LOCUS       sequence-222358-       13326 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid encoding the cytosolic localization of the FRET 
            semi-synthetic biosensor measuring the ratio NADPH/NADP+ in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 13326)
  AUTHORS   Sallin O, Reymond L, Gondrand C, Raith F, Koch B, Johnsson K
  TITLE     Semisynthetic biosensors for mapping cellular concentrations of 
            nicotinamide adenine dinucleotides.
  JOURNAL   eLife. 2018 May 29;7. pii: 32638. doi: 10.7554/eLife.32638.
  PUBMED    29809136
REFERENCE   2  (bases 1 to 13326)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2018-05-29"; volume: "7. pii: 32638. doi"; pages: " 
            10.7554/eLife.32638"
COMMENT     Sequence Label: pEBTet_NADP-Snifit-cyto
FEATURES             Location/Qualifiers
     source          1..13326
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(865..883)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        951..1055
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1056..1124,1125..1916)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1274..1293)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2087..2675
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2576..2595
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(2864..2884)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2882..3179
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3256..3855
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3256..3275)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3752..3772
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    3946..4080
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4218..4237)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        4514..4775
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     intron          4892..5464
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     5472..5491
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     5519..5538
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5519..5537
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5548..6168
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(5621..5640)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     polyA_signal    6210..6331
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        6386..6765
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6766..6969
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6920..6940
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    6971..6989
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6992..7010
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     7020..7044
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             7936..8820
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEISG"
     primer_bind     complement(9551..9568)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9557..9668
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10209..11998
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 ctcctgcccc tcctgcccct cctgcccctc ctcctgctcc tgcccctcct cctgctcctg
       61 cccctcctgc ccctcctgcc cctcctgccc ctcctcctgc tcctgcccct cctcctgctc
      121 ctgcccctcc tgctcctgcc cctcccgctc ctgctcctgc tcctgttcca ccgtgggtcc
      181 ctttgcagcc aatgcaactt ggacgttttt ggggtctccg gacaccatct ctatgtcttg
      241 gccctgatcc tgagccgccc ggggctcctg gtcttccgcc tcctcgtcct cgtcctcttc
      301 cccgtcctcg tccatggtta tcaccccctc ttctttgagg tccactgccg ccggagcctt
      361 ctggtccaga tgtgtctccc ttctctccta ggccatttcc aggtcctgta cctggcccct
      421 cgtcagacat gattcacact aaaagagatc aatagacatc tttattagac gacgctcagt
      481 gaatacaggg agtgcagact cctgccccct ccaacagccc ccccaccctc atccccttca
      541 tggtcgctgt cagacagatc caggtctgaa aattccccat cctccgaacc atcctcgtcc
