LOCUS       sequence-222228-       13149 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid encoding the nuclear localization of the FRET semi-synthetic
            biosensor measuring NAD+ concentration in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 13149)
  AUTHORS   Sallin O, Reymond L, Gondrand C, Raith F, Koch B, Johnsson K
  TITLE     Semisynthetic biosensors for mapping cellular concentrations of 
            nicotinamide adenine dinucleotides.
  JOURNAL   eLife. 2018 May 29;7. pii: 32638. doi: 10.7554/eLife.32638.
  PUBMED    29809136
REFERENCE   2  (bases 1 to 13149)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2018-05-29"; volume: "7. pii: 32638. doi"; pages: " 
            10.7554/eLife.32638"
COMMENT     Sequence Label: pEBTet_NAD-Snifit-nucl
FEATURES             Location/Qualifiers
     source          1..13149
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(770..788)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        856..960
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(961..1029,1030..1821)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1179..1198)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1992..2580
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2481..2500
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(2769..2789)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2787..3084
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3161..3760
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3161..3180)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3657..3677
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    3851..3985
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4123..4142)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        4419..4680
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     intron          4797..5369
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     5377..5396
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     5424..5443
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5424..5442
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5453..6073
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(5526..5545)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     polyA_signal    6115..6236
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        6291..6670
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6671..6874
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6825..6845
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    6876..6894
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6897..6915
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     6925..6949
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             7841..8725
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEISG"
     CDS             9407..9427
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9431..9451
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(9557..9574)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9563..9674
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 cctgctcctg cccctcctcc tgctcctgcc cctcctgctc ctgcccctcc cgctcctgct
       61 cctgctcctg ttccaccgtg ggtccctttg cagccaatgc aacttggacg tttttggggt
      121 ctccggacac catctctatg tcttggccct gatcctgagc cgcccggggc tcctggtctt
      181 ccgcctcctc gtcctcgtcc tcttccccgt cctcgtccat ggttatcacc ccctcttctt
      241 tgaggtccac tgccgccgga gccttctggt ccagatgtgt ctcccttctc tcctaggcca
      301 tttccaggtc ctgtacctgg cccctcgtca gacatgattc acactaaaag agatcaatag
      361 acatctttat tagacgacgc tcagtgaata cagggagtgc agactcctgc cccctccaac
      421 agccccccca ccctcatccc cttcatggtc gctgtcagac agatccaggt ctgaaaattc
      481 cccatcctcc gaaccatcct cgtcctcatc accaattact cgcagcccgg aaaactcccg
