LOCUS       sequence-222506-       13381 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid encoding the mitochondrial localization of the FRET 
            semi-synthetic biosensor measuring NAD+ concentration in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 13381)
  AUTHORS   Sallin O, Reymond L, Gondrand C, Raith F, Koch B, Johnsson K
  TITLE     Semisynthetic biosensors for mapping cellular concentrations of 
            nicotinamide adenine dinucleotides.
  JOURNAL   eLife. 2018 May 29;7. pii: 32638. doi: 10.7554/eLife.32638.
  PUBMED    29809136
REFERENCE   2  (bases 1 to 13381)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2018-05-29"; volume: "7. pii: 32638. doi"; pages: " 
            10.7554/eLife.32638"
COMMENT     Sequence Label: pEBTet_NAD-Snifit-mito
FEATURES             Location/Qualifiers
     source          1..13381
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(732..750)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        818..922
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(923..991,992..1783)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1141..1160)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1954..2542
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2443..2462
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(2731..2751)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2749..3046
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3123..3722
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3123..3142)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3619..3639
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    3813..3947
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4085..4104)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        4381..4642
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     intron          4759..5331
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     5339..5358
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     5386..5405
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5386..5404
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5415..6035
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(5488..5507)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     polyA_signal    6077..6198
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        6253..6632
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6633..6836
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6787..6807
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    6838..6856
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6859..6877
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     6887..6911
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             7008..7082
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             7095..7166
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="SVLTPLLLRGLTGSARRLPVPRAK"
     CDS             7185..7256
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="SVLTPLLLRGLTGSARRLPVPRAK"
     CDS             7269..7340
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="SVLTPLLLRGLTGSARRLPVPRAK"
     CDS             8163..9047
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEISG"
     primer_bind     complement(9778..9795)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9784..9895
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 tcctgcccct cccgctcctg ctcctgctcc tgttccaccg tgggtccctt tgcagccaat
       61 gcaacttgga cgtttttggg gtctccggac accatctcta tgtcttggcc ctgatcctga
      121 gccgcccggg gctcctggtc ttccgcctcc tcgtcctcgt cctcttcccc gtcctcgtcc
      181 atggttatca ccccctcttc tttgaggtcc actgccgccg gagccttctg gtccagatgt
      241 gtctcccttc tctcctaggc catttccagg tcctgtacct ggcccctcgt cagacatgat
      301 tcacactaaa agagatcaat agacatcttt attagacgac gctcagtgaa tacagggagt
      361 gcagactcct gccccctcca acagcccccc caccctcatc cccttcatgg tcgctgtcag
