LOCUS       sequence-222240-       13118 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid encoding the nuclear localization of the FRET semi-synthetic
            biosensor measuring the ratio NADPH/NADP+ in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 13118)
  AUTHORS   Sallin O, Reymond L, Gondrand C, Raith F, Koch B, Johnsson K
  TITLE     Semisynthetic biosensors for mapping cellular concentrations of 
            nicotinamide adenine dinucleotides.
  JOURNAL   eLife. 2018 May 29;7. pii: 32638. doi: 10.7554/eLife.32638.
  PUBMED    29809136
REFERENCE   2  (bases 1 to 13118)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2018-05-29"; volume: "7. pii: 32638. doi"; pages: " 
            10.7554/eLife.32638"
COMMENT     Sequence Label: pEBTet_NADP-Snifit-nucl
FEATURES             Location/Qualifiers
     source          1..13118
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(757..775)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        843..947
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(948..1016,1017..1808)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1166..1185)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1979..2567
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2468..2487
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(2756..2776)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2774..3071
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3148..3747
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3148..3167)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3644..3664
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    3838..3972
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4110..4129)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        4406..4667
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     intron          4784..5356
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     5364..5383
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     5411..5430
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5411..5429
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5440..6060
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(5513..5532)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     polyA_signal    6102..6223
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        6278..6657
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6658..6861
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6812..6832
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    6863..6881
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6884..6902
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     6912..6936
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             7828..8712
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEISG"
     CDS             9394..9414
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9418..9438
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(9544..9561)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9550..9661
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ctcctcctgc tcctgcccct cctgctcctg cccctcccgc tcctgctcct gctcctgttc
       61 caccgtgggt ccctttgcag ccaatgcaac ttggacgttt ttggggtctc cggacaccat
      121 ctctatgtct tggccctgat cctgagccgc ccggggctcc tggtcttccg cctcctcgtc
      181 ctcgtcctct tccccgtcct cgtccatggt tatcaccccc tcttctttga ggtccactgc
      241 cgccggagcc ttctggtcca gatgtgtctc ccttctctcc taggccattt ccaggtcctg
      301 tacctggccc ctcgtcagac atgattcaca ctaaaagaga tcaatagaca tctttattag
      361 acgacgctca gtgaatacag ggagtgcaga ctcctgcccc ctccaacagc ccccccaccc
      421 tcatcccctt catggtcgct gtcagacaga tccaggtctg aaaattcccc atcctccgaa
      481 ccatcctcgt cctcatcacc aattactcgc agcccggaaa actcccgctg aacatcctca
