LOCUS       sequence-222276-        3276 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor for precise CRISPR directed genomic integration using the 
            GeneWeld method.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Wierson WA, Welker JM, Almeida MP, Mann CM, Webster DA, Torrie ME, 
            Weiss TJ, Kambakam S, Vollbrecht MK, Lan M, McKeighan KC, Levey J, 
            Ming Z, Wehmeier A, Mikelson CS, Haltom JA, Kwan KM, Chien CB, 
            Balciunas D, Ekker SC, Clark KJ, Webber BR, Moriarity BS, Solin SL, 
            Carlson DF, Dobbs DL, McGrail M, Essner J
  TITLE     Efficient targeted integration directed by short homology in 
            zebrafish and mammalian cells.
  JOURNAL   Elife. 2020 May 15;9. pii: 53968. doi: 10.7554/eLife.53968.
  PUBMED    32412410
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-05-15"; volume: "9. pii: 53968. doi"; pages: " 
            10.7554/eLife.53968"
COMMENT     Sequence Label: pPRISM-Stop-cmlc2-TagBFP
FEATURES             Location/Qualifiers
     source          1..3276
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1798..1818
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1822..2523
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     polyA_signal    2533..2757
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    2827..2908
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    3032..3113
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 cttctataat gataggtagt aatgattagt aatgagacta gtaattaagt ctcagccacc
       61 gttaactgaa catgtcaaaa cctgtggaga ctgttgagat ttgatgttct gaaaagataa
      121 agcctataaa taaaatgttg cccaaatttc ctgcctgatg tttttctttg tctttgctac
      181 atggctttgc tgctcggatc ggctcactct gtgtatgcca cgttcacttt gtactctcct
      241 tctcacggta ggtttattat ttttagatgt gcagttagtt tctgtgaaat aacacaccac
      301 acactgatat tgtctgtgca ttgacttggt gagtgcacat tgtttttgat cttgacatat
      361 ttatatttga ttgatcaggt gaactgtgtg aatctaaagt gctccataca gatgttctgc
      421 attgaaaata ttctcatttt attagtggaa gtgagtgtat gccacatcca atcaatttca
      481 gcaaacaccc cagtatgatt taatgcaaaa aaatgaaggt atcaaacacg cattactact
      541 ttgcagttaa atatttaaca tttattccaa cacgaaaaaa agcagtaaat aacactttga
      601 caaacacgtc aggacatctt atttttgtca ccctcacagg caatttagta taatatatta
      661 tatatatata tatatcatat aataatattc agtataatat atatatatat atcatattat
      721 aatattcagt ataatataaa acacaaacac atatatgtat aatataatat aacattttta
      781 tttattgaga tgcctctatg gaccgtgtta taagaagtaa agatcaggag aagtaaacat
      841 gaagtgtaat tatgaatact gatgttaaat taagctatga tgagttttca ctgttaattt
      901 accatctcaa ttaaatgttg atgcctccat gaccaagtta agcagatgag actgagacaa
      961 ctgtagaaga caagatgttc actttgctga atatagctgg cttgacagtt atctatgact
     1021 ctataaatat atatatattt ttttttttat aaaatgattt atttataact atatatccat
     1081 ttctcagaca ggtgcttcat atccctcact cccgtagctg tccatgctgg atctgtcccc
     1141 gttgttttta aaaagctaaa taagttatta acatgactgc atccagcgag ccaaacctgt
     1201 ctggtgtaca gctaccagag aagcttgaga tcctagtcac cggcggcatt catccatcct
     1261 tttcatccct caaatctctc attcacgtcc ccctccccat ctgcacactt tatctcattt
     1321 tccaccctgc tggaatctga gcacttgtgc agttatcagg gctcctgtat ttaggaggct
     1381 ctgggtgtcc atgtagggga cgaacagaaa cactgcagac ctttatagaa gaacaaatga
     1441 taagagtcct catacataaa gactccatta gaaacgtcag tgacccagga gcccagacca
     1501 acagcaaagc agacagtgaa ggatccacag cctgactaac tcgacaaacc cccccaaacc
     1561 taaggtgagt tgatctttaa gctttttaca ttttcagctc gcatatatca attcgcgcgc
     1621 attgtcacac tagcacccac tagcggtcag actgcagatt gcagcacgaa acaggaagct
     1681 gactccacat ggtcacatgc tcactgaagt gttgacttcc ctgacagctg tgcactttct
     1741 aaaccggttt tctcattcat ttacagttca gccggaattc acctcgagac catggctcca
     1801 aagaagaagc gtaaggtaac catgagcgag ctgattaagg agaacatgca catgaagctg
     1861 tacatggagg gcaccgtgga caaccatcac ttcaagtgca catccgaggg cgaaggcaag
     1921 ccctacgagg gcacccagac catgagaatc aaggtggtcg agggcggccc tctccccttc
     1981 gccttcgaca tcctggctac tagcttcctc tacggcagca agaccttcat caaccacacc
     2041 cagggcatcc ccgacttctt caagcagtcc ttccctgagg gcttcacatg ggagagagtc
     2101 accacatacg aagacggggg cgtgctgacc gctacccagg acaccagcct ccaggacggc
     2161 tgcctcatct acaacgtcaa gatcagaggg gtgaacttca catccaacgg ccctgtgatg
     2221 cagaagaaaa cactcggctg ggaggccttc accgagacgc tgtaccccgc tgacggcggc
     2281 ctggaaggca gaaacgacat ggccctgaag ctcgtgggcg ggagccatct gatcgcaaac
     2341 atcaagacca catatagatc caagaaaccc gctaagaacc tcaagatgcc tggcgtctac
     2401 tatgtggact acagactgga aagaatcaag gaggccaaca acgagaccta cgtcgagcag
     2461 cacgaggtgg cagtggccag atactgcgac ctccctagca aactggggca caagctcaat
     2521 taaagctgag atctgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     2581 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     2641 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     2701 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
     2761 ctgaggcgga aagaaccaaa gggcgaatta attcattgat gagtttggac aaaccacaac
     2821 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     2881 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
     2941 ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtca
     3001 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
     3061 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
     3121 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca
     3181 agtaaaacct ctacaaatgt ggtcattcag atcaattaac cctcacccgg gctagcgatg
     3241 catgggagct ctgacgtacg ttgtctagca aggaag
//