      421 acagatccag gtctgaaaat tccccatcct ccgaaccatc ctcgtcctca tcaccaatta
      481 ctcgcagccc ggaaaactcc cgctgaacat cctcaagatt tgcgtcctga gcctcaagcc
      541 aggcctcaaa ttcctcgtcc ccctttttgc tggacggtag ggatggggat tctcgggacc
      601 cctcctcttc ctcttcaagg tcaccagaca gagatgctac tggggcaacg gaagaaaagc
      661 tgggtgcggc ctgtgaggat cagcttatcg atgataagct gtcaaacatg agaattcttg
      721 aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
      781 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
      841 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
      901 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
      961 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     1021 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     1081 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     1141 gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat
     1201 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     1261 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     1321 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     1381 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     1441 cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac
     1501 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     1561 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     1621 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     1681 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     1741 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     1801 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     1861 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     1921 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     1981 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     2041 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     2101 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     2161 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     2221 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     2281 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     2341 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     2401 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     2461 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     2521 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     2581 ttgctggcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg
     2641 caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca
     2701 gcctcgcgtc gaactagatg atccggctgt ggaatgtgtg tcagttaggg tgtggaaagt
     2761 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     2821 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     2881 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     2941 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     3001 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggg tgaccgccac
     3061 gaccggtgcc gccaccatcc cctgacccac gcccctgacc cctcacaagg agacgacctt
     3121 ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc ccccgggccg
     3181 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgaccccg
     3241 accgccacat cgaacgcgtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg
     3301 acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg
     3361 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg
     3421 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg
     3481 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg
     3541 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc
     3601 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg
     3661 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct
     3721 gacgcccgcc ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc ggtccgacgc
     3781 ggcccgacgg gtccgagggg ggtcgacctc gaaacttgtt tattgcagct tataatggtt
     3841 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     3901 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatctga tccgaacccc