      541 ctgaacatcc tcaagatttg cgtcctgagc ctcaagccag gcctcaaatt cctcgtcccc
      601 ctttttgctg gacggtaggg atggggattc tcgggacccc tcctcttcct cttcaaggtc
      661 accagacaga gatgctactg gggcaacgga agaaaagctg ggtgcggcct gtgaggatca
      721 gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata
      781 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
      841 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
      901 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
      961 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     1021 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     1081 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     1141 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     1201 cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     1261 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     1321 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     1381 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     1441 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     1501 cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     1561 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     1621 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     1681 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     1741 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     1801 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     1861 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     1921 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     1981 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     2041 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     2101 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     2161 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     2221 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     2281 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     2341 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     2401 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     2461 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     2521 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     2581 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttg aagctgtccc
     2641 tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg
     2701 ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcga actagatgat
     2761 ccggctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     2821 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     2881 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     2941 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     3001 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     3061 gaagtagtga ggaggctttt ttggagggtg accgccacga ccggtgccgc caccatcccc
     3121 tgacccacgc ccctgacccc tcacaaggag acgaccttcc atgaccgagt acaagcccac
     3181 ggtgcgcctc gccacccgcg acgacgtccc ccgggccgta cgcaccctcg ccgccgcgtt
     3241 cgccgactac cccgccacgc gccacaccgt cgaccccgac cgccacatcg aacgcgtcac
     3301 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     3361 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     3421 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     3481 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     3541 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     3601 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     3661 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     3721 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga cgcccgcccc acgacccgca
     3781 gcgcccgacc gaaaggagcg cacgacccgg tccgacgcgg cccgacgggt ccgagggggg
     3841 tcgacctcga aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     3901 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     3961 caatgtatct tatcatgtct ggatctgatc cgaacccctt cctcgaccaa ttctcatgtt
