LOCUS       sequence-222359-        6938 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  human Mre11 in baculovirus transfer vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6938)
  AUTHORS   Bhaskara V, Dupre A, Lengsfeld B, Hopkins BB, Chan A, Lee JH, Zhang 
            X, Gautier J, Zakian V, Paull TT
  TITLE     Rad50 adenylate kinase activity regulates DNA tethering by 
            Mre11/Rad50 complexes.
  JOURNAL   Mol Cell. 2007 Mar 9;25(5):647-61. doi: 
            10.1016/j.molcel.2007.01.028.
  PUBMED    17349953
REFERENCE   2  (bases 1 to 6938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2007.01"; journalName: "Mol Cell"; date: 
            "2007-03-9- 9"; volume: "25"; issue: "5"; pages: "647-61"
COMMENT     Sequence Label: pTP813
FEATURES             Location/Qualifiers
     source          1..6938
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      2..456
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(89..108)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     298..319
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        482..586
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(587..655,656..1447)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(805..824)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1618..2206
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2107..2126
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2360..2377
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2511..2735
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2802..3335)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3524..3529,3530..3546,3547..3552))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        3904..3995
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     3920..3937
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             6266..6289
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    6428..6562
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6465..6484)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6519..6538
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  6591..6756
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
       61 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      121 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
      181 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
      241 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt cttaatagtg
      301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      421 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
      481 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
      541 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
      601 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
      661 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
      721 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
      781 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
      841 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
      901 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
      961 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     1021 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     1081 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     1141 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     1201 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     1261 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     1321 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     1381 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     1441 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     1501 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     1561 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     1621 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     1681 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     1741 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     1801 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     1861 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     1921 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     1981 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     2041 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     2101 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     2161 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     2221 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     2281 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     2341 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg
     2401 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atagaccagc cgcgtaacct
     2461 ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga
     2521 caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag
     2581 acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt
     2641 tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga
     2701 ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac
