LOCUS       sequence-222222-        8378 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of CD44 variant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8378)
  TITLE     Georg Weber Lab Plasmids
REFERENCE   2  (bases 1 to 8378)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pBabe-CD44v4
FEATURES             Location/Qualifiers
     source          1..8378
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    257..614
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             679..1095
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1025..1047
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1063..1079
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1105..1479
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1417..1433
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     misc_feature    join(3585..4159,4160..4162,4163..4171)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3752..3769)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3979..3998
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4172..4174,4175..4177,4178..4891)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4172..4174,4175..4177,4178..4888)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4217..4238)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4478..4497)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4825..4846
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5077..5546
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5676..5693)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5847..6435)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5927..5946)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(6559..6627,6628..7419)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6777..6796)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     LTR             join(8101..8378,1..192)
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 gcttctgctc cccgagctca ataaaagagc ccacaacccc tcactcggcg cgccagtcct
       61 ccgattgact gagtcgcccg ggtacccgtg tatccaataa accctcttgc agttgcatcc
      121 gacttgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact acccgtcagc
      181 gggggtcttt catttggggg ctcgtccggg atcgggagac ccctgcccag ggaccaccga
      241 cccaccaccg ggaggtaagc tggccagcaa cttatctgtg tctgtccgat tgtctagtgt
      301 ctatgactga ttttatgcgc ctgcgtcggt actagttagc taactagctc tgtatctggc
      361 ggacccgtgg tggaactgac gagttcggaa cacccggccg caaccctggg agacgtccca
      421 gggacttcgg gggccgtttt tgtggcccga cctgagtcca aaaatcccga tcgttttgga
      481 ctctttggtg cacccccctt agaggaggga tatgtggttc tggtaggaga cgagaaccta
      541 aaacagttcc cgcctccgtc tgaatttttg ctttcggttt gggaccgaag ccgcgccgcg
      601 cgtcttgtct gctgcagcat cgttctgtgt tgtctctgtc tgactgtgtt tctgtatttg
      661 tctgaaaata tgggcccggg ccagactgtt accactccct taagtttgac cttaggtcac
      721 tggaaagatg tcgagcggat cgctcacaac cagtcggtag atgtcaagaa gagacgttgg
      781 gttaccttct gctctgcaga atggccaacc tttaacgtcg gatggccgcg agacggcacc
      841 tttaaccgag acctcatcac ccaggttaag atcaaggtct tttcacctgg cccgcatgga
      901 cacccagacc aggtccccta catcgtgacc tgggaagcct tggcttttga cccccctccc
      961 tgggtcaagc cctttgtaca ccctaagcct ccgcctcctc ttcctccatc cgccccgtct
     1021 ctcccccttg aacctcctcg ttcgaccccg cctcgatcct ccctttatcc agccctcact
     1081 ccttctctag gcgcccccat atggccatat gagatcttat atggggcacc cccgcccctt
     1141 gtaaacttcc ctgaccctga catgacaaga gttactaaca gcccctctct ccaagctcac
     1201 ttacaggctc tctacttagt ccagcacgaa gtctggagac ctctggcggc agcctaccaa
     1261 gaacaactgg accgaccggt ggtacctcac ccttaccgag tcggcgacac agtgtgggtc
     1321 cgccgacacc agactaagaa cctagaacct cgctggaaag gaccttacac agtcctgctg
     1381 accaccccca ccgccctcaa agtagacggc atcgcagctt ggatacacgc cgcccacgtg
     1441 aaggctgccg accccggggg tggaccatcc tctagactgc cggatccatt taaattcgaa
     1501 ttcctgcagg cctcgagggc cggcgcgccg cggtggcggc cgctctagcc cctcgctcgc
     1561 ccacgccatg gacaagtttt ggtggcacac agcttgggga ctttgcctct tgcagttgag
     1621 cctggcacat cagcagatcg atttgaatgt aacctgccgc tacgcaggtg tattccatgt
     1681 ggagaaaaat ggccgctaca gtatctcccg gactgaggca gctgacctct gccaggcttt
     1741 caacagtacc ttacccacca tggaccaaat gaagttggcc ctgagcaagg gttttgaaac
     1801 atgcaggtat gggttcatag aaggaaatgt ggtaattccg aggattcatc ccaacgctat
     1861 ctgtgcagcc aaccacacag gagtatatat cctcgtcacg tccaacacct cccactatga
     1921 cacatattgc ttcaatgcct cagcccctcc tgaagaagac tgtacatcag tcacagacct
     1981 acccaattcc ttcgatggac ccgttaccat aactattgtc aaccgtgatg gtacacgcta
     2041 cagcaagaag ggcgagtata gaacacacca agaagacatc gatgcttcaa acattataga
     2101 tgacgatgtc agcagcggct ccaccatcga gaagagcacc ccagaaggct acattttgca
     2161 cacctacctt cctactgaac agcctactgg agatcaggat gactccttct ttatccggag
     2221 caccttggcc accattgcaa ctactccacg ggtttctgcc cgcacagaag acaaccagga
     2281 ctggacccag tggaagccaa accattcaaa cccggaagta ctacttcaga caaccaccag
     2341 gatggctgat atagacagaa tcagcaccag tgctcatgga gaaaattgga ccccggaacc
     2401 acagcctcct ttcaataacc atgagtatca ggatgaagag gagaccccac atgctacaag
     2461 cacaactcct aatagtacag cagaagcagc agctacccag caggagacgt ggtttcagaa
     2521 cggatggcag gggaagaacc cccctacccc aagcgaagac tcccatgtga cagaagggac
     2581 aactgcttcg gcccacaaca accatccaag tcaaggaata acaacacaga gtcaagagga
     2641 tgtttcctgg acagatttct tcgacccaat ctcacatcca atgggacaag gtcatcaaac
     2701 agaaagcaag gatacagact ccagtcatag tacaaccctt cagcctactg cagctccaaa
     2761 tacccatttg gtggaagact tgaacaggac aggaccactt tcagtgacaa ctccacagag
     2821 tcattctcag aacttctcta cattacatgg agagccggaa gaagacgaaa actatccaac
     2881 aacttctatt ctgccatcta gcactaagag cagcgctaaa gatgcaagaa gaggtggaag
     2941 tcttcccaca gatacaacta cttctgtaga aggctacacc tttcaatacc cagacacaat
     3001 ggaaaatggg actctcttcc cagtgacccc tgctaaaact gaggtctttg gagaaactga
     3061 agtgactctt gctactgact ccagcgttaa tgttgatggc tccttaccag gagatcgaga
     3121 ctcatccaag gactccaggg ggagttcccg cactgtgact catggatccg aattagctgg
