LOCUS       sequence-222217-        9616 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive lentiviral expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9616)
  AUTHORS   Li J, Choi PS, Chaffer CL, Labella K, Hwang JH, Giacomelli AO, Kim 
            JW, Ilic N, Doench JG, Ly SH, Dai C, Hagel K, Hong AL, Gjoerup O, 
            Goel S, Ge JY, Root DE, Zhao JJ, Brooks AN, Weinberg RA, Hahn WC
  TITLE     An alternative splicing switch in FLNB promotes the mesenchymal cell
            state in human breast cancer.
  JOURNAL   Elife. 2018 Jul 30;7. pii: 37184. doi: 10.7554/eLife.37184.
  PUBMED    30059005
REFERENCE   2  (bases 1 to 9616)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-07-30"; volume: "7. pii: 37184. doi"; pages: " 
            10.7554/eLife.37184"
COMMENT     Sequence Label: pLX317-MBNL1
FEATURES             Location/Qualifiers
     source          1..9616
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2370..2394)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2402..2443
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2554..3142
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2607..2627)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3025..3036)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3214..3447
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3525..3646
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3562..3581)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3616..3635
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3686..3821
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3841..3860)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3842..3860)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3870..3887)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3870..3886)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3879..3901)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4028..4483
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4115..4134)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4325..4346
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4509..4613
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4614..4682,4683..5474)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4832..4851)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5645..6233
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6134..6153
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6387..6404
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6521..6542
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6557..6562,6563..6580,6581..6587)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6595..6611
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6600..6622
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6619..6635
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6619..6635
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6654..6674
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6656..6674
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6702..6928
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6929..7109
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7156..7281
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7774..8007
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8192..8236
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8467..8677
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8489..8685
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8536..8671
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8717..9319
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8717..8736)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9213..9233
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9362..9479
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcatggctgt tagtgtcaca ccaattcggg acacaaaatg
     1261 gctaacactg gaagtatgta gagagttcca gagggggact tgctcacggc cagacacgga
     1321 atgtaaattt gcacatcctt cgaaaagctg ccaagttgaa aatggacgag taatcgcctg
     1381 ctttgattca ttgaaaggcc gttgctccag ggagaactgc aaatatcttc atccaccccc
     1441 acatttaaaa acgcagttgg agataaatgg acgcaataac ttgattcagc agaagaacat
     1501 ggccatgttg gcccagcaaa tgcaactagc caatgccatg atgcctggtg ccccattaca
     1561 acccgtgcca atgttttcag ttgcaccaag cttagccacc aatgcatcag cagccgcctt
     1621 taatccctat ctgggacctg tttctccaag cctggtcccg gcagagatct tgccgactgc
     1681 accaatgttg gttacaggga atccgggtgt ccctgtacct gcagctgctg cagctgctgc
     1741 acagaaatta atgcgaacag acagacttga ggtatgtcga gagtaccaac gtggcaattg
     1801 caaccgagga gaaaatgatt gtcggtttgc tcatcctgct gacagcacaa tgattgacac
     1861 caatgacaac acagtcactg tgtgtatgga ttacatcaaa gggagatgct ctcgggaaaa
     1921 gtgcaaatac tttcatcccc ctgcacattt gcaagccaag atcaaggctg cccaatacca
     1981 ggtcaaccag gctgcagctg cacaggctgc agccaccgca gctgccatgg gaattcctca
     2041 agctgtactt cccccattac caaagaggcc tgctcttgaa aaaaccaacg gtgccaccgc
     2101 agtctttaac actggtattt tccaatacca acaggctcta gccaacatgc agttacaaca
     2161 gcatacagca tttctcccac caggctcaat attgtgcatg acacccgcta caagtgttgt
     2221 tcccatggtg cacggtgcta cgccagccac tgtgtccgca gcaacaacat ctgccacaag
     2281 tgttcccttc gctgcaacag ccacagccaa ccagataccc ataatatctg ccgaacatct
     2341 gactagccac aagtatgtta cccagatgta cccaactttc ttgtacaaag tggttgatat
     2401 cggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat gaactagtcc
     2461 gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg acgatgtgaa
     2521 ggtactcggc aacagaccac gcgttaagtc gacaatcaac ctctggatta caaaatttgt
     2581 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     2641 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     2701 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     2761 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     2821 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     2881 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     2941 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     3001 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     3061 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     3121 tccctttggg ccgcctcccc gcgtcgactt taagaccaat gacttacaag gcagctgtag
     3181 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     3241 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     3301 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     3361 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     3421 tttagtcagt gtggaaaatc tctagcagta cgtatagtag ttcatgtcat cttattattc
     3481 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     3541 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     3601 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     3661 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     3721 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     3781 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg
     3841 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
     3901 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     3961 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     4021 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     4081 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     4141 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     4201 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     4261 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     4321 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     4381 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     4441 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
     4501 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     4561 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     4621 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     4681 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     4741 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     4801 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     4861 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     4921 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     4981 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     5041 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     5101 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     5161 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     5221 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     5281 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     5341 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     5401 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     5461 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     5521 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     5581 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     5641 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     5701 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     5761 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     5821 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     5881 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     5941 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     6001 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     6061 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     6121 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     6181 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     6241 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     6301 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     6361 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     6421 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     6481 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     6541 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     6601 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
     6661 accctcacta aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact
     6721 cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa
     6781 gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca
     6841 acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt
     6901 atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg
     6961 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     7021 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     7081 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
     7141 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
     7201 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
     7261 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
     7321 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
     7381 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
     7441 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
     7501 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
     7561 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
     7621 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
     7681 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
     7741 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
     7801 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
     7861 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
     7921 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
     7981 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
     8041 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
     8101 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
     8161 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
     8221 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
     8281 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
     8341 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
     8401 gaggggaccc atgcattgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     8461 tccccagcag gcagaagtat gcaaagcatg cgtctcaatt agtcagcaac catagtcccg
     8521 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     8581 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     8641 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct ttctagaggt
     8701 accaccggag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     8761 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
     8821 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
     8881 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg ccgtggcggt
     8941 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
     9001 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
     9061 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
     9121 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
     9181 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
     9241 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
     9301 caagcccggt gccagttaac gaattctaga tcttgagaca aatggcagta ttcatccaca
     9361 attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     9421 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc
     9481 gggtttatta cagggacagc agagatccac tttggcgccg gctcgaggcc tgcaggtgca
     9541 aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct tctaggtctt
     9601 gaaaggagtg ggaatt
//
