LOCUS       sequence-222267-        3387 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor for precise CRISPR directed genomic integration using the 
            GeneWeld method.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Wierson WA, Welker JM, Almeida MP, Mann CM, Webster DA, Torrie ME, 
            Weiss TJ, Kambakam S, Vollbrecht MK, Lan M, McKeighan KC, Levey J, 
            Ming Z, Wehmeier A, Mikelson CS, Haltom JA, Kwan KM, Chien CB, 
            Balciunas D, Ekker SC, Clark KJ, Webber BR, Moriarity BS, Solin SL, 
            Carlson DF, Dobbs DL, McGrail M, Essner J
  TITLE     Efficient targeted integration directed by short homology in 
            zebrafish and mammalian cells.
  JOURNAL   Elife. 2020 May 15;9. pii: 53968. doi: 10.7554/eLife.53968.
  PUBMED    32412410
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-05-15"; volume: "9. pii: 53968. doi"; pages: " 
            10.7554/eLife.53968"
COMMENT     Sequence Label: pPRISM-Stop-gcry-TagBFP
FEATURES             Location/Qualifiers
     source          1..3387
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1909..1929
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1933..2634
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     polyA_signal    2644..2868
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    2938..3019
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    3143..3224
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 cttctataat gataggtagt aatgattagt aatgagacta gtaattaagt ctcagccacc
       61 gttaactgaa catgtcaaaa cctgtggaga ctgttgagat ttgatgttct gaaaagataa
      121 agcctataaa taaaatgttg cccaaatttc ctgcctgatg tttttctttg tctttgctac
      181 atggctttgc tgctcggatc ggctcactct gtgtatgcca cgttcacttt gtactctcct
      241 tctcacggta ggtttattat ttttagatgt gcagttagtt tctgtgaaat aacacaccac
      301 acactgatat tgtctgtgca ttgacttggt gagtgcacat tgtttttgat cttgacatat
      361 ttatatttga ttgatcaggt gaactgtgtg aatctaaagt gctccataca gatgttctgc
      421 attgaaaata ttctcatttt attagtggaa gtgagtgtat gccacatcca atcaatttca
      481 gcaaacaccc cagtatgatt taatgcaaaa aaatgaaggt atcaaacacg cattactact
      541 ttgcagttaa atatttaaca tttattccaa cacgaaaaaa agcagtaaat aacactttga
      601 caaacacgtc aggacatctt atttttgtca ccctcacagg caatttagta taatatatta
      661 tatatatata tatatcatat aataatattc agtataatat atatatatat atcatattat
      721 aatattcagt ataatataaa acacaaacac atatatgtat aatataatat aacattttta
      781 tttattgaga tgcctctatg gaccgtgtta taagaagtaa agatcaggag aagtaaacat
      841 gaagtgtaat tatgaatact gatgttaaat taagctatga tgagttttca ctgttaattt
      901 accatctcaa ttaaatgttg atgcctccat gaccaagtta agcagatgag actgagacaa
      961 ctgtagaaga caagatgttc actttgctga atatagctgg cttgacagtt atctatgact
     1021 ctataaatat atatatattt ttttttttat aaaatgattt atttataact atatatccat
     1081 ttctcagaca ggtgcttcat atccctcact cccgtagctg tccatgctgg atctgtcccc
     1141 gttgttttta aaaagctaaa taagttatta acatgactgc atccagcgag ccaaacctgt
     1201 ctggtgtaca gctaccagag aagcttgaga tcctagtcac cggcgttcct agttctttaa
     1261 actgttacaa agtgtttgga ccagcaactg aattgcactg taaattacca catcagggtc
     1321 tccggggtca acgctgctaa gcaatggact ggaaaatttg tgacattgag tttttggaat
     1381 aactgggaat gctggataac tctttgggta aagctatgga acaaaagtac cagcatgaac
     1441 taagaaatga ctgtcacagc acaacatcaa acacaatgca agacaaaggt tctctgcttt
     1501 tgccttactt gacagaaaaa gtaagctcta ggttcatcgc atgcagacag cagcaataca
     1561 tgacatatat aaaggctggc tcttttctcc cttccattga actgaaactt ccactcagtc
     1621 agacttgcgg gggatccaca gcctgactaa ctcgacaaac ccccccaaac ctaaggtgag
     1681 ttgatcttta agctttttac attttcagct cgcatatatc aattcgcgcg cattgtcaca
     1741 ctagcaccca ctagcggtca gactgcagat tgcagcacga aacaggaagc tgactccaca
     1801 tggtcacatg ctcactgaag tgttgacttc cctgacagct gtgcactttc taaaccggtt
     1861 ttctcattca tttacagttc agccggaatt cacctcgaga ccatggctcc aaagaagaag
     1921 cgtaaggtaa ccatgagcga gctgattaag gagaacatgc acatgaagct gtacatggag
     1981 ggcaccgtgg acaaccatca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag
     2041 ggcacccaga ccatgagaat caaggtggtc gagggcggcc ctctcccctt cgccttcgac
     2101 atcctggcta ctagcttcct ctacggcagc aagaccttca tcaaccacac ccagggcatc
     2161 cccgacttct tcaagcagtc cttccctgag ggcttcacat gggagagagt caccacatac
     2221 gaagacgggg gcgtgctgac cgctacccag gacaccagcc tccaggacgg ctgcctcatc
     2281 tacaacgtca agatcagagg ggtgaacttc acatccaacg gccctgtgat gcagaagaaa
     2341 acactcggct gggaggcctt caccgagacg ctgtaccccg ctgacggcgg cctggaaggc
     2401 agaaacgaca tggccctgaa gctcgtgggc gggagccatc tgatcgcaaa catcaagacc
     2461 acatatagat ccaagaaacc cgctaagaac ctcaagatgc ctggcgtcta ctatgtggac
     2521 tacagactgg aaagaatcaa ggaggccaac aacgagacct acgtcgagca gcacgaggtg
     2581 gcagtggcca gatactgcga cctccctagc aaactggggc acaagctcaa ttaaagctga
     2641 gatctgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     2701 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     2761 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     2821 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     2881 aaagaaccaa agggcgaatt aattcattga tgagtttgga caaaccacaa ctagaatgca
     2941 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     3001 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
     3061 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggtc attgatgagt
     3121 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
     3181 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
     3241 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc
     3301 tctacaaatg tggtcattca gatcaattaa ccctcacccg ggctagcgat gcatgggagc
     3361 tctgacgtac gttgtctagc aaggaag
//
