LOCUS       sequence-222445-        1757 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor for precise CRISPR directed genomic integration using the 
            GeneWeld method.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Wierson WA, Welker JM, Almeida MP, Mann CM, Webster DA, Torrie ME, 
            Weiss TJ, Kambakam S, Vollbrecht MK, Lan M, McKeighan KC, Levey J, 
            Ming Z, Wehmeier A, Mikelson CS, Haltom JA, Kwan KM, Chien CB, 
            Balciunas D, Ekker SC, Clark KJ, Webber BR, Moriarity BS, Solin SL, 
            Carlson DF, Dobbs DL, McGrail M, Essner J
  TITLE     Efficient targeted integration directed by short homology in 
            zebrafish and mammalian cells.
  JOURNAL   Elife. 2020 May 15;9. pii: 53968. doi: 10.7554/eLife.53968.
  PUBMED    32412410
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-05-15"; volume: "9. pii: 53968. doi"; pages: " 
            10.7554/eLife.53968"
COMMENT     Sequence Label: pGTag-gal4-VP16-B-actin
FEATURES             Location/Qualifiers
     source          1..1757
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             10..66
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             73..510
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="KLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTKR
                     SPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNKD
                     AVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
ORIGIN
        1 ggatccggag ccacgaactt ctctctgtta aagcaagcag gagacgtgga agaaaacccc
       61 ggtccttcta gaaagctact gtcttctatc gaacaagcat gcgatatttg ccgacttaaa
      121 aagctcaagt gctccaaaga aaaaccgaag tgcgccaagt gtctgaagaa caactgggag
      181 tgtcgctact ctcccaaaac caaaaggtct ccgctgacta gggcacatct gacagaagtg
      241 gaatcaaggc tagaaagact ggaacagcta tttctactga tttttcctcg agaagacctt
      301 gacatgattt tgaaaatgga ttctttacag gatataaaag cattgttaac aggattattt
      361 gtacaagata atgtgaataa agatgccgtc acagatagat tggcttcagt ggagactgat
      421 atgcctctaa cattgagaca gcatagaata agtgcgacat catcatcgga agagagtagt
      481 aacaaaggtc aaagacagtt gactgtatcg tcgaggtcga ccccgggaat acagatctct
      541 cgagccgccc ccccgaccga tgtcagcctg ggggacgagc tccacttaga cggcgaggac
      601 gtggcgatgg cgcatgccga cgcgctagac gatttcgatc tggacatgtt gggggacggg
      661 gattccccgg gtccgggatt taccccccac gactccgccc cctacggcgc tctggatatg
      721 taaatcgata cggactgtta ccacttcacg ccgactcaaa ctgcgcagag aaacttcaaa
      781 cgacaacatt ggcatggctt ttgttatttt tggcgcttga ctcaggatct aaaaactgga
      841 acggtgaagg tgacggcaat gttttttggc aaataagcat ccccgaagtt ctacaatgca
      901 tctgaggact ccatgttttg ttttctttag tcattccaaa tgtttgttaa atgcattgtt
      961 ccgaaactta tttgcctcta tgaaggctgc ccagtaattg ggagcatact taacattgta
     1021 gtattgtatg taaattatgt aacaaaacaa tgtctgggtt tttgtacttt tcagccttaa
     1081 aatcttgggt ttattttttt ttctttgttc caaaaaaacg aagctttacc attcaagatg
     1141 taaaggtttc cattccccct gggcatattg taaaagctgt gtggaacgtg gcggtgccag
     1201 acatttggtg gggccaacct gtacactgac taattcaatt ccaataaaag tgcacatgta
     1261 agacatccta cccctgtctc atttcctgtc ttggctctga gtggcttcaa taaaaatggc
     1321 ttttgtgaag ttgcaccatg ttaattacat ttcagcactt tcacaccagg tttttgacta
     1381 atttgaattt atcgaagttt gacctttttt tttttttttc ttcaagctaa tttgaggtta
     1441 tcaaaccatt tcattggagg aaacttaatg tttaaaagca taaattcgaa acatgattca
     1501 tttatttaac tgcttaatgg tgaaacaaac ttcaagtaat acaccaagtg ttccctcata
     1561 tgaaggtagc taactgcaaa cggccttaac tttccatctt aagttgagct acataaaggc
     1621 tttaacagga aggaactgcc agtttaaaag tcaaaccacc atgactgata taaattcttg
     1681 tccatataac cttgcgtttc acacaatata gctatctact gctaaataaa ttaccatggc
     1741 gttgtctagc aaggaag
//