      601 tcatcaccaa ttactcgcag cccggaaaac tcccgctgaa catcctcaag atttgcgtcc
      661 tgagcctcaa gccaggcctc aaattcctcg tccccctttt tgctggacgg tagggatggg
      721 gattctcggg acccctcctc ttcctcttca aggtcaccag acagagatgc tactggggca
      781 acggaagaaa agctgggtgc ggcctgtgag gatcagctta tcgatgataa gctgtcaaac
      841 atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
      901 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
      961 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     1021 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     1081 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     1141 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     1201 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     1261 cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac
     1321 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     1381 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     1441 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     1501 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     1561 aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc
     1621 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     1681 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     1741 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     1801 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     1861 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     1921 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     1981 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     2041 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     2101 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     2161 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     2221 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     2281 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     2341 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     2401 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     2461 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     2521 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     2581 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     2641 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     2701 ggttcctggc cttttgctgg ccttgaagct gtccctgatg gtcgtcatct acctgcctgg
     2761 acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg
     2821 ggaaggccat ccagcctcgc gtcgaactag atgatccggc tgtggaatgt gtgtcagtta
     2881 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     2941 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     3001 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     3061 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     3121 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     3181 gggtgaccgc cacgaccggt gccgccacca tcccctgacc cacgcccctg acccctcaca
     3241 aggagacgac cttccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     3301 gtcccccggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     3361 accgtcgacc ccgaccgcca catcgaacgc gtcaccgagc tgcaagaact cttcctcacg
     3421 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     3481 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     3541 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     3601 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     3661 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     3721 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     3781 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     3841 aagcccggtg cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga
     3901 cccggtccga cgcggcccga cgggtccgag gggggtcgac ctcgaaactt gtttattgca
     3961 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     4021 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
