LOCUS       sequence-22250-e         564 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 564)
  AUTHORS   Jin J, Li X, Gygi SP, Harper JW
  TITLE     Dual E1 activation systems for ubiquitin differentially regulate E2 
            enzyme charging.
  JOURNAL   Nature. 2007 Jun 28. 447(7148):1135-8.
  PUBMED    17597759
REFERENCE   2  (bases 1 to 564)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2007 Jun 28. 447(7148):1135-8."
COMMENT     Sequence Label: pDEST17-UbE2J2
FEATURES             Location/Qualifiers
     source          1..564
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             4..26
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             46..63
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             67..99
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(103..111,112..126)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     protein_bind    154..178
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atttttgttt aactttaaga aggagatata catatgcggg gttctcatca tcatcatcat
       61 catggtatgg ctagcatgac tggtggacag caaatgggtc gggatctgta cgacgatgac
      121 gataaggatc cgagctcgag atctgcagat caaacaagtt tgtacaaaaa agcaggctcc
      181 gcggccgccc ccttcaccat gagcagcacc agcagtaaga gggctccgac cacggcaacc
      241 cagaggctga agcaggacta ccttcgcatt aagaaagacc cggtgcctta catctgtgcc
      301 gagcccctcc cttcgaatat tctcgagtgg cactatgtcg tccgaggccc agagatgacc
      361 ccttatgaag gtggctatta tcatggaaaa ctaatttttc ccagagaatt tcctttcaaa
      421 cctcccagta tctatatgat cactcccaac gggaggttta agtgcaacac caggctgtgt
      481 ctttctatca cggatttcca cccggacacg tggaacccgg cctggtctgt ctccaccatc
      541 ctgactgggc tcctgagctt catg
//
