LOCUS       sequence-222418-        1816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor for precise CRISPR directed genomic integration using the 
            GeneWeld method.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Wierson WA, Welker JM, Almeida MP, Mann CM, Webster DA, Torrie ME, 
            Weiss TJ, Kambakam S, Vollbrecht MK, Lan M, McKeighan KC, Levey J, 
            Ming Z, Wehmeier A, Mikelson CS, Haltom JA, Kwan KM, Chien CB, 
            Balciunas D, Ekker SC, Clark KJ, Webber BR, Moriarity BS, Solin SL, 
            Carlson DF, Dobbs DL, McGrail M, Essner J
  TITLE     Efficient targeted integration directed by short homology in 
            zebrafish and mammalian cells.
  JOURNAL   Elife. 2020 May 15;9. pii: 53968. doi: 10.7554/eLife.53968.
  PUBMED    32412410
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-05-15"; volume: "9. pii: 53968. doi"; pages: " 
            10.7554/eLife.53968"
COMMENT     Sequence Label: pGTag-TagRFP-B-actin
FEATURES             Location/Qualifiers
     source          1..1816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             10..66
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             67..780
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
ORIGIN
        1 ggatccggag ccacgaactt ctctctgtta aagcaagcag gagacgtgga agaaaacccc
       61 ggtcctatgg tgtctaaggg cgaggaactg attaaggaga acatgcacat gaagctgtac
      121 atggagggca ccgtgaacaa ccaccacttc aagtgcacat ccgagggcga aggcaagccc
      181 tacgagggca cccagaccat gagaatcaag gtggtcgagg gcggccctct ccccttcgcc
      241 ttcgacatcc tggctaccag cttcatgtac ggcagcagaa ccttcatcaa ccacacccag
      301 ggcatccccg acttctttaa gcagtccttc cctgagggct tcacatggga gagagtcacc
      361 acatacgaag acgggggcgt gctgaccgct acccaggaca ccagcctcca ggacggctgc
      421 ctcatctaca acgtcaagat cagaggggtg aacttcccat ccaacggccc tgtgatgcag
      481 aagaaaacac tcggctggga ggccaacacc gagatgctgt accccgctga cggcggcctg
      541 gaaggcagaa gcgacatggc cctgaagctc gtgggcgggg gccacctgat ctgcaacttc
      601 aagaccacat acagatccaa gaaacccgct aagaacctca agatgcccgg cgtctactat
      661 gtggaccaca gactggaaag aatcaaggag gccgacaaag agacctacgt cgagcagcac
      721 gaggtggctg tggccagata ctgcgacctc cctagcaaac tggggcacaa acttaattga
      781 ggcgcgccac ggactgttac cacttcacgc cgactcaaac tgcgcagaga aacttcaaac
      841 gacaacattg gcatggcttt tgttattttt ggcgcttgac tcaggatcta aaaactggaa
      901 cggtgaaggt gacggcaatg ttttttggca aataagcatc cccgaagttc tacaatgcat
      961 ctgaggactc catgttttgt tttctttagt cattccaaat gtttgttaaa tgcattgttc
     1021 cgaaacttat ttgcctctat gaaggctgcc cagtaattgg gagcatactt aacattgtag
     1081 tattgtatgt aaattatgta acaaaacaat gtctgggttt ttgtactttt cagccttaaa
     1141 atcttgggtt tatttttttt tctttgttcc aaaaaaacga agctttacca ttcaagatgt
     1201 aaaggtttcc attccccctg ggcatattgt aaaagctgtg tggaacgtgg cggtgccaga
     1261 catttggtgg ggccaacctg tacactgact aattcaattc caataaaagt gcacatgtaa
     1321 gacatcctac ccctgtctca tttcctgtct tggctctgag tggcttcaat aaaaatggct
     1381 tttgtgaagt tgcaccatgt taattacatt tcagcacttt cacaccaggt ttttgactaa
     1441 tttgaattta tcgaagtttg accttttttt ttttttttct tcaagctaat ttgaggttat
     1501 caaaccattt cattggagga aacttaatgt ttaaaagcat aaattcgaaa catgattcat
     1561 ttatttaact gcttaatggt gaaacaaact tcaagtaata caccaagtgt tccctcatat
     1621 gaaggtagct aactgcaaac ggccttaact ttccatctta agttgagcta cataaaggct
     1681 ttaacaggaa ggaactgcca gtttaaaagt caaaccacca tgactgatat aaattcttgt
     1741 ccatataacc ttgcgtttca cacaatatag ctatctactg ctaaataaat taccatggcg
     1801 ttgtctagca aggaag
//