     4021 tgacagctta tcatcgcaga tccgggcaac gttgttgcca ttgctcgacc aattctcatg
     4081 tttgacagct tatcatcgca gatccgtatg gtgcactctc agtacaatct gctctgatgc
     4141 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
     4201 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
     4261 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gtatctgagg
     4321 ggactagggt gtgtttaggc gaaaagcggg gcttcggttg tacgcggtta ggagtcccct
     4381 caggatatag tagtttcgct tttgcatagg gagggggaaa tgtagtctta tgcaatactc
     4441 ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag
     4501 caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa
     4561 cagacgggtc tgacatggat tggacgaacc actgaattcc gcattgcaga gatattgtat
     4621 ttaagtgcct agctcgatac aataaacgcc atttgaccat tcaccacatt ggtgtgcacc
     4681 tccaagctgg ccggggatcc tctagggcct ctgagctatt ccagaagtag tgaagaggct
     4741 tttttggagg cctaggcttt tgcaaaaagc tccggatcga tcctgagaac ttcagggtga
     4801 gtttggggac ccttgattgt tctttctttt tcgctattgt aaaattcatg ttatatggag
     4861 ggggcaaagt tttcagggtg ttgtttagaa tgggaagatg tcccttgtat caccatggac
     4921 cctcatgata attttgtttc tttcactttc tactctgttg acaaccattg tctcctctta
     4981 ttttcttttc attttctgta actttttcgt taaactttag cttgcatttg taacgaattt
     5041 ttaaattcac ttttgtttat ttgtcagatt gtaagtactt tctctaatca cttttttttc
     5101 aaggcaatca gggtatatta tattgtactt cagcacagtt ttagagaaca attgttataa
     5161 ttaaatgata aggtagaata tttctgcata taaattctgg ctggcgtgga aatattctta
     5221 ttggtagaaa caactacatc ctggtcatca tcctgccttt ctctttatgg ttacaatgat
     5281 atacactgtt tgagatgagg ataaaatact ctgagtccaa accgggcccc tctgctaacc
     5341 atgttcatgc cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt
     5401 ctcatcattt tggcaaagaa ttgtaatacg actcactata gggcgaattg atatgtctag
     5461 attagataaa agtaaagtga ttaacagcgc attagagctg cttaatgagg tcggaatcga
     5521 aggtttaaca acccgtaaac tcgcccagaa gctaggtgta gagcagccta cattgtattg
     5581 gcatgtaaaa aataagcggg ctttgctcga cgccttagcc attgagatgt tagataggca
     5641 ccatactcac ttttgccctt tagaagggga aagctggcaa gattttttac gtaataacgc
     5701 taaaagtttt agatgtgctt tactaagtca tcgcgatgga gcaaaagtac atttaggtac
     5761 acggcctaca gaaaaacagt atgaaactct cgaaaatcaa ttagcctttt tatgccaaca
     5821 aggtttttca ctagagaatg cattatatgc actcagcgct gtggggcatt ttactttagg
     5881 ttgcgtattg gaagatcaag agcatcaagt cgctaaagaa gaaagggaaa cacctactac
     5941 tgatagtatg ccgccattat tacgacaagc tatcgaatta tttgatcacc aaggtgcaga
     6001 gccagccttc ttattcggcc ttgaattgat catatgcgga ttagaaaaac aacttaaatg
     6061 tgaaagtggg tccgcgtaca gcggatcccg ggaattcaga tcttattaaa gcagaacttg
     6121 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     6181 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     6241 gtctggtcga atccatggcg cagaactggt aggtatggaa gatccgcgtt gacattgatt
     6301 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     6361 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     6421 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     6481 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     6541 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     6601 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     6661 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     6721 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
     6781 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     6841 gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata gagatctccc
     6901 tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg cctggagacg
     6961 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccggactct
     7021 agcgtttaaa cttaagctta ccaccatgga gggcgggctg gggcgtgctg tgtgcttgct
     7081 gaccggggcc tcccgcggct tcggccggac gctggccccg ctcctggcct cgctgctgtc
