LOCUS       sequence-222221-        9226 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive lentiviral expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9226)
  AUTHORS   Li J, Choi PS, Chaffer CL, Labella K, Hwang JH, Giacomelli AO, Kim 
            JW, Ilic N, Doench JG, Ly SH, Dai C, Hagel K, Hong AL, Gjoerup O, 
            Goel S, Ge JY, Root DE, Zhao JJ, Brooks AN, Weinberg RA, Hahn WC
  TITLE     An alternative splicing switch in FLNB promotes the mesenchymal cell
            state in human breast cancer.
  JOURNAL   Elife. 2018 Jul 30;7. pii: 37184. doi: 10.7554/eLife.37184.
  PUBMED    30059005
REFERENCE   2  (bases 1 to 9226)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-07-30"; volume: "7. pii: 37184. doi"; pages: " 
            10.7554/eLife.37184"
COMMENT     Sequence Label: pLX317-EGFP
FEATURES             Location/Qualifiers
     source          1..9226
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(1244..1246,1247..1249,1250..1960)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1289..1310)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1550..1569)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1897..1918
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(1980..2004)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2012..2053
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2164..2752
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2217..2237)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2635..2646)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2824..3057
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3135..3256
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3172..3191)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3226..3245
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3296..3431
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3451..3470)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3452..3470)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3480..3497)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3480..3496)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3489..3511)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3638..4093
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3725..3744)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3935..3956
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4119..4223
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4224..4292,4293..5084)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4442..4461)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5255..5843
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5744..5763
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5997..6014
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6131..6152
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6167..6172,6173..6190,6191..6197)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6205..6221
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6210..6232
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6229..6245
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6229..6245
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6264..6284
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6266..6284
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6312..6538
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6539..6719
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6766..6891
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7384..7617
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7802..7846
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8077..8287
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8099..8295
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8146..8281
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8327..8929
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8327..8346)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8823..8843
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8972..9089
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagcag gctccgcggc cgcccccttc accatggtga gcaagggcga
     1261 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     1321 caagttcagc gtgtctggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     1381 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     1441 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     1501 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     1561 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     1621 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     1681 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggcgaactt
     1741 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     1801 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     1861 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     1921 cgccgccggg atcactctcg gcatggacga gctgtacaag tccaagggtg ggcgcgccga
     1981 cccagctttc ttgtacaaag tggttgatat cggtaagcct atccctaacc ctctcctcgg
     2041 tctcgattct acgtagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct
     2101 ttatatatct tgtggaaagg acgaatcgat tttgtatttg gaggccctac gcgttaagtc
     2161 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     2221 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     2281 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     2341 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     2401 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     2461 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     2521 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     2581 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     2641 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     2701 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcgtcgactt
     2761 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     2821 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     2881 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     2941 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     3001 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     3061 cgtatagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     3121 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     3181 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     3241 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc
     3301 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     3361 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     3421 ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
     3481 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     3541 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     3601 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     3661 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     3721 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     3781 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     3841 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     3901 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     3961 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     4021 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     4081 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     4141 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     4201 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     4261 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     4321 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     4381 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     4441 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     4501 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     4561 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     4621 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     4681 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     4741 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     4801 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     4861 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     4921 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     4981 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     5041 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     5101 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     5161 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     5221 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     5281 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     5341 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     5401 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     5461 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     5521 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     5581 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     5641 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     5701 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     5761 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     5821 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     5881 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     5941 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     6001 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     6061 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     6121 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     6181 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     6241 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc
     6301 tgcaagctta atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag
     6361 ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag
     6421 gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac
     6481 cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg
     6541 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     6601 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     6661 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     6721 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
     6781 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
     6841 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
     6901 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
     6961 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
     7021 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
     7081 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
     7141 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
     7201 aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga
     7261 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     7321 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     7381 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     7441 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     7501 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     7561 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     7621 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     7681 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     7741 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     7801 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     7861 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     7921 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     7981 attatcgttt cagacccacc tcccaacccc gaggggaccc atgcattgca tctcaattag
     8041 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     8101 cgtctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     8161 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     8221 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     8281 ctaggctttt gcaaaaagct ttctagaggt accaccggag cttaccatga ccgagtacaa
     8341 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
     8401 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg
     8461 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     8521 ggtcgcggac gacggcgccg ccgtggcggt ctggaccacg ccggagagcg tcgaagcggg
     8581 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     8641 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     8701 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     8761 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
     8821 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     8881 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gccagttaac gaattctaga
     8941 tcttgagaca aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt
     9001 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
     9061 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccac
     9121 tttggcgccg gctcgaggcc tgcaggtgca aagatggata aagttttaaa cagagaggaa
     9181 tctttgcagc taatggacct tctaggtctt gaaaggagtg ggaatt
//