     2761 aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg
     2821 tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg
     2881 ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca
     2941 tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact
     3001 gcgagatcat agatatagat ctcactacgc ggctgctcaa acttgggcag aacgtaagcc
     3061 gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta
     3121 cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct
     3181 ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg
     3241 agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg
     3301 ccctgctgcg taacatcgtt gctgctccat aacatcgttg ctgctccata acatcaaaca
     3361 tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa
     3421 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
     3481 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca
     3541 ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac
     3601 cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc
     3661 ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg
     3721 cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt
     3781 ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt
     3841 gttcgcccag gactctagct atagttctag tggttggcta cgtatactcc ggaatattaa
     3901 tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt
     3961 ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca
     4021 ccatcgggcg cggatcccgg tccgaagcgc gcggaattca aaggcctacg tcgacgagct
     4081 cactagtcgc ggccgcgaac ctggtcccag aggagcttga ctgaccataa aaatgagtac
     4141 tgcagatgca cttgatgatg aaaacacatt taaaatatta gttgcaacag atattcatct
     4201 tggatttatg gagaaagatg cagtcagagg aaatgatacg tttgtaacac tcgatgaaat
     4261 tttaagactt gcccaggaaa atgaagtgga ttttattttg ttaggtggtg atctttttca
     4321 tgaaaataag ccctcaagga aaacattaca tacctgcctc gagttattaa gaaaatattg
     4381 tatgggtgat cggcctgtcc agtttgaaat tctcagtgat cagtcagtca actttggttt
     4441 tagtaagttt ccatgggtga actatcaaga tggcaacctc aacatttcaa ttccagtgtt
     4501 tagtattcat ggcaatcatg acgatcccac aggggcagat gcactttgtg ccttggacat
     4561 tttaagttgt gctggatttg taaatcactt tggacgttca atgtctgtgg agaagataga
     4621 cattagtccg gttttgcttc aaaaaggaag cacaaagatt gcgctatatg gtttaggatc
     4681 cattccagat gaaaggctct atcgaatgtt tgtcaataaa aaagtaacaa tgttgagacc
     4741 aaaggaagat gagaactctt ggtttaactt atttgtgatt catcagaaca ggagtaaaca
     4801 tggaagtact aacttcattc cagaacaatt tttggatgac ttcattgatc ttgttatctg
     4861 gggccatgaa catgagtgta aaatagctcc aaccaaaaat gaacaacagc tgttttatat
     4921 ctcacaacct ggaagctcag tggttacttc tctttcccca ggagaagctg taaagaaaca
     4981 tgttggtttg ctgcgtatta aagggaggaa gatgaatatg cataaaattc ctcttcacac
     5041 agtgcggcag tttttcatgg aggatattgt tctagctaat catccagaca tttttaaccc
     5101 agataatcct aaagtaaccc aagccataca aagcttctgt ttggagaaga ttgaagaaat
     5161 gcttgaaaat gctgaacggg aacgtctggg taattctcac cagccagaga agcctcttgt
     5221 acgactgcga gtggactata gtggaggttt tgaacctttc agtgttcttc gctttagcca
     5281 gaaatttgtg gatcgggtag ctaatccaaa agacattatc cattttttca ggcatagaga
     5341 acaaaaggaa aaaacaggag aagagatcaa ctttgggaaa cttatcacaa agccttcaga
     5401 aggaacaact ttaagggtag aagatcttgt aaaacagtac tttcaaaccg cagagaagaa
     5461 tgtgcagctc tcactgctaa cagaaagagg gatgggtgaa gcagtacaag aatttgtgga
     5521 caaggaggag aaagatgcca ttgaggaatt agtgaaatac cagttggaaa aaacacagcg
     5581 atttcttaaa gaacgtcaca ttgatgccct cgaagacaaa atcgatgagg aggtacgtcg
     5641 tttcagagaa accagacaaa aaaatactaa tgaagaagat gatgaagtcc gtgaggctat
     5701 gaccagggcc agagcactca gatctcagtc agaggagtct gcttctgcct ttagtgctga
     5761 tgaccttatg agtatagatt tagcagaaca gatggctaat gactctgatg atagcatctc
     5821 agcagcaacc aacaaaggaa gaggccgagg aagaggtcga agaggtggaa gagggcagaa
     5881 ttcagcatcg agaggagggt ctcaaagagg aagagcagac actggtctgg agacttctac
     5941 ccgtagcagg aactcaaaga ctgctgtgtc agcatctaga aatatgtcta ttatagatgc
     6001 ctttaaatct acaagacagc agccttcccg aaatgtcact actaagaatt attcagaggt
     6061 gattgaggta gatgaatcag atgtggaaga agacattttt cctaccactt caaagacaga
     6121 tcaaaggtgg tccagcacat catccagcaa aatcatgtcc cagagtcaag tatcgaaagg
     6181 ggttgatttt gaatcaagtg aggatgatga tgatgatcct tttatgaaca ctagttcttt
     6241 aagaagaaat agaagactcg gagcagacta caaggacgat gacgataaat agggtaccaa
     6301 gcttgtcgag aagtactaga ggatcataat cagccatacc acatttgtag aggttttact
     6361 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     6421 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     6481 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     6541 tgtatcttat catgtctgga tctgatcact gcttgagcct aggagatccg aaccagataa
     6601 gtgaaatcta gttccaaact attttgtcat ttttaatttt cgtattagct tacgacgcta
     6661 cacccagttc ccatctattt tgtcactctt ccctaaataa tccttaaaaa ctccatttcc
     6721 acccctccca gttcccaact attttgtccg cccacagcgg ggcatttttc ttcctgttat
     6781 gtttttaatc aaacatcctg ccaactccat gtgacaaacc gtcatcttcg gctacttttt
     6841 ctctgtcaca gaatgaaaat ttttctgtca tctcttcgtt attaatgttt gtaattgact
     6901 gaatatcaac gcttatttgc agcctgaatg gcgaatgg
//