     7141 gcccggctcc gtgcttgtcc ttagcgactg gaacgacgag gcactgcgcc agctggaggc
     7201 cgagctgggc gccgagcggt ctggcctgcg cgtggtgcgg gtgcccgccg acctgggcgc
     7261 cgaggccggc ttgcagcagc tgctcggcgc cctgcgcgag ctcccccggc ccaaggggct
     7321 gcagcgactg ctgcttatca acaacgcggg ctctcttggg gatgtgtcca aaggcttcgt
     7381 ggacctgagt gactccactc aagtgaacaa ctactgggca ctgaacttga cctccatgct
     7441 ctgcctgact tccagcgtcc tgaaggcctt cccggacagt cctggcctca acagaaccgt
     7501 ggttaacatc tcgtccctct gtgccctgca acctttcaaa ggctgggcgc tgtactgtgc
     7561 aggaaaggct gctcgtgata tgctgttcca ggtcctggcg ctggaggaac ctaatgtgag
     7621 ggtgctgaac tatgccccag gtcctctgga cacagacatg cagcagttgg cccgggagac
     7681 ctccgtggac ccagacatgc gaaaagggct gcaggagctg aaggcaaagg ggaagctggt
     7741 ggattgcaag gtgtcagccc agaaactgct gagcttactg gaaaaggacg agttcaagtc
     7801 tggagcccac gtggacttct atgacaaaga attcggatcc gaaatcggta ctggctttcc
     7861 attcgacccc cattatgtgg aagtcctggg cgagcgcatg cactacgtcg atgttggtcc
     7921 gcgcgatggc acccctgtgc tgttcctgca cggtaacccg acctcctcct acgtgtggcg
     7981 caacatcatc ccgcatgttg caccgaccca tcgctgcatt gctccagacc tgatcggtat
     8041 gggcaaatcc gacaaaccag acctgggtta tttcttcgac gaccacgtcc gcttcatgga
     8101 tgccttcatc gaagccctgg gtctggaaga ggtcgtcctg gtcattcacg actggggctc
     8161 cgctctgggt ttccactggg ccaagcgcaa tccagagcgc gtcaaaggta ttgcatttat
     8221 ggagttcatc cgccctatcc cgacctggga cgaatggcca gaatttgccc gcgagacctt
     8281 ccaggccttc cgcaccaccg acgtcggccg caagctgatc atcgatcaga acgtttttat
     8341 cgagggtacg ctgccgatgg gtgtcgtccg cccgctgact gaagtcgaga tggaccatta
     8401 ccgcgagccg ttcctgaatc ctgttgaccg cgagccactg tggcgcttcc caaacgagct
     8461 gccaatcgcc ggtgagccag cgaacatcgt cgcgctggtc gaagaataca tggactggct
     8521 gcaccagtcc cctgtcccga agctgctgtt ctggggcacc ccaggcgttc tgatcccacc
     8581 ggccgaagcc gctcgcctgg ccaaaagcct gcctaactgc aaggctgtgg acatcggccc
     8641 gggtctgaat ctgctgcaag aagacaaccc ggacctgatc ggcagcgaga tcgcgcgctg
     8701 gctgtctact ctggagattt ccggtagcgg ccgccctcct cctccgccac cgccaccacc
     8761 gccgccgccg ccaccaccac ctcctccgcc tcctccacca ccaccgccac cgcctccacc
     8821 acctggcggc cgctctcggt ctctcgagat ggataaagac tgcgaaatga aacgcactac
     8881 cctggacagc ccgctgggta aactggaact gagcggttgc gagcagggcc tgcacgaaat
     8941 cattttcctg ggcaaaggta cgagcgctgc ggatgcggtc gaagtaccgg ctccggctgc
     9001 ggttctgggt ggcccggagc cgctgatgca ggctaccgca tggctgaacg catattttca
     9061 ccagccagag gcgattgaag agttccctgt accggccctg catcacccgg tatttcagca
     9121 ggaatccttt acccgtcagg tactgtggaa actgctgaaa gtagtgaaat tcggcgaggt
     9181 gatctcctat tcccatctgg cggcgctggc tggcaaccca gcggcaactg cggcggtgaa
     9241 aaccgctctg tctggtaatc cggtgccaat tctgatcccg tgccaccgtg tggttcaggg
     9301 cgacctggac gtaggtggct acgaaggcgg cctggctgtg aaagaatggc tgctggcgca
     9361 cgaaggtcac cgtctgggta aaccaggcct gggcggagct ccggatccaa aaaagaagag
     9421 aaaggtagat ccaaaaaaga agagaaaggt agatccaaaa aagaagagaa aggagctccg
     9481 ggcttctcct caataatgag ctagcggcgc gcccggccgc tcgagtctag agggcccgtt
     9541 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     9601 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     9661 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     9721 caggacagca agggggagga ttgggaagac aatagcaggc tagaggatcg atccccgccc
     9781 cggacgaact aaacctgact acgacatctc tgccccttct tcgcggggca gtgcatgtaa
     9841 tcccttcagt tggttggtac aacttgccaa ctgggccctg ttccacatgt gacacggggg
     9901 gggaccaaac acaaaggggt tctctgactg tagttgacat ccttataaat ggatgtgcac
     9961 atttgccaac actgagtggc tttcatcctg gagcagactt tgcagtctgt ggactgcaac
    10021 acaacattgc ctttatgtgt aactcttggc tgaagctctt acaccaatgc tgggggacat
    10081 gtacctccca ggggcccagg aagactacgg gaggctacac caacgtcaat cagaggggcc