      541 agatttgcgt cctgagcctc aagccaggcc tcaaattcct cgtccccctt tttgctggac
      601 ggtagggatg gggattctcg ggacccctcc tcttcctctt caaggtcacc agacagagat
      661 gctactgggg caacggaaga aaagctgggt gcggcctgtg aggatcagct tatcgatgat
      721 aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat
      781 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
      841 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
      901 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
      961 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     1021 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     1081 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     1141 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg
     1201 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     1261 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     1321 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     1381 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     1441 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg
     1501 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     1561 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     1621 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     1681 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     1741 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     1801 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     1861 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     1921 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     1981 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     2041 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     2101 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     2161 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     2221 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     2281 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     2341 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     2401 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     2461 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     2521 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     2581 cggccttttt acggttcctg gccttttgct ggccttgaag ctgtccctga tggtcgtcat
     2641 ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa
     2701 gaatcataat ggggaaggcc atccagcctc gcgtcgaact agatgatccg gctgtggaat
     2761 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     2821 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     2881 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     2941 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     3001 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     3061 ggcttttttg gagggtgacc gccacgaccg gtgccgccac catcccctga cccacgcccc
     3121 tgacccctca caaggagacg accttccatg accgagtaca agcccacggt gcgcctcgcc
     3181 acccgcgacg acgtcccccg ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     3241 gccacgcgcc acaccgtcga ccccgaccgc cacatcgaac gcgtcaccga gctgcaagaa
     3301 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     3361 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     3421 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     3481 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     3541 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     3601 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
     3661 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     3721 tgcatgaccc gcaagcccgg tgcctgacgc ccgccccacg acccgcagcg cccgaccgaa
     3781 aggagcgcac gacccggtcc gacgcggccc gacgggtccg aggggggtcg acctcgaaac
     3841 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     3901 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     3961 catgtctgga tctgatccga accccttcct cgaccaattc tcatgtttga cagcttatca