     3181 acactcaagt gcgaaccagg acagtggagt gaccacaact tctggtccta tgaggagacc
     3241 tcagattcca gaatggctca tcatcttggc atctctcctg gcactggctc tgattcttgc
     3301 cgtctgcatc gcggtcaata gtaggagaag gtgtgggcag aagaaaaagc tggtgatcaa
     3361 cggtggcaat gggacagtgg aagacaggaa acccagtgag ctcaacgggg aggccagcaa
     3421 gtctcaggaa atggtgcatt tggtgaacaa ggaaccatca gagaccccag accagtgtat
     3481 gacagctgac gagacccgga atctgcagag tgtggacatg aagattgggg tttagtgcct
     3541 gggccgaacc aggggcggat cccccgtaaa ttccgccccc cccccccctc tccctccccc
     3601 ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg
     3661 ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc
     3721 ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg
     3781 aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg
     3841 accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca
     3901 cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata
     3961 gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc
     4021 cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt
     4081 gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt
     4141 tgaaaaacac gatgataata tggccacaac catggtgagc aagggcgagg agctgttcac
     4201 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     4261 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     4321 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
     4381 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     4441 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     4501 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     4561 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     4621 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     4681 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     4741 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
     4801 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     4861 cactctcggc atggacgagc tgtacaagta aagcgccagc acagtggtcg acgataaaat
     4921 aaaagatttt atttagtctc cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg
     4981 gcaagctagc ttaagtaacg ccattttgca aggcatggaa aaatacataa ctgagaatag
     5041 agaagttcag atcaaggtca ggaacagatg gaacagctga atatgggcca aacaggatat
     5101 ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggaaca gctgaatatg
     5161 ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg
     5221 gtccccagat gcggtccagc cctcagcagt ttctagagaa ccatcagatg tttccagggt
     5281 gccccaagga cctgaaatga ccctgtgcct tatttgaact aaccaatcag ttcgcttctc
     5341 gcttctgttc gcgcgcttct gctccccgag ctcaataaaa gagcccacaa cccctcactc
     5401 ggggcgccag tcctccgatt gactgagtcg cccgggtacc cgtgtatcca ataaaccctc
     5461 ttgcagttgc atccgacttg tggtctcgct gttccttggg agggtctcct ctgagtgatt
     5521 gactacccgt cagcgggggt ctttcacatg cagcatgtat caaaattaat ttggtttttt
     5581 ttcttaagta tttacattaa atggccatag ttgcattaat gaatcggcca acgcgcgggg
     5641 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     5701 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     5761 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     5821 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     5881 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     5941 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     6001 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     6061 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     6121 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     6181 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     6241 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     6301 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     6361 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     6421 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     6481 gaaaactcac gttaagggat tttggtcatg agacaataac cctgataaat gcttcaataa
     6541 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     6601 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     6661 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     6721 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     6781 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     6841 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     6901 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     6961 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     7021 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     7081 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     7141 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     7201 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     7261 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     7321 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     7381 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     7441 tatatacttt agattgattt gcggccggcc gcaaaaggat ctaggtgaag atcctttttg
     7501 ataatctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     7561 cgcgcacatt tccccgaaaa gtgccaccag ctttgctctt aggagtttcc taatacatcc
     7621 caaactcaaa tatataaagc atttgacttg ttctatgccc tagggggcgg ggggaagcta
     7681 agccagcttt ttttaacatt taaaatgtta attccatttt aaatgcacag atgtttttat
     7741 ttcataaggg tttcaatgtg catgaatgct gcaatattcc tgttaccaaa gctagtataa
     7801 ataaaaatag ataaacgtgg aaattactta gagtttctgt cattaacgtt tccttcctca
     7861 gttgacaaca taaatgcgct gctgagaagc cagtttgcat ctgtcaggat caatttccca
     7921 ttatgccagt catattaatt actagtcaat tagttgattt ttatttttga catatacatg
     7981 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
     8041 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca gatggaacag
     8101 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
     8161 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
     8221 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag
     8281 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
     8341 aactaaccaa tcagttcgct tctcgcttct gttcgcgc
//
