LOCUS       sequence-222404-        6475 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  in vivo voltage imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6475)
  AUTHORS   Adam Y, Kim JJ, Lou S, Zhao Y, Xie ME, Brinks D, Wu H, Mostajo-Radji
            MA, Kheifets S, Parot V, Chettih S, Williams KJ, Gmeiner B, Farhi 
            SL, Madisen L, Buchanan EK, Kinsella I, Zhou D, Paninski L, Harvey 
            CD, Zeng H, Arlotta P, Campbell RE, Cohen AE
  TITLE     Voltage imaging and optogenetics reveal behaviour-dependent changes 
            in hippocampal dynamics.
  JOURNAL   Nature. 2019 May 1. pii: 10.1038/s41586-019-1166-7. doi: 
            10.1038/s41586-019-1166-7.
  PUBMED    31043747
REFERENCE   2  (bases 1 to 6475)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2019 May 1. pii: 10.1038/s41586-019-1166-7. doi: 
            10.1038/s41586-019-1166-7."
COMMENT     Sequence Label: YA1629: pAAV_CAG-FLEX-paQuasAr3-s
FEATURES             Location/Qualifiers
     source          1..6475
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     279..301
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     363..382
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    417..450
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    513..546
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(join(799..1509,1510..1512))
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(841..862)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1190..1209
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1449..1470
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(1519..2277)
                     /codon_start=1
                     /product="achaerhodopsin-3 (Arch) derivative with improved 
                     voltage sensitivity (Hochbaum et al., 2014)"
                     /label=QuasAr2
                     /translation="MVSIALQAGYDLLGDGRPETLWLGIGTLLMLIGTFYFLVRGWGVT
                     DKDAREYYAVTILASGIASAAYLSMFFGIGLTEVSVGGEMLDIYYARYAQWLFTTPLLL
                     LHLALLAKVDRVTIGTLVGVDALMIVTGLIGALSHTAIARYSWWLFSTICMIVVLYVLA
                     TSLRSAARERGPEVASTFNTLTALVLVLWTAYPILWIIGTEGAGVVGLGIETLLFMVLD
                     VTAKVGFGFILLRSRAILGDTEAPEPSAGAD"
     regulatory      2274..2283
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(2300..2333)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(2404..2437)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    2479..3067
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2532..2552)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2950..2961)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3110..3231
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3121..3140)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3175..3194
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   3393..3522
                     /label=AAV2 ITR
     rep_origin      complement(3759..4347)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3839..3858)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4518..5309,5310..5378))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5141..5160
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5379..5483)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5509..5964)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5646..5667)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5858..5877
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   6036..6165
                     /label=AAV2 ITR
ORIGIN
        1 ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg
       61 ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctcct
      121 ccgggctgta attagcgctt ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa
      181 agccttgagg ggctccggga gggccctttg tgcgggggga gcggctcggg gctgtccgcg
      241 gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac
      301 cggcggctct agagcctctg ctaaccatgt tcatgccttc ttctttttcc tacagctcct
      361 gggcaacgtg ctggttattg tgctgtctca tcattttggc aaagaattgg atccccataa
      421 cttcgtataa agtatcctat acgaagttat atcaaaatag gaagaccaat gcttcaccat
      481 cgacccgaat tgccaagcat caccatcgac ccataacttc gtataatgta tgctatacga
      541 agttatacta gggatccccc ctcgagtttt acttgtacat tacacttcat tctcataaca
      601 aaagaatctg gtggcttcag ggaagtccgt ggcacagctg atgaagctgt caatgctgga
      661 catgctccgc atgtcgatga cgccctccgt gcgtgcgggc agaggggcca cggggctggg
      721 catgctgctc atctccagct cctctggagg cttgctctgc ggggccatct ccttggtgtt
      781 gaggatgggc tgggactgct tgtacagctc gtccatgccg agagtgatcc cggcggcggt
      841 cacgaactcc agcaggacca tgtgatcgcg cttctcgttg gggtctttgc tcagggcgga
      901 ctggtagctc aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt
      961 ctgctggtag tggtcggcga gctgcacgct gccgtcctcg atgttgtggc ggatcttgaa
     1021 gttcaccttg atgccgttct tctgcttgtc ggccatgata tagacgttgt ggctgttgta
     1081 gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag
     1141 ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt
     1201 gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca tggcggactt
     1261 gaagaagtcg tgctgcttca tgtggtcggg gtagcgggcg aagcacatca ggccgtagcc
     1321 gaaggtggtc acgagggtgg gccagggcac gggcagcttg ccggtggtgc agatgaactt
     1381 cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg ccggacacgc tgaacttgtg
     1441 gccgtttacg tcgccgtcca gctcgaccag gatgggcacc accccggtga acagctcctc
     1501 gcccttgctc accggaggat cggcaccggc actgggttct ggtgcctcgg tgtcgcccag
     1561 aatagcccgg gatctcaaca ggataaagcc aaagccgacc ttggcagtca cgtccaacac