     4081 tgatccgaac cccttcctcg accaattctc atgtttgaca gcttatcatc gcagatccgg
     4141 gcaacgttgt tgccattgct cgaccaattc tcatgtttga cagcttatca tcgcagatcc
     4201 gtatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc
     4261 cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg
     4321 caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt
     4381 cgcgatgtac gggccagata tacgcgtatc tgaggggact agggtgtgtt taggcgaaaa
     4441 gcggggcttc ggttgtacgc ggttaggagt cccctcagga tatagtagtt tcgcttttgc
     4501 atagggaggg ggaaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga
     4561 tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag
     4621 taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac
     4681 gaaccactga attccgcatt gcagagatat tgtatttaag tgcctagctc gatacaataa
     4741 acgccatttg accattcacc acattggtgt gcacctccaa gctggccggg gatcctctag
     4801 ggcctctgag ctattccaga agtagtgaag aggctttttt ggaggcctag gcttttgcaa
     4861 aaagctccgg atcgatcctg agaacttcag ggtgagtttg gggacccttg attgttcttt
     4921 ctttttcgct attgtaaaat tcatgttata tggagggggc aaagttttca gggtgttgtt
     4981 tagaatggga agatgtccct tgtatcacca tggaccctca tgataatttt gtttctttca
     5041 ctttctactc tgttgacaac cattgtctcc tcttattttc ttttcatttt ctgtaacttt
     5101 ttcgttaaac tttagcttgc atttgtaacg aatttttaaa ttcacttttg tttatttgtc
     5161 agattgtaag tactttctct aatcactttt ttttcaaggc aatcagggta tattatattg
     5221 tacttcagca cagttttaga gaacaattgt tataattaaa tgataaggta gaatatttct
     5281 gcatataaat tctggctggc gtggaaatat tcttattggt agaaacaact acatcctggt
     5341 catcatcctg cctttctctt tatggttaca atgatataca ctgtttgaga tgaggataaa
     5401 atactctgag tccaaaccgg gcccctctgc taaccatgtt catgccttct tctttttcct
     5461 acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattgta
     5521 atacgactca ctatagggcg aattgatatg tctagattag ataaaagtaa agtgattaac
     5581 agcgcattag agctgcttaa tgaggtcgga atcgaaggtt taacaacccg taaactcgcc
     5641 cagaagctag gtgtagagca gcctacattg tattggcatg taaaaaataa gcgggctttg
     5701 ctcgacgcct tagccattga gatgttagat aggcaccata ctcacttttg ccctttagaa
     5761 ggggaaagct ggcaagattt tttacgtaat aacgctaaaa gttttagatg tgctttacta
     5821 agtcatcgcg atggagcaaa agtacattta ggtacacggc ctacagaaaa acagtatgaa
     5881 actctcgaaa atcaattagc ctttttatgc caacaaggtt tttcactaga gaatgcatta
     5941 tatgcactca gcgctgtggg gcattttact ttaggttgcg tattggaaga tcaagagcat
     6001 caagtcgcta aagaagaaag ggaaacacct actactgata gtatgccgcc attattacga
     6061 caagctatcg aattatttga tcaccaaggt gcagagccag ccttcttatt cggccttgaa
     6121 ttgatcatat gcggattaga aaaacaactt aaatgtgaaa gtgggtccgc gtacagcgga
     6181 tcccgggaat tcagatctta ttaaagcaga acttgtttat tgcagcttat aatggttaca
     6241 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     6301 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gtcgaatcca tggcgcagaa
     6361 ctggtaggta tggaagatcc gcgttgacat tgattattga ctagttatta atagtaatca
     6421 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     6481 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     6541 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     6601 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     6661 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     6721 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
     6781 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     6841 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     6901 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
     6961 agcagagctc tccctatcag tgatagagat ctccctatca gtgatagaga tcgtcgacga
     7021 gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat
     7081 agaagacacc gggaccgatc cagcctccgg actctagcgt ttaaacttaa gcttaccacc
     7141 atggagggcg ggctggggcg tgctgtgtgc ttgctgaccg gggcctcccg cggcttcggc
     7201 cggacgctgg ccccgctcct ggcctcgctg ctgtcgcccg gctccgtgct tgtccttagc
