LOCUS       sequence-222212-        9262 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive lentiviral expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9262)
  AUTHORS   Li J, Choi PS, Chaffer CL, Labella K, Hwang JH, Giacomelli AO, Kim 
            JW, Ilic N, Doench JG, Ly SH, Dai C, Hagel K, Hong AL, Gjoerup O, 
            Goel S, Ge JY, Root DE, Zhao JJ, Brooks AN, Weinberg RA, Hahn WC
  TITLE     An alternative splicing switch in FLNB promotes the mesenchymal cell
            state in human breast cancer.
  JOURNAL   Elife. 2018 Jul 30;7. pii: 37184. doi: 10.7554/eLife.37184.
  PUBMED    30059005
REFERENCE   2  (bases 1 to 9262)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-07-30"; volume: "7. pii: 37184. doi"; pages: " 
            10.7554/eLife.37184"
COMMENT     Sequence Label: pLX317-SNAI1
FEATURES             Location/Qualifiers
     source          1..9262
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2016..2040)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2048..2089
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2200..2788
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2253..2273)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2671..2682)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2860..3093
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3171..3292
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3208..3227)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3262..3281
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3332..3467
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3487..3506)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3488..3506)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3516..3533)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3516..3532)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3525..3547)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3674..4129
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3761..3780)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3971..3992
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4155..4259
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4260..4328,4329..5120)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4478..4497)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5291..5879
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5780..5799
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6033..6050
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6167..6188
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6203..6208,6209..6226,6227..6233)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6241..6257
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6246..6268
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6265..6281
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6265..6281
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6300..6320
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6302..6320
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6348..6574
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6575..6755
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6802..6927
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7420..7653
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7838..7882
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8113..8323
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8135..8331
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8182..8317
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8363..8965
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8363..8382)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8859..8879
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9008..9125
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcatgccgcg ctctttcctc gtcaggaagc cctccgaccc
     1261 caatcggaag cctaactaca gcgagctgca ggactctaat ccagagttta ccttccagca
     1321 gccctacgac caggcccacc tgctggcagc catcccacct ccggagatcc tcaaccccac
     1381 cgcctcgctg ccaatgctca tctgggactc tgtcctggcg ccccaagccc agccaattgc
     1441 ctgggcctcc cttcggctcc aggagagtcc cagggtggca gagctgacct ccctgtcaga
     1501 tgaggacagt gggaaaggct cccagccccc cagcccaccc tcaccggctc cttcgtcctt
     1561 ctcctctact tcagtctctt ccttggaggc cgaggcctat gctgccttcc caggcttggg
     1621 ccaagtgccc aagcagctgg cccagctctc tgaggccaag gatctccagg ctcgaaaggc
     1681 cttcaactgc aaatactgca acaaggaata cctcagcctg ggtgccctca agatgcacat
     1741 ccgaagccac acgctgccct gcgtctgcgg aacctgcggg aaggccttct ctaggccctg
     1801 gctgctacaa ggccatgtcc ggacccacac tggcgagaag cccttctcct gtccccactg
     1861 cagccgtgcc ttcgctgacc gctccaacct gcgggcccac ctccagaccc actcagatgt
     1921 caagaagtac cagtgccagg cgtgtgctcg gaccttctcc cgaatgtccc tgctccacaa
     1981 gcaccaagag tccggctgct caggatgtcc ccgctgccca actttcttgt acaaagtggt
     2041 tgatatcggt aagcctatcc ctaaccctct cctcggtctc gattctacgt agtaatgaac
     2101 tagtccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga
     2161 ctgagtagtt ctcatactgt tcgaacgcgt taagtcgaca atcaacctct ggattacaaa
     2221 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     2281 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     2341 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     2401 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     2461 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     2521 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     2581 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     2641 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     2701 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     2761 cggatctccc tttgggccgc ctccccgcgt cgactttaag accaatgact tacaaggcag
     2821 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     2881 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     2941 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     3001 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     3061 gaccctttta gtcagtgtgg aaaatctcta gcagtacgta tagtagttca tgtcatctta
     3121 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
     3181 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     3241 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     3301 ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     3361 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     3421 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc
     3481 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
     3541 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     3601 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     3661 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     3721 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     3781 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     3841 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     3901 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     3961 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     4021 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     4081 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
     4141 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     4201 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     4261 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     4321 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     4381 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     4441 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     4501 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     4561 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     4621 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     4681 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     4741 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     4801 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     4861 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     4921 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     4981 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     5041 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     5101 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     5161 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     5221 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     5281 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     5341 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     5401 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     5461 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     5521 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     5581 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     5641 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     5701 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     5761 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     5821 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     5881 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     5941 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     6001 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     6061 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     6121 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     6181 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     6241 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
     6301 caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc
     6361 aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga
     6421 gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg
     6481 aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga
     6541 tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg
     6601 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     6661 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     6721 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
     6781 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
     6841 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
     6901 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
     6961 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
     7021 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
     7081 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     7141 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     7201 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     7261 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     7321 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     7381 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     7441 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     7501 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     7561 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     7621 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     7681 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     7741 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     7801 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     7861 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     7921 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     7981 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     8041 aaccccgagg ggacccatgc attgcatctc aattagtcag caaccaggtg tggaaagtcc
     8101 ccaggctccc cagcaggcag aagtatgcaa agcatgcgtc tcaattagtc agcaaccata
     8161 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     8221 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     8281 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctttct
     8341 agaggtacca ccggagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg
     8401 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
     8461 gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt
     8521 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgccgt
     8581 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
     8641 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
     8701 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
     8761 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
     8821 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
     8881 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
     8941 gacccgcaag cccggtgcca gttaacgaat tctagatctt gagacaaatg gcagtattca
     9001 tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
     9061 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
     9121 attttcgggt ttattacagg gacagcagag atccactttg gcgccggctc gaggcctgca
     9181 ggtgcaaaga tggataaagt tttaaacaga gaggaatctt tgcagctaat ggaccttcta
     9241 ggtcttgaaa ggagtgggaa tt
//
