LOCUS       sequence-222229-        4670 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect cell expression vector for I-Ab alpha chain fused to BirA 
            biotinylation site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4670)
  AUTHORS   Moon JJ, Pepper M
  TITLE     Generation of Allergen-Specific Tetramers for a Murine Model of 
            Airway Inflammation.
  JOURNAL   Methods Mol Biol. 2018;1799:165-181. doi: 
            10.1007/978-1-4939-7896-0_14.
  PUBMED    29956152
REFERENCE   2  (bases 1 to 4670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2018"; volume: "1799:165-181. doi"; pages: " 
            10.1007/978-1-4939-7896-0_14"
COMMENT     Sequence Label: pR I-Ab alpha BirA
FEATURES             Location/Qualifiers
     source          1..4670
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        9..431
                     /label=MT promoter
                     /note="Drosophila metallothionein promoter"
     CDS             1183..1194
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             1237..1281
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     primer_bind     complement(2054..2071)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2054..2070)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2063..2085)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2279..2298)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2398..2420
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2458..2476)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2544..2648
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2649..2717,2718..3509)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3680..4268
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4169..4188
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4422..4439
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4556..4577
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4592..4597,4598..4615,4616..4622)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4630..4646
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4635..4657
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4654..4670
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4654..4670
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgattaccg ttgcaggaca ggatgtggtg cccgatgtga ctagctcttt gctgcaggcc
       61 gtcctatcct ctggttccga taagagaccc agaactccgg ccccccaccg cccaccgcca
      121 cccccataca tatgtggtac gcaagtaaga gtgcctgcgc atgccccatg tgccccacca
      181 agagctttgc atcccataca agtccccaaa gtggagaacc gaaccaattc ttcgcgggca
      241 gaacaaaagc ttctgcacac gtctccactc gaatttggag ccggccggcg tgtgcaaaag
      301 aggtgaatcg aacgaaagac ccgtgtgtaa agccgcgttt ccaaaatgta taaaaccgag
      361 agcatctggc caatgtgcat cagttgtggt cagcagcaaa atcaagtgaa tcatctcagt
      421 gcaactaaag gggaattcag gatgccgtgc agcagagctc tgattctggg ggtcctcgcc
      481 ctgaccacca tgctcagcct ctgtggaggt gaagacgaca ttgaggccga ccacgtaggc
      541 acctatggta taagtgtata tcagtctcct ggagacattg gccagtacac atttgaattt
      601 gatggtgatg agttgttcta tgtggacttg gataagaagg agactgtctg gatgcttcct
      661 gagtttggcc aattggcaag ctttgacccc caaggtggac tgcaaaacat agctgtagta
      721 aaacacaact tgggagtctt gactaagagg tcaaattcca ccccagctac caatgaggct
      781 cctcaagcga ctgtgttccc caagtcccct gtgctgctgg gtcagcccaa caccctcatc
      841 tgctttgtgg acaacatctt ccctcctgtg atcaacatca catggctcag aaatagcaag
      901 tcagtcgcag acggtgttta tgagaccagc ttcttcgtca accgtgacta ttccttccac
      961 aagctgtctt atctcacctt catcccttct gacgatgaca tttatgactg caaggtggaa
     1021 cactggggcc tggaggagcc ggttctgaaa cactgggaac ctgagattcc agcccccatg
     1081 tcagagctga cagagactgg aggtggagga tccactacag ctccatcagc tcagctcgaa
     1141 aaagagctcc aggccctgga gaaggaaaat gcacagctgg aatgggagtt gcaagcactg
     1201 gaaaaggaac tggctcaggg cggcagcggc ggcagcggcc tgaacgatat ttttgaagcg
     1261 cagaaaattg aatggcatga atgactcgac ctgcaggcat gcaagctatt cgatgcacac
     1321 tcacattctt ctcctaatac gataataaaa ctttccatga aaaatatgga aaaatatatg
     1381 aaaattgaga aatccaaaaa actgataaac gctctactta attaaaatag ataaatggga
     1441 gcggcaggaa tggcggagca tggccaagtt cctccgccaa tcagtcgtaa aacagaagtc
     1501 gtggaaagcg gatagaaaga atgttcgatt tgacgggcaa gcatgtctgc tatgtggcgg
     1561 attgcggagg aattgcactg gagaccagca aggttctcat gaccaagaat atagcggtga
     1621 gtgagcggga agctcggttt ctgtccagat cgaactcaaa acttgtccag ccagtcgctg
     1681 tcgaaactaa ttaagttaat gagtttttca tgttagtttc gcgctgagca acaattaagt
     1741 ttatgtttca gttcggctta gatttcgctg aaggacttgc cactttcaat caatacttta
     1801 gaacaaaatc aaaactcatt ctaatagctt ggtgttcatc ttttttttta atgataagca
     1861 ttttgtcgtt tatacttttt atatttcgat attaaaccac ctatgaagtc tattttaatc
     1921 gccagataag caatatattg tgtaaatatt tgtattcttt atcaggaaat tcagggagac
     1981 ggggaagtta ctatctacta aaagccaaac aatttcttac agttttactc tctctactct
     2041 agagtagctt ggcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     2101 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     2161 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc
     2221 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta
     2281 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
     2341 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     2401 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
     2461 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     2521 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     2581 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     2641 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     2701 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     2761 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     2821 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     2881 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     2941 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     3001 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     3061 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     3121 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     3181 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     3241 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     3301 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     3361 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     3421 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     3481 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     3541 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     3601 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     3661 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     3721 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     3781 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     3841 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     3901 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     3961 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     4021 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     4081 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     4141 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     4201 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     4261 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     4321 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     4381 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     4441 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     4501 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     4561 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     4621 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
//