     7261 gcccgcaacg acgaggcact gcgccagctg gaggccgagc tgggcgccga gcggtctggc
     7321 ctgcgcgtgg tgcgggtgcc cgccgacctg ggcgccgagg ccggcttgca gcagctgctc
     7381 ggcgccctgc gcgagctccc ccggcccaag gggctgcagc gactgctgct tatcaacaac
     7441 gcgggctctc ttggggatgt gtccaaaggc ttcgtggacc tgagtgactc cactcaagtg
     7501 aacaactact gggcactgaa cttgacctcc atgctctgcc tgacttccag cgtcctgaag
     7561 gccttcccgg acagtcctgg cctcaacaga accgtggtta acatctcgtc cctctgtgcc
     7621 ctgcaacctt tcaaaggctg ggcgctgtac tgtgcaggaa aggctgctcg tgatatgctg
     7681 ttccaggtcc tggcgctgga ggaacctaat gtgagggtgc tgaactatgc cccaggtcct
     7741 ctggacacag acatgcagca gttggcccgg gagacctccg tggacccaga catgcgaaaa
     7801 gggctgcagg agctgaaggc aaaggggaag ctggtggatt gcaaggtgtc agcccagaaa
     7861 ctgctgagct tactggaaaa ggacgagttc aagtctggag cccacgtgga cttctatgac
     7921 aaagaattcg gatccgaaat cggtactggc tttccattcg acccccatta tgtggaagtc
     7981 ctgggcgagc gcatgcacta cgtcgatgtt ggtccgcgcg atggcacccc tgtgctgttc
     8041 ctgcacggta acccgacctc ctcctacgtg tggcgcaaca tcatcccgca tgttgcaccg
     8101 acccatcgct gcattgctcc agacctgatc ggtatgggca aatccgacaa accagacctg
     8161 ggttatttct tcgacgacca cgtccgcttc atggatgcct tcatcgaagc cctgggtctg
     8221 gaagaggtcg tcctggtcat tcacgactgg ggctccgctc tgggtttcca ctgggccaag
     8281 cgcaatccag agcgcgtcaa aggtattgca tttatggagt tcatccgccc tatcccgacc
     8341 tgggacgaat ggccagaatt tgcccgcgag accttccagg ccttccgcac caccgacgtc
     8401 ggccgcaagc tgatcatcga tcagaacgtt tttatcgagg gtacgctgcc gatgggtgtc
     8461 gtccgcccgc tgactgaagt cgagatggac cattaccgcg agccgttcct gaatcctgtt
     8521 gaccgcgagc cactgtggcg cttcccaaac gagctgccaa tcgccggtga gccagcgaac
     8581 atcgtcgcgc tggtcgaaga atacatggac tggctgcacc agtcccctgt cccgaagctg
     8641 ctgttctggg gcaccccagg cgttctgatc ccaccggccg aagccgctcg cctggccaaa
     8701 agcctgccta actgcaaggc tgtggacatc ggcccgggtc tgaatctgct gcaagaagac
     8761 aacccggacc tgatcggcag cgagatcgcg cgctggctgt ctactctgga gatttccggt
     8821 agcggccgcc ctcctcctcc gccaccgcca ccaccgccgc cgccgccacc accacctcct
     8881 ccgcctcctc caccaccacc gccaccgcct ccaccacctg gcggccgctc tcggtctctc
     8941 gagatggata aagactgcga aatgaaacgc actaccctgg acagcccgct gggtaaactg
     9001 gaactgagcg gttgcgagca gggcctgcac gaaatcattt tcctgggcaa aggtacgagc
     9061 gctgcggatg cggtcgaagt accggctccg gctgcggttc tgggtggccc ggagccgctg
     9121 atgcaggcta ccgcatggct gaacgcatat tttcaccagc cagaggcgat tgaagagttc
     9181 cctgtaccgg ccctgcatca cccggtattt cagcaggaat cctttacccg tcaggtactg
     9241 tggaaactgc tgaaagtagt gaaattcggc gaggtgatct cctattccca tctggcggcg
     9301 ctggctggca acccagcggc aactgcggcg gtgaaaaccg ctctgtctgg taatccggtg
     9361 ccaattctga tcccgtgcca ccgtgtggtt cagggcgacc tggacgtagg tggctacgaa
     9421 ggcggcctgg ctgtgaaaga atggctgctg gcgcacgaag gtcaccgtct gggtaaacca
     9481 ggcctgggct aaagctagcg gcgcgcccgg ccgctcgagt ctagagggcc cgtttaaacc
     9541 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     9601 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     9661 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     9721 agcaaggggg aggattggga agacaatagc aggctagagg atcgatcccc gccccggacg
     9781 aactaaacct gactacgaca tctctgcccc ttcttcgcgg ggcagtgcat gtaatccctt
     9841 cagttggttg gtacaacttg ccaactgggc cctgttccac atgtgacacg gggggggacc
     9901 aaacacaaag gggttctctg actgtagttg acatccttat aaatggatgt gcacatttgc
     9961 caacactgag tggctttcat cctggagcag actttgcagt ctgtggactg caacacaaca
    10021 ttgcctttat gtgtaactct tggctgaagc tcttacacca atgctggggg acatgtacct
    10081 cccaggggcc caggaagact acgggaggct acaccaacgt caatcagagg ggcctgtgta
    10141 gctaccgata agcggaccct caagagggca ttagcaatag tgtttataag gcccccttgt
    10201 taaccctaaa cgggtagcat atgcttcccg ggtagtagta tatactatcc agactaaccc
    10261 taattcaata gcatatgtta cccaacggga agcatatgct atcgaattag ggttagtaaa
