LOCUS       sequence-222218-       10567 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ProB(I69E)A and codon optimized CarCBADE enzymes (in that order) in 
            pBbaA5k backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10567)
  AUTHORS   Shomar H, Gontier S, van den Broek NJF, Tejeda Mora H, Noga MJ, 
            Hagedoorn PL, Bokinsky G
  TITLE     Metabolic engineering of a carbapenem antibiotic synthesis pathway 
            in Escherichia coli.
  JOURNAL   Nat Chem Biol. 2018 Aug;14(8):794-800. doi: 
            10.1038/s41589-018-0084-6. Epub 2018 Jun 25.
  PUBMED    29942079
REFERENCE   2  (bases 1 to 10567)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-018-0084-6"; journalName: "Nat Chem Biol"; date: 
            "2018-08"; volume: "14"; issue: "8"; pages: "794-800"
COMMENT     Sequence Label: pCarCBADE_ProB(I69E)A
FEATURES             Location/Qualifiers
     source          1..10567
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(49..1131)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     1093..1112
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(1132..1209)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     1415..1434
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        join(1520..1525,1526..1543,1544..1550)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    1558..1574
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      8564..8635
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      8651..8678
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(8836..9424)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8916..8935)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(9512..9606)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(9637..10431)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9748..9767)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     10358..10377
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 gacgtcggtg cctaatgagt gagctaactt acattaattg cgttgcgctc actgcccgct
       61 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
      121 ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga cgggcaacag
      181 ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg
      241 ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataac atgagctgtc
      301 ttcggtatcg tcgtatccca ctaccgagat gtccgcacca acgcgcagcc cggactcggt
      361 aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg cagtgggaac
      421 gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc
      481 ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc agccagccag
      541 acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt gctggtgacc
      601 caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga aaataatact
      661 gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag tgcaggcagc
      721 ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc cactgacgcg
      781 ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc gttctaccat
      841 cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg
      901 cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg actgtttgcc
      961 cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg ccgcttccac
     1021 tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg aaacggtctg
     1081 ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca cattcaccac
     1141 cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt tgcgccattc
     1201 gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg aagcagccca
     1261 gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg
     1321 cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca
     1381 tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag
     1441 caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga
