LOCUS       sequence-22223-e         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  AUTHORS   He W, Dorn DC, Erdjument-Bromage H, Tempst P, Moore MA, Massague J
  TITLE     Hematopoiesis controlled by distinct TIF1gamma and Smad4 branches of
            the TGFbeta pathway.
  JOURNAL   Cell. 2006 Jun 2. 125(5):929-41.
  PUBMED    16751102
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2006 
            Jun 2. 125(5):929-41."
COMMENT     Sequence Label: pRetroSuper Smad4
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(86..102)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(87..103)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             complement(121..537)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(137..153)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(169..191)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 tctgtatgag acacagatcc ccggtgtgca gttggaatgt attcaagaga tacattccaa
       61 ctgcacacct ttttggaaag cttatcgata ccgtcgacct cgagagatcg atctaattcc
      121 ggcgcctaga gaaggagtga gggctggata aagggaggat cgaggcgggg tcgaacgagg
      181 aggttcaagg gggagagacg gggcggatgg aggaagagga ggcggaggct tagggtgtac
      241 aaagggcttg acccagggag gggggtcaaa agccaaggct tcccaggtca cgatgtaggg
      301 gacctggtct gggtgtccat gcgggccagg tgaaaagacc ttgatcttaa cctgggtgat
      361 gaggtctcgg ttaaaggtgc cgtctcgcgg ccatccgacg ttaaaggttg gccattctgc
      421 agagcagaag gtaacccaac gtctcttctt gacatctacc gactggttgt gagcgatccg
      481 ctcgacatct ttccagtgac ctaaggtcaa acttaaggga gtggtaacag tctggcccta
      541 attttcagac aaatacagaa acacagtcag acagagacaa cacagaacga tgctgcagcg
      601 ctgcagcaga caagacgcgc ggcttcggtt ccaaaccgaa agcaaaaatt cagacggagg
      661 cgggaactgt tttaggttct cgtctcctac cagaaccaca tatcctgacg gggtcggatt
      721 ccacatcgac tcccttcctc aggtcgggcc acaaaaacgg cccccaaagt ccctggggac
      781 gtctcccagg gttgcggccg ggtgttcaga actcgtcagt tccaccacgg gtccgccaga
      841 tacagagcta gttagctaac tagtacagac gcaggcgcaa acattagatg ccggcacaaa
      901 cccgccca
//
