LOCUS       sequence-22240-e         703 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Jin J, Li X, Gygi SP, Harper JW
  TITLE     Dual E1 activation systems for ubiquitin differentially regulate E2 
            enzyme charging.
  JOURNAL   Nature. 2007 Jun 28. 447(7148):1135-8.
  PUBMED    17597759
REFERENCE   2  (bases 1 to 703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2007 Jun 28. 447(7148):1135-8."
COMMENT     Sequence Label: pDEST17-UbE2B
FEATURES             Location/Qualifiers
     source          1..703
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             32..49
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             53..85
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(89..97,98..112)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     protein_bind    140..164
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(666..690)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 taagaaggag atatacatga tgcggggttc tcatcatcat catcatcatg gtatggctag
       61 catgactggt ggacagcaaa tgggtcggga tctgtacgac gatgacgata aggatccgag
      121 ctcgagatct gcagatcaaa caagtttgta caaaaaagca ggctccgcgg ccgccccctt
      181 caccatgtcg accccggccc ggaggaggct catgcgggat ttcaagcggt tacaagagga
      241 cccacctgtg ggtgtcagtg gcgcaccatc tgaaaacaac atcatgcagt ggaatgcagt
      301 tatatttgga ccagaaggga caccttttga agatggtact tttaaactag taatagaatt
      361 ttctgaagaa tatccaaata aaccaccaac tgttaggttt ttatccaaaa tgtttcatcc
      421 aaatgtgtat gctgatggta gcatatgttt agatatcctt cagaatcgat ggagtccaac
      481 atatgatgta tcttctatct taacatcaat tcagtctctg ctggatgaac cgaatcctaa
      541 cagtccagcc aatagccagg cagcacagct ttatcaggaa aacaaacgag aatatgagaa
      601 aagagtttcg gccattgttg aacaaagctg gaatgattca taactcgaga agggtgggcg
      661 cgccgaccca gctttcttgt acaaagtggt tcgatctggt acc
//