     3961 ttcctcgacc aattctcatg tttgacagct tatcatcgca gatccgggca acgttgttgc
     4021 cattgctcga ccaattctca tgtttgacag cttatcatcg cagatccgta tggtgcactc
     4081 tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt
     4141 tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg
     4201 acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg
     4261 ccagatatac gcgtatctga ggggactagg gtgtgtttag gcgaaaagcg gggcttcggt
     4321 tgtacgcggt taggagtccc ctcaggatat agtagtttcg cttttgcata gggaggggga
     4381 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
     4441 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
     4501 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
     4561 ccgcattgca gagatattgt atttaagtgc ctagctcgat acaataaacg ccatttgacc
     4621 attcaccaca ttggtgtgca cctccaagct ggccggggat cctctagggc ctctgagcta
     4681 ttccagaagt agtgaagagg cttttttgga ggcctaggct tttgcaaaaa gctccggatc
     4741 gatcctgaga acttcagggt gagtttgggg acccttgatt gttctttctt tttcgctatt
     4801 gtaaaattca tgttatatgg agggggcaaa gttttcaggg tgttgtttag aatgggaaga
     4861 tgtcccttgt atcaccatgg accctcatga taattttgtt tctttcactt tctactctgt
     4921 tgacaaccat tgtctcctct tattttcttt tcattttctg taactttttc gttaaacttt
     4981 agcttgcatt tgtaacgaat ttttaaattc acttttgttt atttgtcaga ttgtaagtac
     5041 tttctctaat cacttttttt tcaaggcaat cagggtatat tatattgtac ttcagcacag
     5101 ttttagagaa caattgttat aattaaatga taaggtagaa tatttctgca tataaattct
     5161 ggctggcgtg gaaatattct tattggtaga aacaactaca tcctggtcat catcctgcct
     5221 ttctctttat ggttacaatg atatacactg tttgagatga ggataaaata ctctgagtcc
     5281 aaaccgggcc cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc
     5341 aacgtgctgg ttattgtgct gtctcatcat tttggcaaag aattgtaata cgactcacta
     5401 tagggcgaat tgatatgtct agattagata aaagtaaagt gattaacagc gcattagagc
     5461 tgcttaatga ggtcggaatc gaaggtttaa caacccgtaa actcgcccag aagctaggtg
     5521 tagagcagcc tacattgtat tggcatgtaa aaaataagcg ggctttgctc gacgccttag
     5581 ccattgagat gttagatagg caccatactc acttttgccc tttagaaggg gaaagctggc
     5641 aagatttttt acgtaataac gctaaaagtt ttagatgtgc tttactaagt catcgcgatg
     5701 gagcaaaagt acatttaggt acacggccta cagaaaaaca gtatgaaact ctcgaaaatc
     5761 aattagcctt tttatgccaa caaggttttt cactagagaa tgcattatat gcactcagcg
     5821 ctgtggggca ttttacttta ggttgcgtat tggaagatca agagcatcaa gtcgctaaag
     5881 aagaaaggga aacacctact actgatagta tgccgccatt attacgacaa gctatcgaat
     5941 tatttgatca ccaaggtgca gagccagcct tcttattcgg ccttgaattg atcatatgcg
     6001 gattagaaaa acaacttaaa tgtgaaagtg ggtccgcgta cagcggatcc cgggaattca
     6061 gatcttatta aagcagaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     6121 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     6181 actcatcaat gtatcttatc atgtctggtc gaatccatgg cgcagaactg gtaggtatgg
     6241 aagatccgcg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
     6301 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
     6361 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
     6421 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
     6481 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
     6541 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     6601 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     6661 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     6721 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     6781 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctctcc
     6841 ctatcagtga tagagatctc cctatcagtg atagagatcg tcgacgagct cgtttagtga
     6901 accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg
     6961 accgatccag cctccggact ctagcgttta aacttaagct taccaccatg tccgtcctga
     7021 cgccgctgct gctgcggggc ttgacaggct cggcccggcg gctcccagtg ccgcgcgcca
     7081 agatccattc gttgtccgtc ctgacgccgc tgctgctgcg gggcttgaca ggctcggccc