     1501 tcgtttaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggaattg
     1561 tgagcggata acaatttcag aattcaaaag atctaggagg aaaaaaatga gtgacagcca
     1621 gacgctggtg gtaaaactcg gcaccagtgt gctaacaggc ggatcgcgcc gtctgaaccg
     1681 tgcccatatc gttgaacttg ttcgccagtg cgcgcagtta catgccgccg ggcatcggat
     1741 tgttattgtg acgtcgggcg cgatcgccgc cggacgtgag cacctgggtt acccggaact
     1801 gccagcgact gaagcctcga aacaactgct ggcggcggta gggcagagtc gactgattca
     1861 actgtgggaa cagctgtttt cgatttatgg cattcacgtc gggcaaatgc tgctgacccg
     1921 tgctgatatg gaagaccgag aacgcttcct gaacgcccgc gacaccctgc gtgcgttgct
     1981 cgataacaat atcgttccgg taatcaatga gaacgatgct gtcgctacgg cagagattaa
     2041 ggtcggcgat aacgataacc tttctgcgct ggcggcgatt ctggcgggtg ccgataaact
     2101 gttgctgctg accgatcaaa aaggtttgta taccgctgac ccgcgcagca atccgcaggc
     2161 agaactgatt aaagatgttt acggcattga tgacgcactg cgcgcgattg ccggtgacag
     2221 cgtttcaggc ctcggaactg gcggcatgag taccaaattg caggccgctg acgtggcttg
     2281 ccgtgcgggt atcgacacca ttattgccgc gggcagcaag ccgggcgtta ttggtgatgt
     2341 gatggaaggc atttccgtcg gtacgctgtt ccatgcccag gcgactccgc ttgaaaaccg
     2401 taaacgctgg attttcggtg cgccgccggc gggtgaaatc acggtagatg aaggggcaac
     2461 tgccgccatt ctggaacgcg gcagctccct gttgccgaaa ggcattaaaa gcgtgactgg
     2521 caatttctcg cgtggtgaag tcatccgcat ttgcaacctc gaaggccgcg atatcgccca
     2581 cggcgtcagt cgttacaaca gcgatgcatt acgccgtatt gccggacacc actcgcaaga
     2641 aattgatgca atactgggat atgaatacgg cccggttgcc gttcaccgtg atgacatgat
     2701 tacccgttaa ggagcaggct gatgctggaa caaatgggca ttgccgcgaa gcaagcctcg
     2761 tataaattag cgcaactctc cagccgcgaa aaaaatcgcg tgctggaaaa aatcgccgat
     2821 gaactggaag cacaaagcga aatcatcctc aacgctaacg cccaggatgt tgctgacgcg
     2881 cgagccaatg gccttagcga agcgatgctt gaccgtctgg cactgacgcc cgcacggctg
     2941 aaaggcattg ccgacgatgt acgtcaggtg tgcaacctcg ccgatccggt ggggcaggta
     3001 atcgatggcg gcgtactgga cagcggcctg cgtcttgagc gtcgtcgcgt accgctgggg
     3061 gttattggcg tgatttatga agcgcgcccg aacgtgacgg ttgatgtcgc ttcgctgtgc
     3121 ctgaaaaccg gtaatgcggt gatcctgcgc ggtggcaaag aaacgtgtcg cactaacgct
     3181 gcaacggtgg cggtgattca ggacgccctg aaatcctgcg gcttaccggc gggtgccgtg
     3241 caggcgattg ataatcctga ccgtgcgctg gtcagtgaaa tgctgcgtat ggataaatac
     3301 atcgacatgc tgatcccgcg tggtggcgct ggtttgcata aactgtgccg cgaacagtcg
     3361 acgatcccgg tgatcacagg tggtataggc gtatgccata tttacgttga tgaaagtgca
     3421 gagatcgctg aagcattaaa agtgatcgtc aacgccaaaa ctcagcgtcc gagcacatgt
     3481 aatacggtag aaacgttgct ggtgaataaa aacatcgccg atagcttcct gcccgtatta
     3541 agcaaacaaa tggcggaaag cggcgtgaca ttacacgcag atgcagctgc gctgacacag
     3601 ttgcaggcag gccccgcgaa ggtggtggcg gttaaagccg aagagtatga cgatgagttt
     3661 ctgtcattag atttgaacgt caaaatcgtc agcgatcttg acgatgccat cgcccatatt
     3721 cgtgaacacg gcacgcaaca ctccgatgcg atcctgaccc gcgatatgcg caacgcccag
     3781 cgttttgtta acgaagtgga ttcgtccgct gtttacgtta acgcctctac gcgttttacc
     3841 gacggcggcc agtttggact gggtgcggaa gtggcggtaa gcacacaaaa actgcacgca
     3901 cgtggcccaa tggggctgga agcactgacc acttacaagt ggatcggcat tggtgattac
     3961 accattcgtg cgtaatagga ggaaaaaaat gtccgagatc gtgaaattta accctgtgat
     4021 ggcttctggc ttcggcgcat acattgatca ccgtgacttc ctggaagcta aaacggaaac
     4081 gatcaaaaac ctgctgatgc gccaaggttt cgtggttgtc aaaaacctgg atattgactc
     4141 cgatactttc cgtgatatct actctgcata cggtaccatc gtcgaatacg cggatgaaaa
     4201 aattggtgtt ggttttggtt atcgcgatac cctgaaactg gaaggcgaaa aaggtaaaat
     4261 cgttaccggt cgtggtcaac tgcctttcca cgctgacggt ggtctgctgc tgtctcaggt
     4321 cgatcaggtt ttcctgtacg cggcggagat taaaaacgtc aaattccgcg gtgcaactac
     4381 cgtttgcgat cacgctctgg cgtgccagga aatgccagca cacctgctgc gcgttctgga
