LOCUS       sequence-22246-e         616 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Jin J, Li X, Gygi SP, Harper JW
  TITLE     Dual E1 activation systems for ubiquitin differentially regulate E2 
            enzyme charging.
  JOURNAL   Nature. 2007 Jun 28. 447(7148):1135-8.
  PUBMED    17597759
REFERENCE   2  (bases 1 to 616)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2007 Jun 28. 447(7148):1135-8."
COMMENT     Sequence Label: pDEST17-UbE2E2
FEATURES             Location/Qualifiers
     source          1..616
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             37..54
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             58..90
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(94..102,103..117)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     protein_bind    145..169
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttactttaag aaggagaata catatagcgg ggttctcatc atcatcatca tcatggtatg
       61 gctagcatga ctggtggaca gcaaatgggt cgggatctgt acgacgatga cgataaggat
      121 ccgagctcga gatctgcaga tcaaacaagt ttgtacaaaa aagcaggctc cgcggccgcc
      181 cccttcacca tgtccactga ggcacaaaga gttgatgaca gtccaagcac tagtggagga
      241 agttccgatg gagatcaacg tgaaagtgtt cagcaagaac cagaaagaga acaagttcag
      301 cccaagaaaa aggagggaaa aatatccagc aaaaccgctg ctaaattgtc aactagtgct
      361 aaaagaattc agaaggaact tgcagaaatc acattggacc ctcctcccaa ctgtagtgct
      421 ggacccaaag gagacaacat ttatgaatgg aggtcaacta tattgggacc cccaggatct
      481 gtctatgaaa ggaggggtgt tctttcttga cattaccttt tcaccagact atccgtttaa
      541 accccctaag gttaccttcc gaacaagaat ctatcactgt aatattaaca gccaaggtgt
      601 gatctgtctg gacatc
//