    10321 agggtcctaa ggaacagcga tatctcccac cccatgagct gtcacggttt tatttacatg
    10381 gggtcaggat tccacgaggg tagtgaacca ttttagtcac aagggcagtg gctgaagatc
    10441 aaggagcggg cagtgaactc tcctgaatct tcgcctgctt cttcattctc cttcgtttag
    10501 ctaatagaat aactgctgag ttgtgaacag taaggtgtat gtgaggtgct cgaaaacaag
    10561 gtttcaggtg acgcccccag aataaaattt ggacgggggg ttcagtggtg gcattgtgct
    10621 atgacaccaa tataaccctc acaaacccct tgggcaataa atactagtgt aggaatgaaa
    10681 cattctgaat atctttaaca atagaaatcc atggggtggg gacaagccgt aaagactgga
    10741 tgtccatctc acacgaattt atggctatgg gcaacacata atcctagtgc aatatgatac
    10801 tggggttatt aagatgtgtc ccaggcaggg accaagacag gtgaaccatg ttgttacact
    10861 ctatttgtaa caaggggaaa gagagtggac gccgacagca gcggactcca ctggttgtct
    10921 ctaacacccc cgaaaattaa acggggctcc acgccaatgg ggcccataaa caaagacaag
    10981 tggccactct tttttttgaa attgtggagt gggggcacgc gtcagccccc acacgccgcc
    11041 ctgcggtttt ggactgtaaa ataagggtgt aataacttgg ctgattgtaa ccccgctaac
    11101 cactgcggtc aaaccacttg cccacaaaac cactaatggc accccgggga atacctgcat
    11161 aagtaggtgg gcgggccaag ataggggcgc gattgctgcg atctggagga caaattacac
    11221 acacttgcgc ctgagcgcca agcacagggt tgttggtcct catattcacg aggtcgctga
    11281 gagcacggtg ggctaatgtt gccatgggta gcatatacta cccaaatatc tggatagcat
    11341 atgctatcct aatctatatc tgggtagcat aggctatcct aatctatatc tgggtagtat
    11401 atgctatcct aatctgtatc cgggtagcat atgctatcct aatagagatt agggtagtat
    11461 atgctatcct aatctatatc tgggtagcat aggctatcct aatctatatc tgggtagtat
    11521 atgctatcct aatctgtatc cgggtagcat atgctatcct aatagagatt agggtagtat
    11581 atgctatcct aatttatatc tgggtagcat atactaccca aatatctgga tagcatatgc
    11641 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagcataggc
    11701 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc
    11761 tatcctaatt tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc
    11821 tatcctaatc tatatctggg tagtatatgc tatcctaatc tgtatccggg tagcatatgc
    11881 tatcctcatg catatacagt cagcatatga tacccagtag tagagtggga gtgctatcct
    11941 ttgcatatgc cgccacctcc caagggggcg tgaattttcg ctgcttgtcc ttttcctgct
    12001 ggttgctccc attcttaggt gaatttaagg aggccaggct aaagccgtcg catgtctgat
    12061 tgctcaccag gtaaatgtcg ctaatgtttt ccaacgcgag aaggtgttga gcgcggagct
    12121 gagtgacgtg acaacatggg tatgcccaat tgccccatgt tgggaggacg aaaatggtga
    12181 caagacagat ggccagaaat acaccaacag cacgcatgat gtctactggg gatttattct
    12241 ttagtgcggg ggaatacacg gcttttaata cgattgaggg cgtctcctaa caagttacat
    12301 cactcctgcc cttcctcacc ctcatctcca tcacctcctt catctccgtc atctccgtca
    12361 tcaccctccg cggcagcccc ttccaccata ggtggaaacc agggaggcaa atctactcca
    12421 tcgtcaaagc tgcacacagt caccctgata ttgcaggtag gagcgggctt tgtcataaca
    12481 aggtccttaa tcgcatcctt caaaacctca gcaaatatat gagtttgtaa aaagaccatg
    12541 aaataacaga caatggactc ccttagcggg ccaggttgtg ggccgggtcc aggggccatt
    12601 ccaaagggga gacgactcaa tggtgtaaga cgacattgtg gaatagcaag ggcagttcct
    12661 cgccttaggt tgtaaaggga ggtcttacta cctccatata cgaacacacc ggcgacccaa
    12721 gttccttcgt cggtagtcct ttctacgtga ctcctagcca ggagagctct taaaccttct
    12781 gcaatgttct caaatttcgg gttggaacct ccttgaccac gatgctttcc aaaccaccct
    12841 ccttttttgc gcctgcctcc atcaccctga ccccggggtc cagtgcttgg gccttctcct
    12901 gggtcatctg cggggccctg ctctatcgct cccgggggca cgtcaggctc accatctggg
    12961 ccaccttctt ggtggtattc aaaataatcg gcttccccta cagggtggaa aaatggcctt
    13021 ctacctggag ggggcctgcg cggtggagac ccggatgatg atgactgact actgggactc
    13081 ctgggcctct tttctccacg tccacgacct ctccccctgg ctctttcacg acttcccccc
    13141 ctggctcttt cacgtcctct accccggcgg cctccactac ctcctcgacc ccggcctcca
    13201 ctacctcctc gaccccggcc tccactgcct cctcgacccc ggcctccacc tcctgctcct
    13261 gcccctcctg ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc tgctcctgcc
    13321 cctcct
//