     4441 agaggagacg ttcgaagttc gtgtgctgga acgtggttac tacgtggatg tgtcccctga
     4501 tggttggttc aaagtgccgg tgttcactga tctgggttgg gttcgtaaaa tgctgatcta
     4561 tttcccattc gatgaaggcc agccggcaag ctgggaaccg cgtattgttg gttttaccga
     4621 ccacgaaacc caggcattct ttcaagaact gggtgcgttc ctgaaacagc cgcgttatta
     4681 ctacaaacat ttctgggaag acggtgacct gctgattatg gataaccgtc gtgttatcca
     4741 cgagcgtgaa gaatttaacg atgatgacat cgttcgtcgt ctgtaccgcg gccaaactgc
     4801 agatatctaa aggaggaaaa aatggtattc gaagaaaact ccgatgaagt acgcgtgatt
     4861 accctggatc acccgaacaa acataaccca ttttcccgta ccctggaaac tagcgtgaag
     4921 gacgctctgg ctcgtgcaaa cgcggatgat agcgtgcgtg cggtcgttgt gtatggcggc
     4981 gctgaacgta gcttcagcgc aggcggtgat ttcaacgaag tcaagcagct gtctcgctcc
     5041 gaagatattg aagaatggat cgatcgtgtg atcgacctgt atcaggcagt cctgaacgta
     5101 aacaaaccga cgatcgcggc cgttgatggc tacgcgatcg gtatgggctt ccaattcgct
     5161 ctgatgtttg accagcgcct gatggccagc accgcgaact tcgtgatgcc ggaactgaaa
     5221 cacggtatcg gttgctctgt aggtgcagca atcctgggtt tcacccatgg tttctccacc
     5281 atgcaagaaa tcatctacca gtgccagtct ctggatgcac cgcgctgcgt cgactaccgc
     5341 ctggtgaatc aggtcgtaga gtcttccgcg ctgctggacg cggcgatcac tcaggcgcac
     5401 gtgatggcgt cctacccggc ttccgcattc attaacacga agcgtgcggt aaacaagccg
     5461 ttcattcacc tgctggaaca gacccgtgat gcttccaaag cagtgcacaa agccgcattc
     5521 caggcgcgtg atgcccaggg tcacttcaaa aacgttctgg gtaagaaata ctgaggatct
     5581 aggaggaaaa aaatgagcaa tagcttctgc gtcgtttaca aaggttccga tactgacatt
     5641 aacaacattc agcgtgattt cgacggtaaa ggcgaagccc tgtctaacgg ctatctgttc
     5701 atcgaacaga acggccacta ccagaaatgc gaaatggagc gtggcacggc gtacctgatt
     5761 ggttctctgt ataaccgtac tttcctgatt ggcctggcgg gcgtttggga aggtgaagca
     5821 tacctggcta acgatgctga actgctggcc ctgctgttta cgcgcctggg tgccaacgca
     5881 ctggctctgg cggaaggcga tttctgcttt ttcattgatg aaccgaatgg tgagctgacc
     5941 gttatcacgg aatctcgcgg cttctccccg gtccatgttg ttcaaggtaa gaaggcatgg
     6001 atgacgaaca gcctgaaact ggtaactgct gccgaaggcg agggcgcact gtggtttgaa
     6061 gaagaagctc tggtgtgcca gtctctgatg cgtgccgata cttatacgcc ggttaagaac
     6121 gcgcagcgtc tgaaaccggg cgcagtgcac gttctgaccc acgatagcga aggctacagc
     6181 tttgtagagt ctcgtaccct gaccactccg gcatccaacc aactgctggc tctgccgcgt
     6241 gaaccgctgc tggcactgat cgatcgctac ctgaacgctc cgctggagga cctggcacca
     6301 cgcttcgaca cggttggtat cccgctgagc ggtggtctgg actcttccct ggtgactgcc
     6361 ctggctagcc gccatttcaa gaaactgaac acgtattcca tcggtactga gctgtccaac
     6421 gagtttgagt tcagccagca ggttgcagat gctctgggta cgcaccacca gatgaaaatc
     6481 ctgtccgaaa cggaagttat taacggtatc atcgagtcca tttactacaa cgaaatcttc
     6541 gacggtctga gcgcggaaat ccagagcggt ctgttcaacg tgtaccgtca ggctcagggc
     6601 caagtctcct gcatgctgac tggctatggt tctgacctgc tgttcggcgg catcctgaaa
     6661 cctggcgctc agtatgataa tccgaaccag ctgctggcgg aacaagtgta ccgcacccgt
     6721 tggaccggcg aatttgcgac ccacggtgcg tcttgctacg gcatcgatat ccgccacccg
     6781 ttttggtccc acagcctgat tagcctgtgc cacgccctgc accctgatta caagattttc
     6841 gataatgaag ttaaaaacat cctgcgtgaa tacgctgata gcctgcagct gctgccaaag
     6901 gacatcgttt ggcgtaaaaa gatcggtatt cacgaaggtt cttctgttaa tcaggcgttc
     6961 gcaaacgtac tgggttctac ggttgacaat taccagacca aatctcgttt cacttatcgt
     7021 gtttaccagg cgttcctgcg cggtcgcctg tccatcactg acgttacccc gtcccagctg
     7081 aaggatctga tcaaaaagga ctgaggatct aggaggaaaa aaatgcctaa cgatctttac
     7141 gctatctaca atcgttacac gtcaaggacc ctgtttttta aatactgtgc gacagcgact
     7201 ctaacgcaca gactgacacg tcggctgtcg ctttttacgt tgaaaaagtg ccttgcccgt
     7261 ccacagggac gccttttctc tctggttaat agcatttatt ttggcggaga aacgctggaa
     7321 gaggttcaga gtacagcaac ttttctggca cgctctggca ttgcttgcgt actggactac
     7381 gccgtggaag gggaaaatga cgagacgcag ttcgataagg caatggaaaa tacgctgcgc