     7141 ggcggctccc agtgccgcgc gccaagatcc attcgttggg aggcagcgtg ctcaccccac
     7201 tcctgctgcg gggcttgaca ggctcggccc ggcggctccc agtgccgcgc gctaaaattc
     7261 acagcctctc cgtcctgacg ccgctgctgc tgcggggctt gacaggctcg gcccggcggc
     7321 tcccagtgcc gcgcgccaag atccattcgt tgggaggcag cggaggcagc gagggcgggc
     7381 tggggcgtgc tgtgtgcttg ctgaccgggg cctcccgcgg cttcggccgg acgctggccc
     7441 cgctcctggc ctcgctgctg tcgcccggct ccgtgcttgt ccttagcgac tggaacgacg
     7501 aggcactgcg ccagctggag gccgagctgg gcgccgagcg gtctggcctg cgcgtggtgc
     7561 gggtgcccgc cgacctgggc gccgaggccg gcttgcagca gctgctcggc gccctgcgcg
     7621 agctcccccg gcccaagggg ctgcagcgac tgctgcttat caacaacgcg ggctctcttg
     7681 gggatgtgtc caaaggcttc gtggacctga gtgactccac tcaagtgaac aactactggg
     7741 cactgaactt gacctccatg ctctgcctga cttccagcgt cctgaaggcc ttcccggaca
     7801 gtcctggcct caacagaacc gtggttaaca tctcgtccct ctgtgccctg caacctttca
     7861 aaggctgggc gctgtactgt gcaggaaagg ctgctcgtga tatgctgttc caggtcctgg
     7921 cgctggagga acctaatgtg agggtgctga actatgcccc aggtcctctg gacacagaca
     7981 tgcagcagtt ggcccgggag acctccgtgg acccagacat gcgaaaaggg ctgcaggagc
     8041 tgaaggcaaa ggggaagctg gtggattgca aggtgtcagc ccagaaactg ctgagcttac
     8101 tggaaaagga cgagttcaag tctggagccc acgtggactt ctatgacaaa gaattcggat
     8161 ccgaaatcgg tactggcttt ccattcgacc cccattatgt ggaagtcctg ggcgagcgca
     8221 tgcactacgt cgatgttggt ccgcgcgatg gcacccctgt gctgttcctg cacggtaacc
     8281 cgacctcctc ctacgtgtgg cgcaacatca tcccgcatgt tgcaccgacc catcgctgca
     8341 ttgctccaga cctgatcggt atgggcaaat ccgacaaacc agacctgggt tatttcttcg
     8401 acgaccacgt ccgcttcatg gatgccttca tcgaagccct gggtctggaa gaggtcgtcc
     8461 tggtcattca cgactggggc tccgctctgg gtttccactg ggccaagcgc aatccagagc
     8521 gcgtcaaagg tattgcattt atggagttca tccgccctat cccgacctgg gacgaatggc
     8581 cagaatttgc ccgcgagacc ttccaggcct tccgcaccac cgacgtcggc cgcaagctga
     8641 tcatcgatca gaacgttttt atcgagggta cgctgccgat gggtgtcgtc cgcccgctga
     8701 ctgaagtcga gatggaccat taccgcgagc cgttcctgaa tcctgttgac cgcgagccac
     8761 tgtggcgctt cccaaacgag ctgccaatcg ccggtgagcc agcgaacatc gtcgcgctgg
     8821 tcgaagaata catggactgg ctgcaccagt cccctgtccc gaagctgctg ttctggggca
     8881 ccccaggcgt tctgatccca ccggccgaag ccgctcgcct ggccaaaagc ctgcctaact
     8941 gcaaggctgt ggacatcggc ccgggtctga atctgctgca agaagacaac ccggacctga
     9001 tcggcagcga gatcgcgcgc tggctgtcta ctctggagat ttccggtagc ggccgccctc
     9061 ctcctccgcc accgccacca ccgccgccgc cgccaccacc acctcctccg cctcctccac
     9121 caccaccgcc accgcctcca ccacctggcg gccgctctcg gtctctcgag atggataaag
     9181 actgcgaaat gaaacgcact accctggaca gcccgctggg taaactggaa ctgagcggtt
     9241 gcgagcaggg cctgcacgaa atcattttcc tgggcaaagg tacgagcgct gcggatgcgg
     9301 tcgaagtacc ggctccggct gcggttctgg gtggcccgga gccgctgatg caggctaccg
     9361 catggctgaa cgcatatttt caccagccag aggcgattga agagttccct gtaccggccc
     9421 tgcatcaccc ggtatttcag caggaatcct ttacccgtca ggtactgtgg aaactgctga
     9481 aagtagtgaa attcggcgag gtgatctcct attcccatct ggcggcgctg gctggcaacc
     9541 cagcggcaac tgcggcggtg aaaaccgctc tgtctggtaa tccggtgcca attctgatcc
     9601 cgtgccaccg tgtggttcag ggcgacctgg acgtaggtgg ctacgaaggc ggcctggctg
     9661 tgaaagaatg gctgctggcg cacgaaggtc accgtctggg taaaccaggc ctgggctaaa
     9721 gctagcggcg cgcccggccg ctcgagtcta gagggcccgt ttaaacccgc tgatcagcct
     9781 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     9841 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     9901 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     9961 attgggaaga caatagcagg ctagaggatc gatccccgcc ccggacgaac taaacctgac
    10021 tacgacatct ctgccccttc ttcgcggggc agtgcatgta atcccttcag ttggttggta
    10081 caacttgcca actgggccct gttccacatg tgacacgggg ggggaccaaa cacaaagggg
    10141 ttctctgact gtagttgaca tccttataaa tggatgtgca catttgccaa cactgagtgg
    10201 ctttcatcct ggagcagact ttgcagtctg tggactgcaa cacaacattg cctttatgtg
    10261 taactcttgg ctgaagctct tacaccaatg ctgggggaca tgtacctccc aggggcccag
