LOCUS       sequence-22247-e         672 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Jin J, Li X, Gygi SP, Harper JW
  TITLE     Dual E1 activation systems for ubiquitin differentially regulate E2 
            enzyme charging.
  JOURNAL   Nature. 2007 Jun 28. 447(7148):1135-8.
  PUBMED    17597759
REFERENCE   2  (bases 1 to 672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2007 Jun 28. 447(7148):1135-8."
COMMENT     Sequence Label: pDEST17-UbE2E3
FEATURES             Location/Qualifiers
     source          1..672
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             11..28
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             32..64
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(68..76,77..91)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     protein_bind    119..143
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gcggggttct catcatcatc atcatcatgg tatggctagc atgactggtg gacagcaaat
       61 gggtcgggat ctgtacgacg atgacgataa ggatccgagc tcgagatctg cagatcaaac
      121 aagtttgtac aaaaaagttg gcatgtccag tgataggcaa aggtccgatg atgagagccc
      181 cagcaccagc agtggcagtt cagatgcgga ccagcgagac ccagccgctc cagagcctga
      241 agaacaagag gaaagaaaac cttctgccac ccagcagaag aaaaacacca aactctctag
      301 caaaaccact gctaagttat ccactagtgc taaaagaatt cagaaggagc tagctgaaat
      361 aacccttgat cctcctccta attgcagtgc tgggcctaaa ggagataaca tttatgaatg
      421 gagatcaact atacttggtc caccgggttc tgtatatgaa ggtnggtgtg ttttttctgg
      481 atatcacatt ttcatcagat tatccattta agccaccaaa ggttactttc cgcaccagaa
      541 tctatcactg caacatcaac agtcagggag tcatctgtct ggacatcctt aaagacaact
      601 ggagtcccgc tttgactatt tcaaaggttt tgctgtctat ttgttccctt ttgacagact
      661 gcaaccctgc gg
//