     4021 tcgcagatcc gggcaacgtt gttgccattg ctcgaccaat tctcatgttt gacagcttat
     4081 catcgcagat ccgtatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     4141 cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa
     4201 gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt
     4261 tttgcgctgc ttcgcgatgt acgggccaga tatacgcgta tctgagggga ctagggtgtg
     4321 tttaggcgaa aagcggggct tcggttgtac gcggttagga gtcccctcag gatatagtag
     4381 tttcgctttt gcatagggag ggggaaatgt agtcttatgc aatactcttg tagtcttgca
     4441 acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc
     4501 gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga
     4561 catggattgg acgaaccact gaattccgca ttgcagagat attgtattta agtgcctagc
     4621 tcgatacaat aaacgccatt tgaccattca ccacattggt gtgcacctcc aagctggccg
     4681 gggatcctct agggcctctg agctattcca gaagtagtga agaggctttt ttggaggcct
     4741 aggcttttgc aaaaagctcc ggatcgatcc tgagaacttc agggtgagtt tggggaccct
     4801 tgattgttct ttctttttcg ctattgtaaa attcatgtta tatggagggg gcaaagtttt
     4861 cagggtgttg tttagaatgg gaagatgtcc cttgtatcac catggaccct catgataatt
     4921 ttgtttcttt cactttctac tctgttgaca accattgtct cctcttattt tcttttcatt
     4981 ttctgtaact ttttcgttaa actttagctt gcatttgtaa cgaattttta aattcacttt
     5041 tgtttatttg tcagattgta agtactttct ctaatcactt ttttttcaag gcaatcaggg
     5101 tatattatat tgtacttcag cacagtttta gagaacaatt gttataatta aatgataagg
     5161 tagaatattt ctgcatataa attctggctg gcgtggaaat attcttattg gtagaaacaa
     5221 ctacatcctg gtcatcatcc tgcctttctc tttatggtta caatgatata cactgtttga
     5281 gatgaggata aaatactctg agtccaaacc gggcccctct gctaaccatg ttcatgcctt
     5341 cttctttttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg
     5401 caaagaattg taatacgact cactataggg cgaattgata tgtctagatt agataaaagt
     5461 aaagtgatta acagcgcatt agagctgctt aatgaggtcg gaatcgaagg tttaacaacc
     5521 cgtaaactcg cccagaagct aggtgtagag cagcctacat tgtattggca tgtaaaaaat
     5581 aagcgggctt tgctcgacgc cttagccatt gagatgttag ataggcacca tactcacttt
     5641 tgccctttag aaggggaaag ctggcaagat tttttacgta ataacgctaa aagttttaga
     5701 tgtgctttac taagtcatcg cgatggagca aaagtacatt taggtacacg gcctacagaa
     5761 aaacagtatg aaactctcga aaatcaatta gcctttttat gccaacaagg tttttcacta
     5821 gagaatgcat tatatgcact cagcgctgtg gggcatttta ctttaggttg cgtattggaa
     5881 gatcaagagc atcaagtcgc taaagaagaa agggaaacac ctactactga tagtatgccg
     5941 ccattattac gacaagctat cgaattattt gatcaccaag gtgcagagcc agccttctta
     6001 ttcggccttg aattgatcat atgcggatta gaaaaacaac ttaaatgtga aagtgggtcc
     6061 gcgtacagcg gatcccggga attcagatct tattaaagca gaacttgttt attgcagctt
     6121 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     6181 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggtcgaatc
     6241 catggcgcag aactggtagg tatggaagat ccgcgttgac attgattatt gactagttat
     6301 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
     6361 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     6421 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     6481 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     6541 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     6601 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
     6661 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
     6721 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
     6781 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
     6841 gaggtctata taagcagagc tctccctatc agtgatagag atctccctat cagtgataga
     6901 gatcgtcgac gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt
     6961 tttgacctcc atagaagaca ccgggaccga tccagcctcc ggactctagc gtttaaactt
     7021 aagcttacca ccatggaggg cgggctgggg cgtgctgtgt gcttgctgac cggggcctcc
     7081 cgcggcttcg gccggacgct ggccccgctc ctggcctcgc tgctgtcgcc cggctccgtg