    10321 gaagactacg ggaggctaca ccaacgtcaa tcagaggggc ctgtgtagct accgataagc
    10381 ggaccctcaa gagggcatta gcaatagtgt ttataaggcc cccttgttaa ccctaaacgg
    10441 gtagcatatg cttcccgggt agtagtatat actatccaga ctaaccctaa ttcaatagca
    10501 tatgttaccc aacgggaagc atatgctatc gaattagggt tagtaaaagg gtcctaagga
    10561 acagcgatat ctcccacccc atgagctgtc acggttttat ttacatgggg tcaggattcc
    10621 acgagggtag tgaaccattt tagtcacaag ggcagtggct gaagatcaag gagcgggcag
    10681 tgaactctcc tgaatcttcg cctgcttctt cattctcctt cgtttagcta atagaataac
    10741 tgctgagttg tgaacagtaa ggtgtatgtg aggtgctcga aaacaaggtt tcaggtgacg
    10801 cccccagaat aaaatttgga cggggggttc agtggtggca ttgtgctatg acaccaatat
    10861 aaccctcaca aaccccttgg gcaataaata ctagtgtagg aatgaaacat tctgaatatc
    10921 tttaacaata gaaatccatg gggtggggac aagccgtaaa gactggatgt ccatctcaca
    10981 cgaatttatg gctatgggca acacataatc ctagtgcaat atgatactgg ggttattaag
    11041 atgtgtccca ggcagggacc aagacaggtg aaccatgttg ttacactcta tttgtaacaa
    11101 ggggaaagag agtggacgcc gacagcagcg gactccactg gttgtctcta acacccccga
    11161 aaattaaacg gggctccacg ccaatggggc ccataaacaa agacaagtgg ccactctttt
    11221 ttttgaaatt gtggagtggg ggcacgcgtc agcccccaca cgccgccctg cggttttgga
    11281 ctgtaaaata agggtgtaat aacttggctg attgtaaccc cgctaaccac tgcggtcaaa
    11341 ccacttgccc acaaaaccac taatggcacc ccggggaata cctgcataag taggtgggcg
    11401 ggccaagata ggggcgcgat tgctgcgatc tggaggacaa attacacaca cttgcgcctg
    11461 agcgccaagc acagggttgt tggtcctcat attcacgagg tcgctgagag cacggtgggc
    11521 taatgttgcc atgggtagca tatactaccc aaatatctgg atagcatatg ctatcctaat
    11581 ctatatctgg gtagcatagg ctatcctaat ctatatctgg gtagtatatg ctatcctaat
    11641 ctgtatccgg gtagcatatg ctatcctaat agagattagg gtagtatatg ctatcctaat
    11701 ttatatctgg gtagcatata ctacccaaat atctggatag catatgctat cctaatctat
    11761 atctgggtag catatgctat cctaatctat atctgggtag catatgctat cctaatctat
    11821 atctgggtag tatatgctat cctaatttat atctgggtag cataggctat cctaatctat
    11881 atctgggtag catatgctat cctaatctat atctgggtag tatatgctat cctaatctgt
    11941 atccgggtag catatgctat cctcatgcat atacagtcag catatgatac ccagtagtag
    12001 agtgggagtg ctatcctttg catatgccgc cacctcccaa gggggcgtga attttcgctg
    12061 cttgtccttt tcctgctggt tgctcccatt cttaggtgaa tttaaggagg ccaggctaaa
    12121 gccgtcgcat gtctgattgc tcaccaggta aatgtcgcta atgttttcca acgcgagaag
    12181 gtgttgagcg cggagctgag tgacgtgaca acatgggtat gcccaattgc cccatgttgg
    12241 gaggacgaaa atggtgacaa gacagatggc cagaaataca ccaacagcac gcatgatgtc
    12301 tactggggat ttattcttta gtgcggggga atacacggct tttaatacga ttgagggcgt
    12361 ctcctaacaa gttacatcac tcctgccctt cctcaccctc atctccatca cctccttcat
    12421 ctccgtcatc tccgtcatca ccctccgcgg cagccccttc caccataggt ggaaaccagg
    12481 gaggcaaatc tactccatcg tcaaagctgc acacagtcac cctgatattg caggtaggag
    12541 cgggctttgt cataacaagg tccttaatcg catccttcaa aacctcagca aatatatgag
    12601 tttgtaaaaa gaccatgaaa taacagacaa tggactccct tagcgggcca ggttgtgggc
    12661 cgggtccagg ggccattcca aaggggagac gactcaatgg tgtaagacga cattgtggaa
    12721 tagcaagggc agttcctcgc cttaggttgt aaagggaggt cttactacct ccatatacga
    12781 acacaccggc gacccaagtt ccttcgtcgg tagtcctttc tacgtgactc ctagccagga
    12841 gagctcttaa accttctgca atgttctcaa atttcgggtt ggaacctcct tgaccacgat
    12901 gctttccaaa ccaccctcct tttttgcgcc tgcctccatc accctgaccc cggggtccag
    12961 tgcttgggcc ttctcctggg tcatctgcgg ggccctgctc tatcgctccc gggggcacgt
    13021 caggctcacc atctgggcca ccttcttggt ggtattcaaa ataatcggct tcccctacag
    13081 ggtggaaaaa tggccttcta cctggagggg gcctgcgcgg tggagacccg gatgatgatg
    13141 actgactact gggactcctg ggcctctttt ctccacgtcc acgacctctc cccctggctc
    13201 tttcacgact tccccccctg gctctttcac gtcctctacc ccggcggcct ccactacctc
    13261 ctcgaccccg gcctccacta cctcctcgac cccggcctcc actgcctcct cgaccccggc
    13321 ctccacctcc tgctcctgcc cctcctgctc ctgcccctcc tcctgctcct gcccctcctg
    13381 c
//
