LOCUS       sequence-22249-e         639 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Jin J, Li X, Gygi SP, Harper JW
  TITLE     Dual E1 activation systems for ubiquitin differentially regulate E2 
            enzyme charging.
  JOURNAL   Nature. 2007 Jun 28. 447(7148):1135-8.
  PUBMED    17597759
REFERENCE   2  (bases 1 to 639)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2007 Jun 28. 447(7148):1135-8."
COMMENT     Sequence Label: pDEST17-UbE2G2
FEATURES             Location/Qualifiers
     source          1..639
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             39..56
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             60..92
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(96..104,105..119)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     protein_bind    147..171
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tttactttaa gaaggagata tacatatggc ggggttctca tcatcatcat catcatggta
       61 tggctagcat gactggtgga cagcaaatgg gtcgggatct gtacgacgat gacgataagg
      121 atccgagctc gagatctgca gatcaaacaa gtttgtacaa aaaagttggc atggcgggga
      181 ccgcgctcaa gaggctgatg gccgagtaca aacaattaac actgaatcct ccggaaggaa
      241 ttgtagcagg ccccatgaat gaagagaact tttttgaatg ggaggcattg atcatgggcc
      301 cagaagacac ctgctttgag tttggtgttt ttcctgccat cctgagtttc ccacttgatt
      361 acccgttaag tcccccaaag atgagattta cctgtgagat gtttcatccc aacatctacc
      421 ctgatgggag agtctgcatt tccatcctcc acgcgccagg cgatgacccc atgggctacg
      481 agagcagcgc ggagcggtgg agtcctgtgc agagtgtgga gaagatcctg ctgtcggtgg
      541 tgagcatgct ggcagagccc aatgacgaaa gtggagctaa cgtggatgcg tccaaaatgt
      601 ggcgcgatga ccgggagcag ttctataaga ttgccaagc
//
