LOCUS       sequence-22244-e         641 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Jin J, Li X, Gygi SP, Harper JW
  TITLE     Dual E1 activation systems for ubiquitin differentially regulate E2 
            enzyme charging.
  JOURNAL   Nature. 2007 Jun 28. 447(7148):1135-8.
  PUBMED    17597759
REFERENCE   2  (bases 1 to 641)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2007 Jun 28. 447(7148):1135-8."
COMMENT     Sequence Label: pDEST17-UbE2D4
FEATURES             Location/Qualifiers
     source          1..641
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             40..57
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             61..93
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(97..105,106..120)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     protein_bind    148..172
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tgtttacttt aagaaggaga tatacatatg cggggttctc atcatcatca tcatcatggt
       61 atggctagca tgactggtgg acagcaaatg ggtcgggatc tgtacgacga tgacgataag
      121 gatccgagct cgagatctgc agatcaaaca agtttgtaca aaaaagcagg ctccgcggcc
      181 gcccccttca ccatggcgct aaagcggatc cagaaggaat taaccgactt gcagagggat
      241 cctcctgccc agtgttctgc aggacctgtc ggtgatgact tgttccactg gcaggccacc
      301 atcatgggcc cgaatgacag tccttaccaa ggaggtgttt tcttcctgac catccacttt
      361 cctacagatt acccgttcaa gcccccaaag gttgctttca caaccaaaat ttatcaccct
      421 aatatcaaca gcaatggcag catctgcctt gatatcctgc ggtctcagtg gtctccagcg
      481 ttgactgtgt caaaagttct cttgtccatc tgctcgctgc tctgcgaccc caaccccgat
      541 gaccccctgg tgccagagat agcacacacc tacaaggccg acagagagaa gtacaacaga
      601 ctagcaagag agtggacaca aaaatatgct atgtaactcg a
//
