LOCUS       sequence-222475-        3447 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes glycerol kinase (GUT1) of Y. lipolytica for GoldenMOCS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Egermeier M, Sauer M, Marx H
  TITLE     Golden Gate based metabolic engineering strategy for wild-type 
            strains of Yarrowia lipolytica.
  JOURNAL   FEMS Microbiol Lett. 2019 Jan 29. pii: 5304171. doi: 
            10.1093/femsle/fnz022.
  PUBMED    30698703
REFERENCE   2  (bases 1 to 3447)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS 
            Microbiol Lett. 2019 Jan 29. pii: 5304171. doi: 
            10.1093/femsle/fnz022."
COMMENT     Sequence Label: BB1_23_YlGUT1
FEATURES             Location/Qualifiers
     source          1..3447
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(17..33)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(17..33)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      81..112
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        113..215
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             216..1031
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCLKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(289..308)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1055..1082
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1094..1681)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1174..1193)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1721..1738
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1722..1738
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 atctagtcag cttgatgtca tagctgtttc ctgaggctca atactgacca tttaaatcat
       61 acctgacctc catagcagaa agtcaaaagc ctccgaccgg aggcttttga cttgatcggc
      121 acgtaagagg ttccaacttt caccataatg aaataagatc actaccgggc gtattttttg
      181 agttatcgag attttcagga gctaaggaag ctaaaatgag ccatattcaa cgggaaacgt
      241 cttgcttgaa gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg
      301 ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg attgtatggg aagcccgatg
      361 cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga
      421 tggtcaggct aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc
      481 gtactcctga tgatgcatgg ttactcacca ctgcgatccc agggaaaaca gcattccagg
      541 tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc
      601 gccggttgca ttcgattcct gtttgtaatt gtccttttaa cggcgatcgc gtatttcgtc
      661 tcgctcaggc gcaatcacga atgaataacg gtttggttgg tgcgagtgat tttgatgacg
      721 agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactc ttgccattct
      781 caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg
      841 ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc
      901 ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc
      961 aaaaatatgg tattgataat cctgatatga ataaattgca gtttcacttg atgctcgatg
     1021 agtttttcta atgaggacct aaatgtaatc acctggctca ccttcgggtg ggcctttctg
     1081 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgatgct
     1141 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     1201 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     1261 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     1321 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     1381 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     1441 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     1501 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     1561 tgaagccagt tacctcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     1621 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     1681 agaagatcct ttgattttct accgaagaaa ggcccacccg tgtaaaacga cggccagttt
     1741 atctagtcag cttgattcta gctgatcgtg gaccggaagg tgagccagtg agttgattgc
     1801 agtccagtta cgctggagtc tgaggctcgt cctgaatgat atgcggcctc gaattcgaag
     1861 acgccatgtc atcctacgta ggagctctcg accagggtac cacctccacc cgtttcattc
     1921 tcttttcgcc tgacggcaag cccgtggcat cccaccagat cgaattcacc cagatctacc
     1981 cccaccccgg atgggtggag cacgaccccg aggagctcgt gagctcgtgt ctggagtgca
     2041 tgtcgtcggt ggccaaggaa atgcgaaccc agggcatcaa ggtggccgac gtgaaggcga
     2101 tcggaatcac caaccagcga gaaaccaccg tgctttggga cattgagacg ggccagcccc
     2161 tgtacaacgc cattgtgtgg tccgacgccc gaaccggcga caccgtcaag aagctcgagg
     2221 cccagcccgg cgctgacgaa atccccaagc tctgtggcct gcccctgtcc acctactttg
     2281 ccggagtcaa ggtccgatgg atcctggata acgtcaagga ggcccgagag tgctacgatc
     2341 gaggcaagct ggccttctcc accatcgact cgtggctgct ctacaacctc acgggcggcc
     2401 tcaacggcgg cgcccacatc accgacacct ccaacgcctc ccggtccatg ttcatgaaca
     2461 ttgagacgct caagtacgac gagaagctca tcaagttctt tggcgtcgag aagctcattc
     2521 tccccaagat tgtctcgtcc gccgaggtct acggccgaat cggaaccggc cccttcgcca
     2581 acatccccct ggccggctgt ctcggtgacc agtccgccgc cctcgtcggc cagaaggcct
     2641 ttgagcccgg ccaggccaag aacacatatg gaaccggctg cttcctgctc tacaacgccg
     2701 gcgagaagcc catcatctcc aacaacggcc tgctgaccac cgtcggctac cacttcaagg
     2761 gccagaagcc cgtctacgct ctggagggct ccatctccgt cgccggctcg tgcatcaagt
     2821 ggctgcgaga caacattggt ctgattgagt catccgagca gattggagag cttgcctccc
     2881 aggtcgacga ctctgccggc gtggtgtttg tcaccgctct gtccggcctg tttgccccct
     2941 actggcgaac cgacgcccga ggcaccattc tgggtctgac tcagttcacc accaaggccc
     3001 acatttgccg agccgccctg gaggctactt gtttccagac ccgggccatt ctcgacgcca
     3061 tggccaagga ctctggtaag cccttcacca agctgcgagt cgacggagga atgaccaact
     3121 cggacattgc tatgcagatc caggccgaca ttcttggcat tgaggtcgag cgacccgcca
     3181 tgcgagagac gaccgctctg ggtgccgcca ttgctgccgg ctttgccgtt ggcgtgtgga
     3241 agtccattga ggatcttaag gacatcaaca ccgagggcat gaccgagttt gcttccaaga
     3301 ccaacgagga ggagcgggcc gccatgatga agcagtggaa ccggggcatt gagcgagctg
     3361 ttggctggct tgagtaagct tgcgtcttcg aattcgcgcg tgatcttacg gcattatacg
     3421 tatgatcggt ccacgatcag ctagatt
//
