LOCUS       sequence-222493-        8262 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MAC-tagged gene expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8262)
  AUTHORS   Liu X, Salokas K, Tamene F, Jiu Y, Weldatsadik RG, Ohman T, 
            Varjosalo M
  TITLE     An AP-MS- and BioID-compatible MAC-tag enables comprehensive mapping
            of protein interactions and subcellular localizations.
  JOURNAL   Nat Commun. 2018 Mar 22;9(1):1188. doi: 10.1038/s41467-018-03523-2.
  PUBMED    29568061
REFERENCE   2  (bases 1 to 8262)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-03-22"; volume: "9(1):1188. doi"; pages: " 
            10.1038/s41467-018-03523-2"
COMMENT     Sequence Label: MAC_C_HSP7C
FEATURES             Location/Qualifiers
     source          1..8262
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        112..491
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        492..695
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     646..666
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    697..715
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    718..736
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     746..770
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    913..937
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2876..2900)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2947..2970
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             3007..3030
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             3061..3087
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             3061..3087
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(3088..3435,3436..3438,3439..4047)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(4091..4108)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4097..4321
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4454..4473)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    4605..4652
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             4660..5682
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             4660..5682
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    5812..5933
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5849..5868)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5903..5922
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5982..5998)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5982..5998)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5995..6017)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6006..6022
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6030..6036,6037..6054,6055..6060))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6075..6096
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6213..6230)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6384..6972)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6464..6483)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7143..7934,7935..8003))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7766..7785
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8004..8108)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8183..8202)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta
       61 gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat
      121 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
      181 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
      241 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
      301 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
      361 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
      421 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      481 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      541 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      601 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      661 ggcgtgtacg gtgggaggtc tatataagca gagctctccc tatcagtgat agagatctcc
      721 ctatcagtga tagagatcgt cgacgagctc gtttagtgaa ccgtcagatc gcctggagac
      781 gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc ctccggactc
      841 tagcgtttaa acttaagctt ggtaccgagc tcggatccac tagtccagtg tggtggaatt
      901 ctgcagatat caacaagttt gtacaaaaaa gttggcatgt ccaagggacc tgcagttggt
      961 attgatcttg gcaccaccta ctcttgtgtg ggtgttttcc agcacggaaa agtcgagata
     1021 attgccaatg atcagggaaa ccgaaccact ccaagctatg tcgcctttac ggacactgaa
     1081 cggttgatcg gtgatgccgc aaagaatcaa gttgcaatga accccaccaa cacagttttt
     1141 gatgccaaac gtctgattgg acgcagattt gatgatgctg ttgtccagtc tgatatgaaa
     1201 cattggccct ttatggtggt gaatgatgct ggcaggccca aggtccaagt agaatacaag
     1261 ggagagacca aaagcttcta tccagaggag gtgtcttcta tggttctgac aaagatgaag
     1321 gaaattgcag aagcctacct tgggaagact gttaccaatg ctgtggtcac agtgccagct
     1381 tactttaatg actctcagcg tcaggctacc aaagatgctg gaactattgc tggtctcaat
     1441 gtacttagaa ttattaatga gccaactgct gctgctattg cttacggctt agacaaaaag
     1501 gttggagcag aaagaaacgt gctcatcttt gacctgggag gtggcacttt tgatgtgtca
     1561 atcctcacta ttgaggatgg aatctttgag gtcaagtcta cagctggaga cacccacttg
     1621 ggtggagaag attttgacaa ccgaatggtc aaccatttta ttgctgagtt taagcgcaag
     1681 cataagaagg acatcagtga gaacaagaga gctgtaagac gcctccgtac tgcttgtgaa
     1741 cgtgctaagc gtaccctctc ttccagcacc caggccagta ttgagatcga ttctctctat
     1801 gaaggaatcg acttctatac ctccattacc cgtgcccgat ttgaagaact gaatgctgac
     1861 ctgttccgtg gcaccctgga cccagtagag aaagcccttc gagatgccaa actagacaag
     1921 tcacagattc atgatattgt cctggttggt ggttctactc gtatccccaa gattcagaag
     1981 cttctccaag acttcttcaa tggaaaagaa ctgaataaga gcatcaaccc tgatgaagct
     2041 gttgcttatg gtgcagctgt ccaggcagcc atcttgtctg gagacaagtc tgagaatgtt
     2101 caagatttgc tgctcttgga tgtcactcct ctttcccttg gtattgaaac tgctggtgga
     2161 gtcatgactg tcctcatcaa gcgtaatacc accattccta ccaagcagac acagaccttc
     2221 actacctatt ctgacaacca gcctggtgtg cttattcagg tttatgaagg cgagcgtgcc
     2281 atgacaaagg ataacaacct gcttggcaag tttgaactca caggcatacc tcctgcaccc
     2341 cgaggtgttc ctcagattga agtcactttt gacattgatg ccaatggtat actcaatgtc
     2401 tctgctgtgg acaagagtac gggaaaagag aacaagatta ctatcactaa tgacaagggc
     2461 cgtttgagca aggaagacat tgaacgtatg gtccaggaag ctgagaagta caaagctgaa
     2521 gatgagaagc agagggacaa ggtgtcatcc aagaattcac ttgagtccta tgccttcaac
     2581 atgaaagcaa ctgttgaaga tgagaaactt caaggcaaga ttaacgatga ggacaaacag
     2641 aagattctgg acaagtgtaa tgaaattatc aactggcttg ataagaatca gactgccgag
