LOCUS       sequence-22230-e         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  TITLE     pIND-miR30
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pIND-EGFP
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     201..222
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      264..273
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(775..796)
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
ORIGIN
        1 catggcggac ttgaagaagt cgtgctgctt catgtggtcg gggtagcggc tgaagcactg
       61 cacgccgtag gtcagggtgg tcacgagggt gggccagggc acgggcagct tgccggtggt
      121 gcagatgaac ttcagggtca gcttgccgta ggtggcatcg ccctcgccct cgccggacac
      181 gctgaacttg tggccgttta cgtcgccgtc cagctcgacc aggatgggca ccaccccggt
      241 gaacagctcc tcgcccttgc tcaccatggt ggcgaccggt ggatccacag tcttctgttg
      301 tcctttcttc ccgatcagcg atttgtggat gtgtgggatg acaccaccac cagcaatggt
      361 agctttgatc agagaatcca attcttcatc tccacgtata gcaagctgca agtgacgagg
      421 ggtgatacgc tttaccttta agtcttttga cgcatttcct gccaactcaa gtacctctgc
      481 ggtgaggtac tccaggatgg ctgcgctgta cacagcggcg gtcgcgccca cacgtccgtg
      541 gctggttgtc ctagatttca ggtgtcgatg aatacggccc acagggaact gcaagccggc
      601 tcgctgcgag cgggaaaccg cctttgtctt ggcctttccg gagtcctttc cagccttacc
      661 gccagccatg gtctcgagat ctgagtccgg tagcgctagc tgtgtgtgag ttcttctttc
      721 tcggtaactt gttgaaagta ttcagagttc tcttcttgta ttcaataatt acttcttggc
      781 agatttcagt agttgcagtt gatttacttg gttgctggtt acttttaatt gattcacttt
      841 aactt
//
