LOCUS       sequence-222504-        9604 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Low level expression of mCherry-EGFP-PIM. Can be used for inducible 
            aggregate formation upon AP20187 addition.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9604)
  AUTHORS   Janssen AFJ, Katrukha EA, van Straaten W, Verlhac P, Reggiori F, 
            Kapitein LC
  TITLE     Probing aggrephagy using chemically-induced protein aggregates.
  JOURNAL   Nat Commun. 2018 Oct 12;9(1):4245. doi: 10.1038/s41467-018-06674-4.
  PUBMED    30315152
REFERENCE   2  (bases 1 to 9604)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-10-12"; volume: "9(1):4245. doi"; pages: " 
            10.1038/s41467-018-06674-4"
COMMENT     Sequence Label: pBA-mCherry-EGFP-PIM
FEATURES             Location/Qualifiers
     source          1..9604
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..276
                     /label=chicken beta-actin promoter
     primer_bind     1219..1241
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             1320..1640
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=DmrB
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             1647..1967
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=DmrB
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVEHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDCAYGATGHPGIIPPHTTLVFDVELL
                     KLE"
     CDS             1983..2687
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(2125..2143)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2393..2412
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             join(2697..2699,2700..3410)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(2697..2699,2700..3410)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(2739..2760)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3000..3019)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3347..3368
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4101..4421
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             4428..4748
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /note="synthetic gene with alternative codons"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             4428..4748
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     polyA_signal    5040..5174
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5077..5096)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5131..5150
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5188..5204)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5188..5204)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5201..5223)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5212..5228
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5236..5242,5243..5260,5261..5266))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5281..5302
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5419..5436)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5590..6178)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5670..5689)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6349..7140,7141..7209))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6972..6991
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7210..7314)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7382..7400
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7438..7460)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7560..7579
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7773..7795
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7787..7804
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7788..7804
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa
       61 ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg
      121 cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg
      181 gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg
      241 cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc
      301 cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta
      361 ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta gcgcttggtt
      421 taatgacggc tcgtttcttt tctgtggctg cgtgaaagcc ttaaagggct ccgggagggc
      481 cctttgtgcg ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc
      541 cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg
      601 tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg
      661 gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg
      721 tgtgggcgcg gcggtcgggc tgtaaccccc ccctgcaccc ccctccccga gttgctgagc
      781 acggcccggc ttcgggtgcg gggctccgtg cggggcgtgg cgcggggctc gccgtgccgg
      841 gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg gccggggagg
      901 gctcggggga ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc
      961 gcagccattg ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa
     1021 tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc ctctagcggg cgcggggcga
