LOCUS       sequence-22237-e         694 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Jin J, Li X, Gygi SP, Harper JW
  TITLE     Dual E1 activation systems for ubiquitin differentially regulate E2 
            enzyme charging.
  JOURNAL   Nature. 2007 Jun 28. 447(7148):1135-8.
  PUBMED    17597759
REFERENCE   2  (bases 1 to 694)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2007 Jun 28. 447(7148):1135-8."
COMMENT     Sequence Label: pDEST17-UbE2H
FEATURES             Location/Qualifiers
     source          1..694
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             42..59
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             63..95
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(99..107,108..122)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     protein_bind    150..174
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tgtttaactt taagaaggag atatacatag tgcggggttc tcatcatcat catcatcatg
       61 gtatggctag catgactggt ggacagcaaa tgggtcggga tctgtacgac gatgacgata
      121 aggatccgag ctcgagatct gcagatcaaa caagtttgta caaaaaagtt ggcatgtcat
      181 ctcccagtcc gggcaagagg cggatggaca cggacgtggt caagctcatc gagagtaaac
      241 atgaggttac gatcctggga ggacttaatg aatttgtagt gaagttttat ggaccacaag
      301 gaacaccata tgaaggcgga gtatggaaag ttagagtgga cctacctgat aaataccctt
      361 tcaaatctcc atctatagga ttcatgaata aaattttcca tcccaacatt gatgaagcgt
      421 caggaactgt gtgtctagat gtaattaatc aaacttggac agctctctat gatcttacca
      481 atatatttga gtccttcctg cctcagttat tggcctatcc taaccccata gatcctctca
      541 atggtgacgc tgcagccatg tacctccacc gaccagaaga atacaagcag aaaattaaag
      601 agtacatcca gaaatacgcc acggaggagg cgctgaaaga acaggaagag ggtaccgggg
      661 acagctcatc ggagagctct atgtctgact tttc
//