    10141 tgtgtagcta ccgataagcg gaccctcaag agggcattag caatagtgtt tataaggccc
    10201 ccttgttaac cctaaacggg tagcatatgc ttcccgggta gtagtatata ctatccagac
    10261 taaccctaat tcaatagcat atgttaccca acgggaagca tatgctatcg aattagggtt
    10321 agtaaaaggg tcctaaggaa cagcgatatc tcccacccca tgagctgtca cggttttatt
    10381 tacatggggt caggattcca cgagggtagt gaaccatttt agtcacaagg gcagtggctg
    10441 aagatcaagg agcgggcagt gaactctcct gaatcttcgc ctgcttcttc attctccttc
    10501 gtttagctaa tagaataact gctgagttgt gaacagtaag gtgtatgtga ggtgctcgaa
    10561 aacaaggttt caggtgacgc ccccagaata aaatttggac ggggggttca gtggtggcat
    10621 tgtgctatga caccaatata accctcacaa accccttggg caataaatac tagtgtagga
    10681 atgaaacatt ctgaatatct ttaacaatag aaatccatgg ggtggggaca agccgtaaag
    10741 actggatgtc catctcacac gaatttatgg ctatgggcaa cacataatcc tagtgcaata
    10801 tgatactggg gttattaaga tgtgtcccag gcagggacca agacaggtga accatgttgt
    10861 tacactctat ttgtaacaag gggaaagaga gtggacgccg acagcagcgg actccactgg
    10921 ttgtctctaa cacccccgaa aattaaacgg ggctccacgc caatggggcc cataaacaaa
    10981 gacaagtggc cactcttttt tttgaaattg tggagtgggg gcacgcgtca gcccccacac
    11041 gccgccctgc ggttttggac tgtaaaataa gggtgtaata acttggctga ttgtaacccc
    11101 gctaaccact gcggtcaaac cacttgccca caaaaccact aatggcaccc cggggaatac
    11161 ctgcataagt aggtgggcgg gccaagatag gggcgcgatt gctgcgatct ggaggacaaa
    11221 ttacacacac ttgcgcctga gcgccaagca cagggttgtt ggtcctcata ttcacgaggt
    11281 cgctgagagc acggtgggct aatgttgcca tgggtagcat atactaccca aatatctgga
    11341 tagcatatgc tatcctaatc tatatctggg tagcataggc tatcctaatc tatatctggg
    11401 tagtatatgc tatcctaatc tgtatccggg tagcatatgc tatcctaata gagattaggg
    11461 tagtatatgc tatcctaatt tatatctggg tagcatatac tacccaaata tctggatagc
    11521 atatgctatc ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagc
    11581 ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt
    11641 atatgctatc ctaatttata tctgggtagc ataggctatc ctaatctata tctgggtagc
    11701 atatgctatc ctaatctata tctgggtagt atatgctatc ctaatctgta tccgggtagc
    11761 atatgctatc ctcatgcata tacagtcagc atatgatacc cagtagtaga gtgggagtgc
    11821 tatcctttgc atatgccgcc acctcccaag ggggcgtgaa ttttcgctgc ttgtcctttt
    11881 cctgctggtt gctcccattc ttaggtgaat ttaaggaggc caggctaaag ccgtcgcatg
    11941 tctgattgct caccaggtaa atgtcgctaa tgttttccaa cgcgagaagg tgttgagcgc
    12001 ggagctgagt gacgtgacaa catgggtatg cccaattgcc ccatgttggg aggacgaaaa
    12061 tggtgacaag acagatggcc agaaatacac caacagcacg catgatgtct actggggatt
    12121 tattctttag tgcgggggaa tacacggctt ttaatacgat tgagggcgtc tcctaacaag
    12181 ttacatcact cctgcccttc ctcaccctca tctccatcac ctccttcatc tccgtcatct
    12241 ccgtcatcac cctccgcggc agccccttcc accataggtg gaaaccaggg aggcaaatct
    12301 actccatcgt caaagctgca cacagtcacc ctgatattgc aggtaggagc gggctttgtc
    12361 ataacaaggt ccttaatcgc atccttcaaa acctcagcaa atatatgagt ttgtaaaaag
    12421 accatgaaat aacagacaat ggactccctt agcgggccag gttgtgggcc gggtccaggg
    12481 gccattccaa aggggagacg actcaatggt gtaagacgac attgtggaat agcaagggca
    12541 gttcctcgcc ttaggttgta aagggaggtc ttactacctc catatacgaa cacaccggcg
    12601 acccaagttc cttcgtcggt agtcctttct acgtgactcc tagccaggag agctcttaaa
    12661 ccttctgcaa tgttctcaaa tttcgggttg gaacctcctt gaccacgatg ctttccaaac
    12721 caccctcctt ttttgcgcct gcctccatca ccctgacccc ggggtccagt gcttgggcct
    12781 tctcctgggt catctgcggg gccctgctct atcgctcccg ggggcacgtc aggctcacca
    12841 tctgggccac cttcttggtg gtattcaaaa taatcggctt cccctacagg gtggaaaaat
    12901 ggccttctac ctggaggggg cctgcgcggt ggagacccgg atgatgatga ctgactactg
    12961 ggactcctgg gcctcttttc tccacgtcca cgacctctcc ccctggctct ttcacgactt
    13021 ccccccctgg ctctttcacg tcctctaccc cggcggcctc cactacctcc tcgaccccgg
    13081 cctccactac ctcctcgacc ccggcctcca ctgcctcctc gaccccggcc tccacctcct
    13141 gctcctgcc
//