     7141 cttgtcctta gcgcccgcaa cgacgaggca ctgcgccagc tggaggccga gctgggcgcc
     7201 gagcggtctg gcctgcgcgt ggtgcgggtg cccgccgacc tgggcgccga ggccggcttg
     7261 cagcagctgc tcggcgccct gcgcgagctc ccccggccca aggggctgca gcgactgctg
     7321 cttatcaaca acgcgggctc tcttggggat gtgtccaaag gcttcgtgga cctgagtgac
     7381 tccactcaag tgaacaacta ctgggcactg aacttgacct ccatgctctg cctgacttcc
     7441 agcgtcctga aggccttccc ggacagtcct ggcctcaaca gaaccgtggt taacatctcg
     7501 tccctctgtg ccctgcaacc tttcaaaggc tgggcgctgt actgtgcagg aaaggctgct
     7561 cgtgatatgc tgttccaggt cctggcgctg gaggaaccta atgtgagggt gctgaactat
     7621 gccccaggtc ctctggacac agacatgcag cagttggccc gggagacctc cgtggaccca
     7681 gacatgcgaa aagggctgca ggagctgaag gcaaagggga agctggtgga ttgcaaggtg
     7741 tcagcccaga aactgctgag cttactggaa aaggacgagt tcaagtctgg agcccacgtg
     7801 gacttctatg acaaagaatt cggatccgaa atcggtactg gctttccatt cgacccccat
     7861 tatgtggaag tcctgggcga gcgcatgcac tacgtcgatg ttggtccgcg cgatggcacc
     7921 cctgtgctgt tcctgcacgg taacccgacc tcctcctacg tgtggcgcaa catcatcccg
     7981 catgttgcac cgacccatcg ctgcattgct ccagacctga tcggtatggg caaatccgac
     8041 aaaccagacc tgggttattt cttcgacgac cacgtccgct tcatggatgc cttcatcgaa
     8101 gccctgggtc tggaagaggt cgtcctggtc attcacgact ggggctccgc tctgggtttc
     8161 cactgggcca agcgcaatcc agagcgcgtc aaaggtattg catttatgga gttcatccgc
     8221 cctatcccga cctgggacga atggccagaa tttgcccgcg agaccttcca ggccttccgc
     8281 accaccgacg tcggccgcaa gctgatcatc gatcagaacg tttttatcga gggtacgctg
     8341 ccgatgggtg tcgtccgccc gctgactgaa gtcgagatgg accattaccg cgagccgttc
     8401 ctgaatcctg ttgaccgcga gccactgtgg cgcttcccaa acgagctgcc aatcgccggt
     8461 gagccagcga acatcgtcgc gctggtcgaa gaatacatgg actggctgca ccagtcccct
     8521 gtcccgaagc tgctgttctg gggcacccca ggcgttctga tcccaccggc cgaagccgct
     8581 cgcctggcca aaagcctgcc taactgcaag gctgtggaca tcggcccggg tctgaatctg
     8641 ctgcaagaag acaacccgga cctgatcggc agcgagatcg cgcgctggct gtctactctg
     8701 gagatttccg gtagcggccg ccctcctcct ccgccaccgc caccaccgcc gccgccgcca
     8761 ccaccacctc ctccgcctcc tccaccacca ccgccaccgc ctccaccacc tggcggccgc
     8821 tctcggtctc tcgagatgga taaagactgc gaaatgaaac gcactaccct ggacagcccg
     8881 ctgggtaaac tggaactgag cggttgcgag cagggcctgc acgaaatcat tttcctgggc
     8941 aaaggtacga gcgctgcgga tgcggtcgaa gtaccggctc cggctgcggt tctgggtggc
     9001 ccggagccgc tgatgcaggc taccgcatgg ctgaacgcat attttcacca gccagaggcg
     9061 attgaagagt tccctgtacc ggccctgcat cacccggtat ttcagcagga atcctttacc
     9121 cgtcaggtac tgtggaaact gctgaaagta gtgaaattcg gcgaggtgat ctcctattcc
     9181 catctggcgg cgctggctgg caacccagcg gcaactgcgg cggtgaaaac cgctctgtct
     9241 ggtaatccgg tgccaattct gatcccgtgc caccgtgtgg ttcagggcga cctggacgta
     9301 ggtggctacg aaggcggcct ggctgtgaaa gaatggctgc tggcgcacga aggtcaccgt
     9361 ctgggtaaac caggcctggg cggagctccg gatccaaaaa agaagagaaa ggtagatcca
     9421 aaaaagaaga gaaaggtaga tccaaaaaag aagagaaagg agctccgggc ttctcctcaa
     9481 taatgagcta gcggcgcgcc cggccgctcg agtctagagg gcccgtttaa acccgctgat
     9541 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     9601 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     9661 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     9721 gggaggattg ggaagacaat agcaggctag aggatcgatc cccgccccgg acgaactaaa
     9781 cctgactacg acatctctgc cccttcttcg cggggcagtg catgtaatcc cttcagttgg
     9841 ttggtacaac ttgccaactg ggccctgttc cacatgtgac acgggggggg accaaacaca
     9901 aaggggttct ctgactgtag ttgacatcct tataaatgga tgtgcacatt tgccaacact
     9961 gagtggcttt catcctggag cagactttgc agtctgtgga ctgcaacaca acattgcctt
    10021 tatgtgtaac tcttggctga agctcttaca ccaatgctgg gggacatgta cctcccaggg
    10081 gcccaggaag actacgggag gctacaccaa cgtcaatcag aggggcctgt gtagctaccg