     7441 cttatcgaga tgtcgcagca aacggatagc ctgccttttg tcgtgattaa accgtcgtct
     7501 ctgggtagtg ttgctgtgta tgctcggcaa agtgaaaggc tcgcgctgga tgaagcatct
     7561 gcaagtgcat ggtcacgcat cgttacgcgt ttctcacgtc tgttcgatta tgcccgctct
     7621 catggcgtgc atgtgatggt tgatgcagag cagaccgcga ttcagcccgc agtggatcgt
     7681 ttggttctag acatgatgcg tgaatttaac cgcgatagcg cggtgatcac gctgacgctg
     7741 caattttatc taaaggatca attgcgtttt ctcgacgaat gctatcagcg agcctgtcag
     7801 gataatttcc tgttcggcgt gaaagtagtg cgcggtgctt atctggaaga agagaaacga
     7861 gtgaacggtg gcgtacgctg ttttgcaacc aaacaggaaa ccgatcgtag ttataacgcg
     7921 gcggtggatt acattgcact gcgtctggat cgaatcgcac cgtttttcgc cacgcacaac
     7981 gaggaaagcc tcgctttgat tatgtcgagt gagtcgctac gcgcggggcg cacctgggta
     8041 ggccagcttt acgggttggg cgatcatatt acttattcgc tgctgcaaac cggtttccgc
     8101 gtgtgcaaat acctgcctta cggtccgctc gataagtcgt tgccctattt actccgcagg
     8161 attgaagaga acgcggttgc ctcggcaacc ttcaaaaagg aaaataaact cttgcagaag
     8221 gagttgctgc gccgtctcgt aggagggatg taatgtcaaa atttcattgt catattcgcg
     8281 atacggctat cggtttcccc gtgtcggagg atgaaagcgt actcagttca gcctatgaag
     8341 ctggtgtcga actgccttat cgctgtgctt ctggttactg tggtgtatgc aaggtgcgcc
     8401 tgacgtccgg caacgtcaac atggatcatt ctggtgggat ctcacgtaaa gatatcgctg
     8461 atggttacat tctaccttgc tgttctgtgc cgctctctaa cctcgaaatt gaacctgtat
     8521 cgtcatgctg aggatccaaa ctcgagtaag gatctccagg catcaaataa aacgaaaggc
     8581 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
     8641 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatacctagg gcgttcggct
     8701 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     8761 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     8821 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     8881 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     8941 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     9001 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     9061 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     9121 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     9181 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     9241 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     9301 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     9361 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     9421 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     9481 ttaagggatt ttggtcatga ctagtgcttg gattctcacc aataaaaaac gcccggcggc
     9541 aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga tctatcaaca
     9601 ggagtccaag cgagctctcg aaccccagag tcccgctcag aagaactcgt caagaaggcg
     9661 atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc
     9721 agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata
     9781 gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac
     9841 catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcctcgc cgtcgggcat
     9901 gcgcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag
     9961 atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt
    10021 cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc
    10081 agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg
    10141 cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc
    10201 gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtcct gcagttcatt
    10261 cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg
    10321 gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct
    10381 ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca tgcgaaacga
    10441 tcctcatcct gtctcttgat cagatcatga tcccctgcgc catcagatcc ttggcggcaa
    10501 gaaagccatc cagtttactt tgcagggctt cccaacctta ccagagggcg ccccagctgg
    10561 caattcc
//