     1081 agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc
     1141 gccgtcccct tctccatctc cagcctcggg gctgtccgca gggggacggc tgccttcggg
     1201 ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggggtt tatatcttcc
     1261 cttctctgtt cctccgcagc ccccaagcta gcgctaagct tgccaccatg cgctctagag
     1321 gcgtccaagt cgaaaccatt agtcccggcg atggcagaac atttcctaaa aggggacaaa
     1381 catgtgtcgt ccattataca ggcatgttgg aggacggcaa aaaggtggac agtagtagag
     1441 atcgcaataa acctttcaaa ttcatgttgg gaaaacaaga agtcattagg ggatgggagg
     1501 agggcgtggc tcaaatgtcc gtcggccaac gcgctaagct caccatcagc cccgactacg
     1561 catacggcgc taccggacat cccggaatta ttccccctca cgctaccttg gtgtttgacg
     1621 tcgaactgtt gaagctcgag actagaggag tgcaggtgga gactatctcc ccaggagacg
     1681 ggcgcacctt ccccaagcgc ggccagacct gcgtggagca ctacaccggg atgcttgaag
     1741 atggaaagaa agttgattcc tcccgggaca gaaacaagcc ctttaagttt atgctaggca
     1801 agcaggaggt gatccgaggc tgggaagaag gggttgccca gatgagtgtg ggtcagagag
     1861 ccaaactgac tatatctcca gattgtgcct atggtgccac tgggcaccca ggcatcatcc
     1921 caccacatac cactctcgtc ttcgatgtgg agcttctaaa actggaaact agtgaaccgg
     1981 tagtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg
     2041 tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc
     2101 gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct
     2161 tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc
     2221 ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg
     2281 tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg
     2341 gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa
     2401 tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg
     2461 ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg
     2521 aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca
     2581 acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac
     2641 gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagggc gcgcctgtga
     2701 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     2761 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     2821 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     2881 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     2941 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     3001 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     3061 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     3121 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     3181 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     3241 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     3301 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     3361 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag gctagccgct
     3421 cacgcggcgt tcaggtggag actataagcc cgggggatgg tcgcactttt cctaaacgag
     3481 gccagacgtg cgtagttcac tataccggca tgcttgaaga cggcaagaag gtggatagct
     3541 ctagggaccg gaataagccc ttcaagttca tgcttggtaa gcaagaagtg atccggggtt
     3601 gggaagaggg ggttgcccaa atgtcagtcg gacaacgggc gaagttgact atttctcctg
     3661 attacgctta tggtgcgacg ggacatcctg gcatcatacc gcctcacgcc actcttgtct
     3721 ttgatgtgga acttttgaag ctggaaacgc gaggggttca agttgagacc atttcaccgg
     3781 gcgatggcag aacattcccg aaaaggggac aaacgtgtgt agtacactat acgggcatgc
     3841 tcgaagacgg gaaaaaggtt gactcaagta gagatcggaa caagcctttc aagttcatgt
     3901 tggggaaaca agaggtaata cgcgggtggg aggagggagt cgctcagatg tctgtaggcc
     3961 aaagggcgaa gctcaccata tcaccagatt acgcttacgg ggcgacgggc catcccggaa
     4021 taatacctcc gcacgcgaca cttgtttttg atgttgaatt gcttaagttg gagaccagca
     4081 gatctgtcga ccgctctaga ggagtgcagg tggaaaccat ctccccagga gacgggcgca
     4141 ccttccccaa gcgcggccag acctgcgtgg tgcactacac cgggatgctt gaagatggaa
     4201 agaaatttga ctcctcccgg gacagaaaca agccctttaa gtttatgcta ggcaagcagg
     4261 aggtgatccg aggctgggaa gaaggggttg cccagatgag tgtgggtcag agagccaaac
     4321 tgactatatc tccagattat gcctatggtg ccactgggca cccaggcatc atcccaccac
     4381 atgccactct cgtcttcgat gtggagcttc taaaactgga aactagaggc gtgcaggtcg
     4441 agaccatcag ccccggcgac ggccgcacct ttcccaagag aggccagact tgcgtggtcc
     4501 actacaccgg catgctggag gacggcaaga agttcgacag cagccgcgac cgcaacaagc
     4561 ccttcaagtt catgctgggc aaacaggaag tgatccgcgg ctgggaggaa ggcgtggctc
     4621 agatgagcgt ggggcagcgg gccaagctga ccatcagccc cgactatgcc tacggcgcca
     4681 ccggccaccc cggcatcatc cccccccacg ccaccctggt gttcgacgtg gagctgctga
     4741 agctggagac tagttaggaa ttcaggatcc agtcgactag tacttctcga gctctgtaca
     4801 tgtccgcggt cgcgacgtac gcgtatcgat ggcgccagct gcaggcggcc gccatatgca
     4861 tcctaggcct attaatattc cggagtatac gtagccggct aacgttaaca accggtacct
     4921 ctagataagt aatgatcata atcagccata tcacatctgt agaggtttta cttgctttaa
     4981 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
     5041 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     5101 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     5161 atcatgtctg gatcaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc
     5221 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     5281 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     5341 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     5401 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     5461 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     5521 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     5581 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     5641 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     5701 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     5761 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     5821 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     5881 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     5941 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     6001 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     6061 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     6121 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     6181 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     6241 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     6301 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     6361 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     6421 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     6481 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     6541 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     6601 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     6661 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     6721 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     6781 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     6841 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     6901 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     6961 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     7021 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     7081 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     7141 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     7201 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     7261 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     7321 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     7381 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
     7441 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     7501 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
     7561 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
     7621 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga
     7681 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     7741 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
     7801 cagtgccaag ttgggatctt tgcattggcc cacggctctc aggatgggga tgctcccctt
     7861 cagcacccgg ttccccttgg aaactgatgg tcctggctct gtggcatggc agtggcactg
     7921 tgaggagccc cctaccagca gcacacagtg gggttggcac tgccacgctc cggatgccgc
     7981 gctctgatcc aaccccataa tcaagggaac ccgaattgcc ccatcattgc ccccaccacc
     8041 cccatcctgc cgggccctca caccccacgc tgccttctgg tgacattccc cagcccaaac
     8101 ccacggcttc atggctaccg cggggcattt cccattgccg ccccattatc agctctgcac
     8161 acctcccgct gtacccatgc ctcgtggctg cccttctttg acgtataatc ttctaattaa
     8221 tacccggcct tgtcaaagtg gagcacaaac gttaattaat tccccagcag gcaggtaatt
     8281 aacagtgtga ctcccttttt gctgcgagtg gggctgatac agagagatgt ggcactatgg
     8341 agcccacggg gtcctggcac tgggtgccca cggaggtccc catgtgctgc agtgtcaccg
     8401 cctccgagga gacagtattg tccctgcggt gtccctgcag ctcagctctg tccacagggc
     8461 cacctccagt ttggagggga cacaatgcag ccccgatgca acccatcctc gcagcatccc
     8521 agggacaaag accccactgc aagaccgcac acagggctgg gtcccgctcc cctaatatct
     8581 acagtgcttc ttgcatggcc ccttaatcaa tgcagttaat cagcatgcgc tcatgcaccg
     8641 ctctggagct gcaaagcccc tcgcagcgct gctcaccaac accgcgcacc gccccggccc
     8701 agcctgcagc acgcgctgca aacaggaaag aaacaaaata ttgcccaaat gtaggcaaag
     8761 gcattcggct gccttgacct ccgccgggcc gggccctgcc tgactcagct ccttactcag
     8821 cgctcgcttc ctccctccgg ctgccaccgc cgcagcgcac accctgacaa agagtggccc
     8881 ttaacgggct ctgaggtgca cccagcagtg cactcagcag tccaagggcc ggcctggagg
     8941 tttgcaccgc tacgtgctga cattagcatt gaacttggcc ctgggtagtg ctgcaggccg
     9001 ggcggggtgg gtgtagagag tgcagcgcgc gttgcacccg gtgccccttc ccctcccttg
     9061 catcccagca ggctgcaccc cagcaccagg cccgtgcatg catgctcctg gtgttattgc
     9121 agcctggtgc atgcatgcgt cttgatggtg cagcgctgtg catgcatcct ccttggtgtg
     9181 tagcagtcta gtgcatgcat acccctcggt gttattgctg ctctgtgcac gcacgctcat
     9241 tgtatcactt catcccagtg catgcactca cactggagcg attgctgctc ggtgcacgca
     9301 cactcattgt atcactgcag ctcagtggct gcacgcacac cggtgttatt gctgctcggt
     9361 gcgtgcatgc acatcagtgt cgctgcagct cagtgcatgc acgctcattg cccatcgcta
     9421 tccctgcctc tcctgctggc gctccccggg aggtgacttc aaggggaccg caggaccacc
     9481 tcgggggtgg ggggagggct gcacacgcgg accccgctcc ccctccccaa caaagcactg
     9541 tggaatcaaa aaggggggag gggggatgga ggggcgcgtc acacccccgc cccacaccct
     9601 cacc
//