    10141 ataagcggac cctcaagagg gcattagcaa tagtgtttat aaggccccct tgttaaccct
    10201 aaacgggtag catatgcttc ccgggtagta gtatatacta tccagactaa ccctaattca
    10261 atagcatatg ttacccaacg ggaagcatat gctatcgaat tagggttagt aaaagggtcc
    10321 taaggaacag cgatatctcc caccccatga gctgtcacgg ttttatttac atggggtcag
    10381 gattccacga gggtagtgaa ccattttagt cacaagggca gtggctgaag atcaaggagc
    10441 gggcagtgaa ctctcctgaa tcttcgcctg cttcttcatt ctccttcgtt tagctaatag
    10501 aataactgct gagttgtgaa cagtaaggtg tatgtgaggt gctcgaaaac aaggtttcag
    10561 gtgacgcccc cagaataaaa tttggacggg gggttcagtg gtggcattgt gctatgacac
    10621 caatataacc ctcacaaacc ccttgggcaa taaatactag tgtaggaatg aaacattctg
    10681 aatatcttta acaatagaaa tccatggggt ggggacaagc cgtaaagact ggatgtccat
    10741 ctcacacgaa tttatggcta tgggcaacac ataatcctag tgcaatatga tactggggtt
    10801 attaagatgt gtcccaggca gggaccaaga caggtgaacc atgttgttac actctatttg
    10861 taacaagggg aaagagagtg gacgccgaca gcagcggact ccactggttg tctctaacac
    10921 ccccgaaaat taaacggggc tccacgccaa tggggcccat aaacaaagac aagtggccac
    10981 tctttttttt gaaattgtgg agtgggggca cgcgtcagcc cccacacgcc gccctgcggt
    11041 tttggactgt aaaataaggg tgtaataact tggctgattg taaccccgct aaccactgcg
    11101 gtcaaaccac ttgcccacaa aaccactaat ggcaccccgg ggaatacctg cataagtagg
    11161 tgggcgggcc aagatagggg cgcgattgct gcgatctgga ggacaaatta cacacacttg
    11221 cgcctgagcg ccaagcacag ggttgttggt cctcatattc acgaggtcgc tgagagcacg
    11281 gtgggctaat gttgccatgg gtagcatata ctacccaaat atctggatag catatgctat
    11341 cctaatctat atctgggtag cataggctat cctaatctat atctgggtag tatatgctat
    11401 cctaatctgt atccgggtag catatgctat cctaatagag attagggtag tatatgctat
    11461 cctaatttat atctgggtag catatactac ccaaatatct ggatagcata tgctatccta
    11521 atctatatct gggtagcata tgctatccta atctatatct gggtagcata ggctatccta
    11581 atctatatct gggtagcata tgctatccta atctatatct gggtagtata tgctatccta
    11641 atttatatct gggtagcata ggctatccta atctatatct gggtagcata tgctatccta
    11701 atctatatct gggtagtata tgctatccta atctgtatcc gggtagcata tgctatcctc
    11761 atgcatatac agtcagcata tgatacccag tagtagagtg ggagtgctat cctttgcata
    11821 tgccgccacc tcccaagggg gcgtgaattt tcgctgcttg tccttttcct gctggttgct
    11881 cccattctta ggtgaattta aggaggccag gctaaagccg tcgcatgtct gattgctcac
    11941 caggtaaatg tcgctaatgt tttccaacgc gagaaggtgt tgagcgcgga gctgagtgac
    12001 gtgacaacat gggtatgccc aattgcccca tgttgggagg acgaaaatgg tgacaagaca
    12061 gatggccaga aatacaccaa cagcacgcat gatgtctact ggggatttat tctttagtgc
    12121 gggggaatac acggctttta atacgattga gggcgtctcc taacaagtta catcactcct
    12181 gcccttcctc accctcatct ccatcacctc cttcatctcc gtcatctccg tcatcaccct
    12241 ccgcggcagc cccttccacc ataggtggaa accagggagg caaatctact ccatcgtcaa
    12301 agctgcacac agtcaccctg atattgcagg taggagcggg ctttgtcata acaaggtcct
    12361 taatcgcatc cttcaaaacc tcagcaaata tatgagtttg taaaaagacc atgaaataac
    12421 agacaatgga ctcccttagc gggccaggtt gtgggccggg tccaggggcc attccaaagg
    12481 ggagacgact caatggtgta agacgacatt gtggaatagc aagggcagtt cctcgcctta
    12541 ggttgtaaag ggaggtctta ctacctccat atacgaacac accggcgacc caagttcctt
    12601 cgtcggtagt cctttctacg tgactcctag ccaggagagc tcttaaacct tctgcaatgt
    12661 tctcaaattt cgggttggaa cctccttgac cacgatgctt tccaaaccac cctccttttt
    12721 tgcgcctgcc tccatcaccc tgaccccggg gtccagtgct tgggccttct cctgggtcat
    12781 ctgcggggcc ctgctctatc gctcccgggg gcacgtcagg ctcaccatct gggccacctt
    12841 cttggtggta ttcaaaataa tcggcttccc ctacagggtg gaaaaatggc cttctacctg
    12901 gagggggcct gcgcggtgga gacccggatg atgatgactg actactggga ctcctgggcc
    12961 tcttttctcc acgtccacga cctctccccc tggctctttc acgacttccc cccctggctc
    13021 tttcacgtcc tctaccccgg cggcctccac tacctcctcg accccggcct ccactacctc
    13081 ctcgaccccg gcctccactg cctcctcgac cccggcct
//
