LOCUS       sequence-22222-e         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   He W, Dorn DC, Erdjument-Bromage H, Tempst P, Moore MA, Massague J
  TITLE     Hematopoiesis controlled by distinct TIF1gamma and Smad4 branches of
            the TGFbeta pathway.
  JOURNAL   Cell. 2006 Jun 2. 125(5):929-41.
  PUBMED    16751102
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2006 
            Jun 2. 125(5):929-41."
COMMENT     Sequence Label: pRetroSuper Smad3
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(87..103)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(88..104)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             complement(122..538)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(138..154)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(170..192)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 tctgtatgag acacagatcc ccggacgagg tctgcgtgaa tttcaagaga attcacgcag
       61 acctcgtcct ttttggaaaa gcttatcgat accgtcgacc tcgagagatc gatctaattc
      121 cggcgcctag agaaggagtg agggctggat aaagggagga tcgaggcggg gtcgaacgag
      181 gaggttcaag ggggagagac ggggcggatg gaggaagagg aggcggaggc ttagggtgta
      241 caaagggctt gacccaggga ggggggtcaa aagccaaggc ttcccaggtc acgatgtagg
      301 ggacctggtc tgggtgtcca tgcgggccag gtgaaaagac cttgatctta acctgggtga
      361 tgaggtctcg gttaaaggtg ccgtctcgcg gccatccgac gttaaaggtt ggccattctg
      421 cagagcagaa ggtaacccaa cgtctcttct tgacatctac cgactggttg tgagcgatcc
      481 gctcgacatc tttccagtga cctaaggtca aacttaaggg agtggtaaca gtctggccct
      541 aattttcaga caaatacaga aacacagtca gacagagaca acacagaacg atgctgcagc
      601 gctgcagcag acaagacgcg cggcttcggt tccaaaccga aagcaaaaat tcagacggag
      661 gcgggaactg ttttaggttc tcgtctccta ccagaaccac atatcctgac ggggtcggat
      721 tccacatcga ctcccttcct caggtcgggc cacaaaaaac ggcccccaaa gtccctggga
      781 cgtctcccag ggttgcggcc gggtgttcag aactcgtcag ttccaccacg ggtccgccag
      841 atacagagct agttagctaa ctagtacaga cgcaggcgca aacattagat gccggcacaa
//
