgggcatgt tcaaccccat
     1981 ggccatccct ttcggggcct acggcgccta cgctcaggca ctgatgcagc agcaagcggc
     2041 cctgatggca tcagtcgcgc agggcggcta cctgaacccc atggctgcct tcgctgccgc
     2101 ccagatgcag cagatggcgg ccctcaacat gaatggcctg gcggccgcac ctatgacccc
     2161 aacctcaggt ggcagcaccc ctccgggcat cactgcacca gccgtgccta gcatcccatc
     2221 ccccattggg gtgaatggct tcaccggcct ccccccacag gccaatgggc aacctgctgc
     2281 ggaagctgtg ttcgccaatg gcatccaccc ctacccagca cagagcccca ccgccgcgga
     2341 ccccctgcag caggcctacg ccggagtgca gcagtatgca gcagctgcct accctgctgc
     2401 ctatggtcag ataagccagg cctttcctca gccgcctcca atgatccccc agcagcagag
     2461 agaagggccc gagggctgta acctgttcat ctaccatctg ccccaggagt ttggggacgc
     2521 tgagctgatg cagatgttcc tccctttcgg cttcgtgagc ttcgacaacc cggccagcgc
     2581 gcagaccgcc atccaggcca tgaacggctt ccagatcggc atgaagaggc tcaaggtgca
     2641 gctgaagcgg cccaaagacg ccaatcgccc gtactgccca actttcttgt acaaagtggt
     2701 tgatatcggt aagcctatcc ctaaccctct cctcggtctc gattctacgt agtaatgaac
     2761 tagtccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga
     2821 tggtaactgt aacccgaggt ctcgacgcgt taagtcgaca atcaacctct ggattacaaa
     2881 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     2941 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     3001 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     3061 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     3121 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     3181 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     3241 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     3301 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     3361 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     3421 cggatctccc tttgggccgc ctccccgcgt cgactttaag accaatgact tacaaggcag
     3481 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     3541 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     3601 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     3661 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     3721 gaccctttta gtcagtgtgg aaaatctcta gcagtacgta tagtagttca tgtcatctta
     3781 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
     3841 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     3901 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     3961 ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     4021 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     4081 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc
     4141 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
     4201 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     4261 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     4321 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     4381 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     4441 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     4501 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     4561 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     4621 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     4681 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     4741 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
     4801 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     4861 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     4921 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     4981 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     5041 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     5101 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     5161 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     5221 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     5281 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     5341 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     5401 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     5461 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     5521 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     5581 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     5641 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     5701 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     5761 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     5821 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     5881 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     5941 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     6001 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     6061 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     6121 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     6181 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     6241 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     6301 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     6361 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     6421 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     6481 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     6541 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     6601 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     6661 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     6721 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     6781 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     6841 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     6901 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
     6961 caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc
     7021 aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga
     7081 gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg
     7141 aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga
     7201 tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg
     7261 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     7321 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     7381 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
     7441 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
     7501 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
     7561 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
     7621 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
     7681 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
     7741 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     7801 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     7861 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     7921 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     7981 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     8041 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     8101 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     8161 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     8221 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     8281 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     8341 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     8401 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     8461 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     8521 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     8581 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     8641 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     8701 aaccccgagg ggacccatgc attgcatctc aattagtcag caaccaggtg tggaaagtcc
     8761 ccaggctccc cagcaggcag aagtatgcaa agcatgcgtc tcaattagtc agcaaccata
     8821 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     8881 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     8941 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctttct
     9001 agaggtacca ccggagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg
     9061 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
     9121 gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt
     9181 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgccgt
     9241 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
     9301 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
     9361 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
     9421 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
     9481 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
     9541 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
     9601 gacccgcaag cccggtgcca gttaacgaat tctagatctt gagacaaatg gcagtattca
     9661 tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
     9721 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
     9781 attttcgggt ttattacagg gacagcagag atccactttg gcgccggctc gaggcctgca
     9841 ggtgcaaaga tggataaagt tttaaacaga gaggaatctt tgcagctaat ggaccttcta
     9901 ggtcttgaaa ggagtgggaa tt
//
