LOCUS       sequence-22241-e         650 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Jin J, Li X, Gygi SP, Harper JW
  TITLE     Dual E1 activation systems for ubiquitin differentially regulate E2 
            enzyme charging.
  JOURNAL   Nature. 2007 Jun 28. 447(7148):1135-8.
  PUBMED    17597759
REFERENCE   2  (bases 1 to 650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2007 Jun 28. 447(7148):1135-8."
COMMENT     Sequence Label: pET15-UbE2D1
FEATURES             Location/Qualifiers
     source          1..650
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             9..31
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             73..105
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(109..117,118..132)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     protein_bind    160..184
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 aaataatttt tgtttaactt taagaaggag aatatacata tgcggggttc tcatcatcat
       61 catcatcatg gtatggctag catgactggt ggacagcaaa tgggtcggga tctgtacgac
      121 gatgacgata aggatccgag ctcgagatct gcagatcaaa caagtttgta caaaaaagca
      181 ggctccgcgg ccgccccctt caccatggcg ctgaagagga ttcagaaaga attgagtgat
      241 ctacagcgcg atccacctgc tcactgttca gctggacctg tgggagatga cttgttccac
      301 tggcaagcca ctattatggg gcctcctgat agcgcatatc aaggtggagt cttctttctc
      361 actgtacatt ttccgacaga ttatcctttt aaaccaccaa agattgcttt cacaacaaaa
      421 atttaccatc caaacataaa cagtaatgga agtatttgtc tcgatattct gaggtcacaa
      481 tggtcaccag ctctgactgt atcaaaagtt ttattgtcca tatgttctct actttgtgat
      541 cctaatccag atgacccctt agtaccagat atcgcacaaa tctataaatc agacaaagaa
      601 aaatacaaca gacatgcaag agaatggact cagaaatatg caatgtaact
//