     2701 aaggaagaat ttgaacatca acagaaagag ctggagaaag tttgcaaccc catcatcacc
     2761 aagctgtacc agagtgcagg aggcatgcca ggaggaatgc ctgggggatt tcctggtggt
     2821 ggagctcctc cctctggtgg tgcttcctca gggcccacca ttgaagaggt tgattaccca
     2881 actttcttgt acaaagtggt tgatatcaag gccgccgaca tcaccagcct gtacaagaaa
     2941 gtggggtgga gccatcccca gttcgagaag ggcggaggat ctggcggcgg aagcggcgga
     3001 ggaagctggt cccaccctca gtttgagaaa ggcaccgagc tgggcagcac catggccagc
     3061 tacccctacg acgtgcccga ctacgccaag gacaacaccg tgcccctgaa gctgatcgcc
     3121 ctgctggcca acggcgagtt ccactctggc gagcagctgg gcgagacact gggcatgagc
     3181 agagccgcca tcaacaagca catccagacc ctgcgggact ggggcgtgga cgtgttcaca
     3241 gtgcccggca agggctacag cctgcccgag cccatccagc tgctgaacgc caagcagatc
     3301 ctgggccagc tggatggcgg atctgtggcc gtgctgcccg tgatcgacag caccaaccag
     3361 tatctgctcg accggatcgg cgagctgaag tccggcgacg cctgtatcgc cgagtaccag
     3421 caggccggca gaggcggcag gggcagaaag tggttcagcc ccttcggcgc caacctgtac
     3481 ctgagcatgt tctggagact ggaacagggc ccagccgccg ctattggcct gagcctggtc
     3541 atcggcatcg tgatggccga ggtgctgcgg aagctgggcg ccgacaaagt gcgcgtgaag
     3601 tggcccaacg acctgtatct gcaggaccgg aagctggccg gcatcctggt ggaactgacc
     3661 ggcaaaacag gcgacgccgc ccagatcgtg atcggcgctg gcatcaacat ggccatgcgg
     3721 cgggtggaag agagcgtggt caaccagggc tggatcaccc tgcaggaagc cggcatcaac
     3781 ctggaccgga acaccctggc cgccatgctg atcagagagc tgagagccgc cctggaactg
     3841 ttcgagcagg aaggcctggc cccctacctg agcagatggg agaagctgga caacttcatc
     3901 aaccggcccg tgaaactgat catcggcgac aaagagatct tcggcatcag ccggggcatc
     3961 gacaagcagg gggccctgct cctggaacag gacggcatca tcaagccctg gatgggcggc
     4021 gagatcagcc tgcggagcgc cgagaagtga tgactcgagt ctagagggcc cgtttaaacc
     4081 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     4141 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     4201 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     4261 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     4321 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc
     4381 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     4441 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     4501 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     4561 ctcgacccca aaaaacttga ttagggtgat ggttcacgta cctagaagtt cctattccga
     4621 agttcctatt ctctagaaag tataggaact tccttggcca aaaagcctga actcaccgcg
     4681 acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct gatgcagctc
     4741 tcggagggcg aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg
     4801 cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg gcactttgca
     4861 tcggccgcgc tcccgattcc ggaagtgctt gacattgggg aattcagcga gagcctgacc
     4921 tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga aaccgaactg
     4981 cccgctgttc tgcagccggt cgcggaggcc atggatgcga tcgctgcggc cgatcttagc
     5041 cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac tacatggcgt
     5101 gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt gatggacgac
     5161 accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc cgaggactgc
     5221 cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca acaatgtcct gacggacaat
     5281 ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc ccaatacgag
     5341 gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta tggagcagca gacgcgctac
     5401 ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc tccgggcgta tatgctccgc
     5461 attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga tgcagcttgg
     5521 gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg gcgtacacaa
     5581 atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtact cgccgatagt
     5641 ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat agcacgtact acgagatttc
     5701 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     5761 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
     5821 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     5881 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     5941 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
     6001 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     6061 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     6121 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     6181 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     6241 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     6301 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     6361 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     6421 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     6481 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     6541 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     6601 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     6661 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     6721 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     6781 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     6841 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     6901 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     6961 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     7021 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     7081 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     7141 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     7201 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     7261 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     7321 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     7381 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     7441 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     7501 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     7561 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     7621 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     7681 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     7741 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     7801 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     7861 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     7921 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     7981 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     8041 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     8101 gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg
     8161 atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc
     8221 tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc ga
//