     1621 cataaacagc agagtttcga tgcccaggcc caccacgcca gcgccctcag tgcctatgat
     1681 ccacaggata gggtaagcgg tccacagcac caagaccaga gctgtcaggg tgttaaaggt
     1741 agatgccacc tcggggcccc gctcccttgc agcagatcgc agggatgtag ccagaacata
     1801 gagcaccact atcatgcaaa ttgtagagaa caaccaccaa ctgtatctgg ctatggccgt
     1861 gtggctcaag gctccgatga ggccagtgac gatcatcagg gcgtccacac ccaccagggt
     1921 gccgatggtc acccgatcca ccttagcgag aagggccagg tgcagcagca gaagtggggt
     1981 ggtaaacagc cactgggcgt acctggcata atagatatcc aacatttcgc ccccgacgga
     2041 cacctcagta agcccgatac caaagaacat agacagatat gcggcggatg cgattccgga
     2101 ggccaggata gtcacagcgt aatattcccg ggcatcctta tcggtgactc cccatccgcg
     2161 gaccagaaag tagaaggttc caatcagcat cagcagagtg ccgatgccca gccacagggt
     2221 ttcaggtctg ccgtcaccca gtaggtcgta accagcctgc agagcgatac ttaccatggt
     2281 ggcggtaccg aattctagta taacttcgta taggatactt tatacgaagt tatcattggg
     2341 attcttccta ttttgatcca agcatcacca tcgaccctct agtccagatc tcaccatcga
     2401 cccataactt cgtatagcat acattatacg aagttatgtc cctcgaagag gttcgaattc
     2461 gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     2521 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     2581 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     2641 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     2701 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     2761 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     2821 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     2881 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     2941 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     3001 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     3061 tccccgcatc gataccgtcg acccgggcgg ccgcttcgag cagacatgat aagatacatt
     3121 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
     3181 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
     3241 aattgcattc attttatgtt tcaggttcag ggggagatgt gggaggtttt ttaaagcaag
     3301 taaaacctct acaaatgtgg taaaatcgat aaggatcttc ctagagcatg gctacgtaga
     3361 taagtagcat ggcgggttaa tcattaacta caaggaaccc ctagtgatgg agttggccac
     3421 tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc
     3481 gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcatta atgaatcggc
     3541 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     3601 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     3661 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     3721 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     3781 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     3841 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     3901 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     3961 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     4021 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     4081 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     4141 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     4201 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     4261 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     4321 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     4381 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     4441 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     4501 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     4561 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     4621 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     4681 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     4741 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     4801 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     4861 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     4921 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     4981 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     5041 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     5101 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     5161 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     5221 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     5281 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     5341 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     5401 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     5461 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctaa attgtaagcg
     5521 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     5581 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     5641 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     5701 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
     5761 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     5821 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     5881 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     5941 ttaatgcgcc gctacagggc gcgtcccatt cgccattcag gctgcgcaac tgttgggaag
     6001 ggcgatcggt gcgggcctct tcgctattac gccagctgcg cgctcgctcg ctcactgagg
     6061 ccgcccgggc aaagcccggg cgtcgggcga cctttggtcg cccggcctca gtgagcgagc
     6121 gagcgcgcag agagggagtg gccaactcca tcactagggg ttccttgtag ttaatgatta
     6181 acccgccatg ctacttatct acgtagccat gctctaggaa gagtaccatt gacgtcaata
     6241 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
     6301 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
     6361 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
     6421 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catgg
//
